Molecular typing, biofilm formation ability and drug resistance of pathogenic Escherichia coli isolated from pigs in Sichuan Province

doi:10.3969/j.issn.1004-1524.2019.10.04

›› 2019, Vol. 31 ›› Issue (10): 1599-1607.DOI: 10.3969/j.issn.1004-1524.2019.10.04

• Animal Science • Previous Articles Next Articles

Molecular typing, biofilm formation ability and drug resistance of pathogenic Escherichia coli isolated from pigs in Sichuan Province

PENG Kenan¹, ZHOU Xueke², YIN Xinhuan¹, LI fei¹, CAI Yao¹, ZENG Yubing¹, JIANG Chaoyuan¹, ZHANG Rubo¹, YANG Zexiao¹, XU Zhiwen^1,3, ZHU Ling^1,3,*

1. College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China;
2. College of Life Sciences, Sichuan University, Chengdu 610044, China;
3. Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China

Received:2019-05-07 Online:2019-10-25 Published:2019-10-30

Abstract

Abstract: The experiment was to study the molecular characteristics and epidemic trends of pathogenic Escherichia coli from pigs in Sichuan Province. From 2016 to 2018, 208 samples of liver, spleen, lung, and intestines of sick pigs were collected from various parts of Sichuan Province. 87 strains of Escherichia coli were screened by bacterial isolation and identification methods, and 22 strains of pathogenic Escherichia coli were determined by SPF mouse challenge test. Molecular grouping, biofilm formation ability detection, pathogenicity and drug resistance of the isolates were studied, drug resistance genes and three HPI virulence genes of pathogenic Escherichia coli were detected by PCR. The results showed that 22 pathogenic Escherichia coli were distributed in group A (59.09%), the positive rate of biofilm formation was 72.73%, and the strong film-forming ability strain accounted for 40.91%. There are different degrees of resistance to 19 antibiotics, among which nine drugs such as enrofloxacin, compound sulfamethoxazole, ampicillin, amoxicillin, erythromycin and tetracycline had higher resistance rates (81.82%-100%). The resistance rates of gentamicin, levofloxacin, norfloxacin and ceftriaxone were between 63.64% and 72.73%. The resistance rate to other drugs was between 4.55% and 54.55%, and all of the 22 isolates were multi-drug resistant. Twelve drug resistance genes detected by 22 pathogenic Escherichia coli include β-lactam TEM(36.36%), SHV(9.09%), OXA(9.09%), sulfonamide sul Ⅰ(63.64%), sul Ⅱ(95.45%), aminoglycosides acc(3)-Ⅱ(68.18%), aph(3')-Ⅱ(50.00%), chloramphenicol cmlA (45.45%), floR(81.82%), tetracycline tetA(81.82%), tetB(86.36%), quinolone qurB(40.91%). Three HPI virulence genes irp1 (40.91%), irp2 (13.64%), and fyuA(13.64%) were detected. It indicated that the pathogenic Escherichia coli from pigs in Sichuan Province was mostly distributed in group A, the positive rate of biofilm formation was high and there was serious multi-drug resistance. The carrying rate of tetracyclines, chloramphenicols, aminoglycosides and sulfonamide-resistant genes were high, and there were multiple drug resistance genes and virulence genes coexisting. The results can provide reference for the prevention and treatment of pig colibacillosis in Sichuan Province.

Key words: Escherichia coli, drug resistance genes, HPI virulence genes, phylogenetic group

CLC Number:

S858.28

PENG Kenan, ZHOU Xueke, YIN Xinhuan, LI fei, CAI Yao, ZENG Yubing, JIANG Chaoyuan, ZHANG Rubo, YANG Zexiao, XU Zhiwen, ZHU Ling. Molecular typing, biofilm formation ability and drug resistance of pathogenic Escherichia coli isolated from pigs in Sichuan Province[J]. , 2019, 31(10): 1599-1607.

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Molecular typing, biofilm formation ability and drug resistance of pathogenic Escherichia coli isolated from pigs in Sichuan Province

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