›› 2018, Vol. 30 ›› Issue (11): 1811-1818.DOI: 10.3969/j.issn.1004-1524.2018.11.02

• Animal Science • Previous Articles     Next Articles

Pathogen isolation and identification of cataract disease from Chinese spiny frog (Quasipaa spinosa ) and its histological observation

LIU Ziming1, JIN Jing2, HU Zehui3, DAI Qingmin1, LI Qiaoling1, SHI Qian4, LYU Yaoping1,2,*   

  1. 1. College of Ecology, Lishui University, Lishui 323000, China;
    2. School of Marine Sciences, Ningbo University, Ningbo 315211, China;
    3. Marine Fishery Institute of Zhejiang Province, Zhoushan 316021, China;
    4. Lishui Central Hospital, Lishui 323000, China
  • Received:2018-03-13 Online:2018-11-25 Published:2018-12-21

Abstract: To define the pathogen of cataract disease from Chinese spiny frog (Quasipaa spinosa), the pathogen isolation, artificial infection, physical and chemical characteristics of isolated bacteria and sequence analysis of 16S rDNA were analyzed. The results showed that two pathogens (Mm1 and Mm2) were isolated from the diseased frogs. Healthy Chinese spiny frogs injected the isolated intramuscularly exhibited the similar clinical signs, gross and histopathological lesions to the naturally infected frogs. The isolated strains were identified to be Morganella morganii according to physicochemical properties and the 16S rDNA gene sequence analysis. The strains were sensitive to streptomycin and gentamycin, but resistant to ampicillin and cephalosporin. Histopathological observation found that Morganella morganii infection in Chinese spiny frogs could cause pathological damages, especially in the crystalline lens, liver, intestine, and kidney, accompanied by the emergence of karyolysis, histolysis, and inclusion body in intestine. This study found that Chinese spiny frogs could be infected with Morganella morganii and lead to cataract disease. Biological and pathological characteristics, drug sensitivities were analyzed. These results could provide theoretical basis for the diagnosis and treatment of cataracts disease of Chinese spiny frog.

Key words: Quasipaa spinose, cataract disease, Morganella morganii, histopathology, drug sensitivity test

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