Identification of Fusarium pathogens of Albizia julibrissin wilt and their cultural characteristics

doi:10.3969/j.issn.1004-1524.2019.04.11

›› 2019, Vol. 31 ›› Issue (4): 588-599.DOI: 10.3969/j.issn.1004-1524.2019.04.11

• Plant Protection • Previous Articles Next Articles

Identification of Fusarium pathogens of Albizia julibrissin wilt and their cultural characteristics

CAI Yue¹, WANG Chuyan¹, HUANG Minghua², CHENG Junwen³, QIAN Zhongyi², GE Chunmei^1,*

1. Department of Biological and Environmental Engineering, Hefei University, Hefei 230601, China;
2. Zhong Hui Ecological Environment Co., Ltd., Hefei 230001, China;
3. Zhejiang Academy of Forestry, Hangzhou 310023, China

Received:2018-09-20 Online:2019-04-25 Published:2019-04-19

Abstract

Abstract: Twenty samples of Albizia julibrissin wilt plants were selected as objects from Changgang, Hefei in Anhui Province, tissue isolation was used and identification was performed on morphological and molecular features, influence of different culture conditions including carbon, nitrogen, pH and temperature on the growth of pathogens were also investigated. Seven strains of Fusarium spp. were obtained and identified as F. equiseti (3 and 3-1), F. oxysporum (4 and 10), F. solani (1-2 and 3-2) and F. proliferatum (2-3), respectively. Pathogenicity tests on the branch showed that F. oxysporum was highly pathogenic strain,while F. solani, F. equiseti and F. proliferatum were weak pathogenic strains. Cultural studies indicated optimum temperature and pH were 28-32 ℃ and 7, respectively, while F. proliferatum could grow faster in pH=9. Sucrose and lactose could be used by F. solani, F. proliferatum and F. equiseti among tested carbons and the optimal carbons of F. oxysporum were glucose, sucrose and lactose. Colony diameters of Fusarium spp. were biggest when ammonium nitrate was used as nitrogen source, and smallest when ammonium oxalate was used as nitrogen source. This study would provide a theoretical basis for the diagnosis and control of Albizia julibrissin wilt.

Key words: Albizia julibrissin wilt, Fusarium, pathogenicity, culture characteristics

CLC Number:

S436.8

CAI Yue, WANG Chuyan, HUANG Minghua, CHENG Junwen, QIAN Zhongyi, GE Chunmei. Identification of Fusarium pathogens of Albizia julibrissin wilt and their cultural characteristics[J]. , 2019, 31(4): 588-599.

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Identification of Fusarium pathogens of Albizia julibrissin wilt and their cultural characteristics

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