Isolation, identification and antibiotic sensitivity of Streptococcus iniae from Pelteobagrus fulvidraco

doi:10.3969/j.issn.1004-1524.2017.06.07

›› 2017, Vol. 29 ›› Issue (6): 903-909.DOI: 10.3969/j.issn.1004-1524.2017.06.07

• Animal Science • Previous Articles Next Articles

Isolation, identification and antibiotic sensitivity of Streptococcus iniae from Pelteobagrus fulvidraco

YANG Yibin^1,2, XU Ning^1,2, YANG Qiuhong^1,2, LIU Yongtao^1,2, DONG Jing^1,2, AI Xiaohui^1,2,*

1. Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China;
2. Hubei Freshwater Aquaculture Collaborative Innovation Center, Wuhan 430223, China

Received:2016-12-13 Online:2017-06-20 Published:2017-09-07

Abstract

Abstract: The study was aimed to investigate the cause about the outbreak of numerous deaths of Pelteobagrus fulvidraco at a fishery farm in Zhangzhou, Fujian Province, and to provide reference for the prevention and control of the disease for Pelteobagrus fulvidraco. The pathogenic bacteria were isolated and purified from liver, kidney and spleen of sick Pelteobagrus fulvidraco. And the identification of the strain si222 was conducted, using the biochemical identification and 16S rRNA gene sequence determination. The artificial infection test was performed, and the antimicrobial susceptibility test was conducted by disc diffusion method. The results showed that strain si222 was the pathogens of Pelteobagrus fulvidraco, the LD₅₀ of the isolate was counted to 2.63×10⁴ cfu·g^-1. According to morphological and biochemical characteristics as well as the result of 16S rRNA gene sequence analysis, the isolated strain si222 was Streptococcus iniae. Strain si222 was susceptible to oxacillin, cefadroxil, clindamycin and rifampicin and other 11 kinds of antibiotics. Meanwhile, it showed resistance to penicillin, amoxicillin, sulfisoxazole, florfenicol and other 6 kinds of antibiotics. The isolated strain si222 was the pathogenic bacteria to Pelteobagrus fulvidraco, and the disease can be prevented by administering drugs such as gentamicin and doxycycline in fisheries farming.

Key words: Streptococcus iniae, Pelteobagrus fulvidraco, artificial infection, antibiotic sensitivity

CLC Number:

S941.42

YANG Yibin, XU Ning, YANG Qiuhong, LIU Yongtao, DONG Jing, AI Xiaohui. Isolation, identification and antibiotic sensitivity of Streptococcus iniae from Pelteobagrus fulvidraco[J]. , 2017, 29(6): 903-909.

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Isolation, identification and antibiotic sensitivity of Streptococcus iniae from Pelteobagrus fulvidraco

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