›› 2018, Vol. 30 ›› Issue (11): 1825-1831.DOI: 10.3969/j.issn.1004-1524.2018.11.04

• Animal Science • Previous Articles     Next Articles

Molecular identification of genus Culicoides (Diptera: Ceratopogonidae) based on mtDNA CO Ⅰ gene

CEN Changhuo, XU Zongyi, HAN Xiaojing, CHANG Qiongqiong, DUAN Chen, HOU Xiaohui*   

  1. Zunyi Medical University, Zunyi 563000, China
  • Received:2018-03-05 Online:2018-11-25 Published:2018-12-21

Abstract: In order to make up for the deficiency of complex operation and big difficulty effectively in the traditional morphological species identification of sibling species of blood-sucking midges and differentiate these rapidly and accurately, molecular identification of seven closely related species of the genus Culicoides, C. pelius, C. innoxius, C. albifascia, C. obsoletus, C. orientalis, C. scoticus and C. actoni were studied based on mitochondrial cytochrome c oxidase subunit I gene (CO Ⅰ). Approximately 493 bp of CO Ⅰ gene were sequenced for those 7 species. Estimate genetic distances of CO Ⅰ gene, and statistical analysis showed that intraspecific genetic distances were different from that of interspecies significantly (P<0.05). The phylogenetic relationship within Culicoides indicates that the topologies of all the phylogenetic trees were quite similar. Distinguished between different species of Culicoides clearly, no cross between different species. The same species gathered closely with each other. These results were consistent with those of morphological taxonomy.

Key words: Ceratopogonidae, biting midges, subgenus Avaritia, sibling species, CO I gene

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