Study on screening degrading bacteria and its degradation effect on benzoic acid of autotoxic compounds in strawberry cropping obstacle

doi:10.3969/j.issn.1004-1524.2018.10.13

›› 2018, Vol. 30 ›› Issue (10): 1699-1704.DOI: 10.3969/j.issn.1004-1524.2018.10.13

• Plant Protection • Previous Articles Next Articles

Study on screening degrading bacteria and its degradation effect on benzoic acid of autotoxic compounds in strawberry cropping obstacle

LIU Ziying¹, HUANG Lei², YUAN Bin³, LIU Xiaolin^{3, *}, XU Shengguang⁴, HUANG Tao³

1. College of Chemistry and Bioengineering, Yichun University, Yichun 336000, China;
2. Yichun Ninth Middle School, Yichun 336000, China;
3.College of Life Science and Resources Environment, Yichun University, Yichun 336000, China;
4. Yunnan Urban Agricultural Engineering and Technological Research Center, Kunming University, Kunming 650214, China

Received:2018-01-07 Online:2018-10-25 Published:2018-11-02

Abstract

Abstract: The main reasons of the barrier of strawberry planting were the autotoxic compounds from the soil of strawberry. In order to effectively reduce the harm of the autotoxicity of strawberry, the study was aimed to screen the bacteria with the capacity of degrading benzoic acid. The strains were isolated by using benzoic acid as the sole carbon source and gradually increasing benzoic acid to screen degrading bacteria of strawberry autotoxic compounds. The domestication method was adopted by the ultraviolet spectrophotometry. Antagonistic effects of the effective degradation strain on three strawberry root rot strains were determined. The bacteria were identified by the microbiology characteristics and the sequence analysis of 16S rDNA gene. The degradation rate was 87.5%, which indicated the stain L501 has high efficiency degradation to benzoic acid. Meanwhile, the stain L501 showed strong antagonistic effects against all tested three fungi of strawberry root rot. The stain L501 was identified as Bacillus subtilis, which had double function of degradation benzoic acid and antagonistic.

Key words: strawberry, autotoxicity, cropping obstacle, degrading bacteria, benzoic acid

CLC Number:

S668.4

LIU Ziying, HUANG Lei, YUAN Bin, LIU Xiaolin, XU Shengguang, HUANG Tao. Study on screening degrading bacteria and its degradation effect on benzoic acid of autotoxic compounds in strawberry cropping obstacle[J]. , 2018, 30(10): 1699-1704.

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Study on screening degrading bacteria and its degradation effect on benzoic acid of autotoxic compounds in strawberry cropping obstacle

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