Evaluation of immune efficacy of inactivated Streptococcus suis serotype 2 vaccine in mice

doi:10.3969/j.issn.1004-1524.2020.04.03

›› 2020, Vol. 32 ›› Issue (4): 577-585.DOI: 10.3969/j.issn.1004-1524.2020.04.03

• Animal Science • Previous Articles Next Articles

Evaluation of immune efficacy of inactivated Streptococcus suis serotype 2 vaccine in mice

DUAN Qianqian, MAO Tianjiao, HAN Yeqin, HAN Xuejiao, WEI Jianzhong, SUN Pei, LI Yu^*

College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China

Received:2019-09-14 Online:2020-04-25 Published:2020-04-26

Abstract

Abstract: In preliminary research, 2 vaccine strains of Streptococcus suis serotype 2 HF2, HF3 were screened out through pathogenicity test, antigenicity test and stability test, and been prepared into inactivated vaccine with ISA 201 VG mineral oil adjuvant. These two inactivated vaccines were immunized in mice with commercial vaccine to evaluate their immune efficacy. The titer of IgG antibody and content of cytokines (IL-4, IL-10, IFN-γ, TNF-β, MCP-1) in serum were detected by ELISA and the subpopulation of T cells of CD4⁺/CD3⁺, CD8⁺/CD3⁺ were measured by flow cytometry. The protective rate of each immunization group after challenge test, the colonization of SS2 in mice by tissue load test and the pathological sections of lung, liver, spleen and kidney of mice were collected and observed. The results showed that the serum IgG antibody titers of the SS2 inactivated vaccine (HF2 strain, HF3 strain) and the SS2 commercial inactivated vaccine (HA9801 strain) after the second immunization of mice were 1:25 600, 1:12 800, 1:25 600, respectively. The content of IL-4 and IL-10 induced by HF2 inactivated vaccine were significantly higher than HF3 inactivated vaccine and commercial vaccine. The content of IFN-γ、TNF-β were significantly higher than HF3 inactivated vaccine, and the content of MCP-1 were significantly lower than HF3 inactivated vaccine. The commercial vaccine group induced mice to produce significantly higher ratio of CD4⁺/CD3⁺ T cells than other groups. The protection rate of HF2, HF3 inactivated vaccine and HA9801 commercial inactivated vaccine on mice were all 100%.The bacteria colonization of lung and spleen of HF2 inactivated vaccine were significantly lower than HF3 inactivated vaccine, and the pathological changes of lung, spleen and kidney were lighter than other groups. In conclusion, HF2 inactivated vaccine can not only produce a higher level of humoral immunity and cellular immunity, but also completely resist the attack of the virulent strain.

Key words: Streptococcus suis serotype 2, inactivated vaccine, immune efficacy, mice

CLC Number:

S855.3

DUAN Qianqian, MAO Tianjiao, HAN Yeqin, HAN Xuejiao, WEI Jianzhong, SUN Pei, LI Yu. Evaluation of immune efficacy of inactivated Streptococcus suis serotype 2 vaccine in mice[J]. , 2020, 32(4): 577-585.

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Evaluation of immune efficacy of inactivated Streptococcus suis serotype 2 vaccine in mice

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