Isolation, identification and drug resistance detection of a calf Klebsiella pneumoniae strain

doi:10.3969/j.issn.1004-1524.2017.04.03

›› 2017, Vol. 29 ›› Issue (4): 534-541.DOI: 10.3969/j.issn.1004-1524.2017.04.03

• Animal Science • Previous Articles Next Articles

Isolation, identification and drug resistance detection of a calf Klebsiella pneumoniae strain

MENG Zhengqun¹, LENG Yiyi¹, REN Meishen¹, LIU Yadong¹, WANG Yin^{1, 2}, YAO Xueping¹, YANG Zexiao^{1, *}

1. College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, China;
2. Key Laboratory of Animal Disease and Human Health of Sichuan Province,Wenjiang 611130, China

Received:2016-10-26 Online:2017-04-20 Published:2017-04-27

Abstract

Abstract: A gram-negative bacillus was isolated and purified from the lung of a died calf, and was identified by morphological and cultured characteristics, biochemical tests, 16S rDNA gene sequencing, pathogenicity tests, and drug sensitivity tests. Then its drug-resistance genes were detected and analyzed by PCR methods using 7 pairs of primers for drug-resistance genes that produce carbapenemase and extended-spectrum β-lactamase (ESBLs) etc. The results showed that this isolate was identified as cow-derived Klebsiella pneumoniae, which was pathogenic to mice. Its 16S rDNA gene sequence shared 99% homology with Klebsiella pneumoniae gene sequences published in GenBank. The isolate was highly resistant with penbritin, cefuroxime, co-trimoxazole and erythromycin, while sensitive to the gross antibiotics such as carbapenems and the fourth generation cephalosporin. A resistance gene fragment about 862 bp in the length of SHV gene was amplified by PCR, which had a 99.3% homology with published SHV-1 gene sequences in GenBank. The cow-derived Klebsiella pneumoniae strain had strong virulence and drug-resistance, and its drug-resistant phenotype was consistent with the drug-resistant testing results.

Key words: Klebsiella pneumoniae, isolation and identification, drug resistance genes

CLC Number:

S858.23

MENG Zhengqun, LENG Yiyi, REN Meishen, LIU Yadong, WANG Yin, YAO Xueping, YANG Zexiao. Isolation, identification and drug resistance detection of a calf Klebsiella pneumoniae strain[J]. , 2017, 29(4): 534-541.

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Isolation, identification and drug resistance detection of a calf Klebsiella pneumoniae strain

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