Isolation,identification of horse Bacillus cereus and its virulence genes detection

doi:10.3969/j.issn.1004-1524.2019.02.06

›› 2019, Vol. 31 ›› Issue (2): 216-221.DOI: 10.3969/j.issn.1004-1524.2019.02.06

• Animal Science • Previous Articles Next Articles

Isolation,identification of horse Bacillus cereus and its virulence genes detection

CUI Yilong, SHI Yun, YANG Dahan, YIN Youqin, XUE Jiangdong, HUO Xiaowei, MA Dehui^*

1. College of Animal Science and Technology, Inner Mongolia University for the Nationalities, Tongliao 028000, China;
2. Inner Mongolia Engineering Technology Research Center of Prevention and Control the Beef Cattle Disease, Tongliao 028042, China

Received:2018-04-26 Online:2019-02-25 Published:2019-03-06

Abstract

Abstract: In order to identify a pathogenic bacterium isolated from the incidence and death racing of an equestrian club in Tongliao city,Inner Mongolia,the isolated strains were subjected to Gram stain microscopy,animal pathogenicity tests,automatic microbiological analysis system detection,16S rRNA gene amplification,phylogenetic tree analysis and partial virulence gene PCR detection. The results showed that the biochemical characteristics of isolates were consistent with the characteristics of Bacillus cereus(B. cereus); it was sensitive to clindamycin, gentamicin and other drugs, moderately sensitive to cefoxitin, ciprofloxacin and other drugs,and resistant to aztreonam,cefazolin and other drugs; 16S rRNA gene sequence was 100% homologous to the registered B. cereus sequence in GenBank; phylogenetic tree analysis showed that the isolated strains were closest to the relatives of B. cereus;the detection results of virulence gene PCR showed that 8 potential virulence genes entFM,cytK,hblA,hblC,hblD,nheA,nheB and nheC were all positive.The results showed that the isolated strain was a serious pathogenic B. cereus.

Key words: Bacillus cereus, isolation and identification, 16S rRNA, virulence genes

CLC Number:

S858.21

CUI Yilong, SHI Yun, YANG Dahan, YIN Youqin, XUE Jiangdong, HUO Xiaowei, MA Dehui. Isolation,identification of horse Bacillus cereus and its virulence genes detection[J]. , 2019, 31(2): 216-221.

References

[1] 陆湘华,崔昌,王远萍,等. 蜡样芽孢杆菌食物中毒的研究进展[J].传染病信息,2015,28(4):251-254.
LU X H, CUI C, WANG Y P, et al.Research progress of Bacillus cereus foodborne diseases[J].Infectious Disease Information,2015,28(4):251-254.(in Chinese with English abstract)
[2] 赵振宇,戴荣四,刘东友,等.一株猪源致病性蜡样芽孢杆菌的分离与鉴定[J].湖南农业大学学报(自然科学版),2014,6(4):311-315.
ZHAO Z Y,DAI R S,LIU D Y,et al.Isolation and characterization of a pathogenic Bacillus cereus from a piglet with diarrhea[J].Journal of Hunan Agricultural University (Natural Sciences),2014,6(4):311-315.(in Chinese with English abstract)
[3] OZAWA K.Sepsis and meningoencephalitis caused by Bacillus cereus in a patient with myelodysplastic syndrome[J].Internal Medicine,2013,52: 1987-1990.
[4] 庄子慧,何丽,郭云昌,等.我国食源性蜡样芽孢杆菌毒力基因和药物敏感性研究[J].中国食品卫生杂志,2013,25(3):198-201.
ZHUANG Z H,HE L,GUO Y C,et al.Virulent gene profiles and antibiotic susceptibility of foodborne Bacillus cereus in China[J].Chinese Journal of Food Hygiene,2013,25(3):198-201.(in Chinese with English abstract)
[5] 赵超,谷子林,黄军,等. 家兔耐热蜡样芽孢杆菌的分离鉴定和筛选[J].黑龙江畜牧兽医,2009(9):86-88.
ZHAO C,GU Z L,HUANG J,et al. Isolation, identification and screening of rabbit heat-resistant Bacillus cereus[J]. Heilongjiang Animal Science and Veterinary Medicine,2009(9):86-88.(in Chinese)
[6] 蒙显英,黎起秦,冯家勋,等.芽孢杆菌产生的抗菌物质的研究进展[J].中国植保导刊,2004,24(12) : 13-15.
MENG X Y,LI Q Q,FENG J X,et al.Advances in research on antibacterial substances produced by Bacillus cereus[J].China Plant Protection,2004,24(12) : 13-15.(in Chinese)
[7] 王君. 全国食品中蜡样芽孢杆菌的污染分布规律及遗传多样性研究[D].广州:广东工业大学,2013.
WANG J.Study on contamination distribution and genetic diversity of Bacillus cereus isolated from food in China[D]. Guangzhou: Guangdong University of Technology,2013.(in Chinese with English abstract)
[8] AGATAN, OHTA M, ARAKSWA Y, et al.The becT gene of Bacillus cereus encodes an enterotoxin protein[J].Microbiology, 1995,141(Pt4):983-988.
[9] HARDY S P, LUND T, GRANUM P E.CytK toxin of Bacillus cereus forms pores in planar lipid bilayers and is cytotoxic to intestinal epithelia[J].FEMS Microbiology Letters,2001,197(1):47-51.
[10] LUND T, GRANUM P E.The 105-kDa protein component of Bacillus cereus nonhaemolytic enterotoxin (Nhe) is a metalloprotease with gelatinolytic and collagenolytic activity[J].FEMS Microbiology Letters,1999,178(2):355-361.
[11] 何文艳,裘娟萍,赵春田.蜡状芽孢杆菌毒株的致病机理及检测方法[J].科技通报,2012,28(1):86-90.
HE W Y,QIU J P,ZHAO C T.Pathogensis mechanism and detection methods of Bacillus cereus[J].Bulletin of Science and Technology,2012,28(1):86-90.(in Chinese with English abstract)
[12] 秦丽云,吕国平,蔡箴,等.石家庄市131株食源性蜡样芽胞杆菌毒力基因的分布[J].中国食品卫生杂志,2015,27(4):358-362.
QIN L Y,LYU G P,CAI J,et al.Distribution of virulence genes and virulence evaluation of food-borne Bacillus cereus in Shijiazhuang City[J].Chinese Journal of Food Hygiene,2015,27(4):358-362.(in Chinese with English abstract)

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Isolation,identification of horse Bacillus cereus and its virulence genes detection

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