Preliminary study on hemolytic ascites disease of Pelteobagrus fulvidraco

doi:10.3969/j.issn.1004-1524.2019.08.04

›› 2019, Vol. 31 ›› Issue (8): 1239-1248.DOI: 10.3969/j.issn.1004-1524.2019.08.04

• Animal Science • Previous Articles Next Articles

Preliminary study on hemolytic ascites disease of Pelteobagrus fulvidraco

YANG Yibin^1,2,3, AI Xiaohui^2,3, SONG Yi³, DONG Jing^2,3, XU Ning^2,3, JIANG Lan^1,*

1. Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China;
2. Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China;
3. Key Laboratory of Control of Quality and Safety for Aquatic Products, Minisrty of Agriculture and Rural Affairs, Beijing 100141, China

Received:2019-02-26 Online:2019-08-25 Published:2019-08-30

Abstract

Abstract: Bacterial strain HS01 was isolated from diseased Pelteobagrus fulvidraco suffered from serious hemolytic ascites, and it was identified as Aeromonas hydrophila based on biochemical reaction characters and 16S rRNA and gyrB gene sequence analyses. Artificial affected fish by intraperitoneal HS01-injection showed the same clinic signs as the diseased fish in ponds. Therefore, the isolated A.hydrophila was proved as the main pathogen, and its LD₅₀ to tilapia was determined as 2.51×10⁵ CFU·g^-1 using the probabilistic graphical method. The antibiotic sensitivity test showed that the isolated was very sensitive to cefotaximee, cefotaxime, netilmicin, florfenicol, etc., less sensitive to cefadroxil, streptomycin1, neomycin, kanamycin, etc., and not sensitive to amoxicillin, penicillin, tobramycin, erythrocin. This study provided a theoretical basis for the prevention and control of the hemorrhagic disease caused by A.hydrophila in Pelteobagrus fulvidraco.

Key words: Pelteobagrus fulvidraco, Aeromonas hydrophila, isolation and identification, pathology, 16S rDNA high-throughput sequencing technology

CLC Number:

S941.42

YANG Yibin, AI Xiaohui, SONG Yi, DONG Jing, XU Ning, JIANG Lan. Preliminary study on hemolytic ascites disease of Pelteobagrus fulvidraco[J]. , 2019, 31(8): 1239-1248.

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Preliminary study on hemolytic ascites disease of Pelteobagrus fulvidraco

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