De novo assembly and functional annotation of transcriptome data of Rhododendron pulchurum cv. Baifeng 4 leaf

doi:10.3969/j.issn.1004-1524.2018.07.07

›› 2018, Vol. 30 ›› Issue (7): 1149-1159.DOI: 10.3969/j.issn.1004-1524.2018.07.07

• Horticultural Science • Previous Articles Next Articles

De novo assembly and functional annotation of transcriptome data of Rhododendron pulchurum cv. Baifeng 4 leaf

WANG Hua¹, WANG Wangwei¹, WANG Dongliang¹, ZHANG Shihu¹, HU Xinfang¹, LU Shiyu¹, GONG Xuemei^{2, *}

1. Department of Ornamental Horticulture, School of Horticulture, Anhui Agricultural University, Hefei 230036, China;
2. Department of Biochemical Engineering, Fuyang Institute of Technology, Fuyang 236031, China

Received:2017-09-18 Online:2018-07-20 Published:2018-08-02

Abstract

Abstract: In this study, the transcriptome analysis of two-years-old cuttage potted seedlings of Rhododendron pulchurum cv. Baifeng 4 were carried out by the second generation of Illumina sequencing technology. The results showed that 213 723 424 Clean reads and 53 568 Unigenes whose average length was 930 nt were obtained after filter, quality control and De novo assembly. Based on it, 28 877 Unigenes were successfully annotated to some databases, such as Nr, Swiss-Prot, KOG (clusters of orthologous group), KEGG (Kyoto encyclopedia of genes and genomes) (E-value≤10^-5). Comparing with the sequence homology of Nr database, it was found that Rhododendron pulchurum had higher homology with Vitis vinifera, but lower homology with other species. There were 30 508 Unigenes from KOG database and divided into 25 categories. GO (gene ontology) analysis showed that 11 928 Unigenes were identified as potentially involved in environmental stresses. From KEGG analysis, 6 478 Unigenes were assigned to 132 known pathways. Among these pathways, 384 Unigenes were related to biosynthesis and transduction of phytohormone. In addition, there were 1 062 Unigenes were annotated as transcription factors, a total of 8 738 SSR markers were excavated, which laid the foundation for further study of Rhododendron.

Key words: Rhododendron pulchurum, transcriptome, sequencing, functional annotation

CLC Number:

S685.21

WANG Hua, WANG Wangwei, WANG Dongliang, ZHANG Shihu, HU Xinfang, LU Shiyu, GONG Xuemei. De novo assembly and functional annotation of transcriptome data of Rhododendron pulchurum cv. Baifeng 4 leaf[J]. , 2018, 30(7): 1149-1159.

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De novo assembly and functional annotation of transcriptome data of Rhododendron pulchurum cv. Baifeng 4 leaf

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