Ultramicroscopical histopathology and pathophysiology of “Shuibiezi” disease of Eriocheir sinensis

doi:10.3969/j.issn.1004-1524.2018.07.06

›› 2018, Vol. 30 ›› Issue (7): 1137-1148.DOI: 10.3969/j.issn.1004-1524.2018.07.06

• Animal Science • Previous Articles Next Articles

Ultramicroscopical histopathology and pathophysiology of “Shuibiezi” disease of Eriocheir sinensis

YANG Zongying^{1, 2}, ZENG Liugen^{1, *}, WANG Yulan¹, YAO Yi¹, HOU Yujie¹, LEI Xiaoqing¹, XU Liangqing¹, XIONG Chunxian¹, YANG Xianle²

1. Nanchang Academy of Agricultural Sciences, Nanchang 330038, China;
2. State Collection Center of Aquatic Pathogen, Shanghai Ocean University, Shanghai 201306, China

Received:2017-12-27 Online:2018-07-20 Published:2018-08-02

Abstract

Abstract: Ultramicroscopical histopathological observations and pathophysiological analysis of Eriocheir sinensis with “Shuibiezi” disease were conducted by the transmission electron microscope technique and biochemical analysis. Ultramicroscopical histopathological results showed that pathological changes in different degrees were observed in gills, muscles and hepatopancreas of diseased crabs. The main pathological characteristics were degenerative and necrotic hepatic cells, decreased number of lipid droplets, and swelled or fractured microvillus. The swelled mitochondria showed abnormal appearance, such as swell, disorganization and reduction or vanish of the crista. The endoplasmic reticula were dilated or fragmented into lamellar structure. As for gills, the chitin layer became thinner, the cell nucleus of epithelial cells of gill was heterochromatinized, the finger shaped protuberance extended from the chitin layer was collapsed and disappeared, microvilli arranged disorderly and fractured or vanished, the sub-cuticular space was expended, the number of mitochondria was decreased and mitochondria was swelled or vacuolar with the decrease of crista, the number of lysosome was increased and lysosome formed autophagosomes with vacuoles and vacuolar mitochondria. Bacteria particles appeared in the severely changed gill epithelium, but no inclusions and other virions. The main pathological changes in muscle cells were relaxed and cracked muscular fibers, melted sarcoplasmic reticulum or formed many small bubbles, the number and volume of mitochondria decreased, and the nuclear was pyknosis and marginalized. Pathophysiological analysis results showed that glucose content and GOT activity in hemolymph of diseased crabs were higher than that of healthy crabs, ALP and GPT activities were significantly higher than those of healthy crabs, whereas triglyceride content was significantly lower than that of healthy crabs. As for hepatopancreas, the liver glycogen content of diseased crabs was significantly lower than that of healthy crabs, and ALP and ACP activities were lower than those of healthy crabs.

Key words: Eriocheir Sinensis, “shuibiezi”, disease;, ultramicroscopical histopathology, pathophysiology

CLC Number:

S945

YANG Zongying, ZENG Liugen, WANG Yulan, YAO Yi, HOU Yujie, LEI Xiaoqing, XU Liangqing, XIONG Chunxian, YANG Xianle. Ultramicroscopical histopathology and pathophysiology of “Shuibiezi” disease of Eriocheir sinensis[J]. , 2018, 30(7): 1137-1148.

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Ultramicroscopical histopathology and pathophysiology of “Shuibiezi” disease of Eriocheir sinensis

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