Whole-genome sequencing and antibiotic resistance study of a multi-drug resistant Escherichia coli isolated from chicken

doi:10.3969/j.issn.1004-1524.2019.08.05

›› 2019, Vol. 31 ›› Issue (8): 1249-1256.DOI: 10.3969/j.issn.1004-1524.2019.08.05

• Animal Science • Previous Articles Next Articles

Whole-genome sequencing and antibiotic resistance study of a multi-drug resistant Escherichia coli isolated from chicken

CHANG Jiang^1,2, LUO Yi^2,3, TANG Biao², ZHANG Ling^1,2, DAI Xianjun³, QIU Hanqi^2,3, YANG Hua², XIA Xiaodong^1,*

1. College of Food Science and Engineering, Northwest A & F, Yangling 712100, China;
2. Institute of Quality and Standard for Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
3. College of Modern Science and Technology, China Jiliang University, Hangzhou 310018, China

Received:2019-02-24 Online:2019-08-25 Published:2019-08-30

Abstract

Abstract: An Escherichia coli called ECCNB12-2 isolated from chicken in Ningbo city was studied. The MICs to antibiotic were determined using mircrobroth dilution assay. The results indicated that ECCNB12-2 showed resistance to 10 kinds of antibiotics including ampicillin, gentamicin, spectinomycin, tetracycline, florfenicol, sulfisoxazole, trimethoprim/sulfamethoxazole, ceftiofur, enrofloxacin and ofloxacin. Whole genome sequencing of strain ECCNB12-2 with the third generation of high-throughput sequencing technology was performed. The prediction of acquired antimicrobial resistance genes, virulence factors and mobile genetic elements was carried out based on the whole-genome sequence. The whole genome size of strain ECCNB12-2 was 5 539 489 bp with GC content of 50.37%. At the same time, four plasmids were carried, which had the size of 147 451 bp (pTB-nb1), 139 752 bp (pTB-nb2), 82 252 bp (pTB-nb3) and 253 793 bp (pTB-nb4), respectively. A total of 40 acquired antimicrobial resistance genes were detected on the chromosome, pTB-nb1 and pTB-nb4. And 12 virulence factors were found on the whole-genome sequence. The prediction results of mobile genetic elements showed that the pTB-nb4 had high potential for self-transferability. The study indicated that the ECCNB12-2 isolated from chicken was a multi-drug resistant strain which had a serious risk. It also reflected the severity of antibiotic resistance in commercial chickens. These results provided basic information for risk assessment of antibiotic resistance of foodborne bacterial.

Key words: multi-drug resistance, Escherichia coli, antimicrobial resistance gene, whole-genome sequencing

CLC Number:

S831.7

CHANG Jiang, LUO Yi, TANG Biao, ZHANG Ling, DAI Xianjun, QIU Hanqi, YANG Hua, XIA Xiaodong. Whole-genome sequencing and antibiotic resistance study of a multi-drug resistant Escherichia coli isolated from chicken[J]. , 2019, 31(8): 1249-1256.

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Whole-genome sequencing and antibiotic resistance study of a multi-drug resistant Escherichia coli isolated from chicken

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