Correlation analysis of growth traits and two microsatellite polymorphisms in flanking region of growth hormone gene in topmouth culter (Culter alburnus Basilewsky)

doi:10.3969/j.issn.1004-1524.2019.01.08

›› 2019, Vol. 31 ›› Issue (1): 62-68.DOI: 10.3969/j.issn.1004-1524.2019.01.08

• OAnimal Science • Previous Articles Next Articles

Correlation analysis of growth traits and two microsatellite polymorphisms in flanking region of growth hormone gene in topmouth culter (Culter alburnus Basilewsky)

LIU Shili^1,2,3,4, JIANG Wenping¹, CHENG Shun¹, CHI Meili¹, ZHENG Jianbo¹, JIA Yongyi^1,*, ZHAO Jinliang^{2,3, 4}, GU Zhimin¹

1. Key Laboratory of Healthy Freshwater Aquaculture/Key Laboratory of Freshwater Aquatic Animal Genetic and Breeding of Zhejiang Province, Ministry of Agriculture and Rural Affairs, Zhejiang Institute of Freshwater Fisheries, Huzhou 313001, China;
2. Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China;
3. Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, China;
4. National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China

Received:2018-06-27 Online:2019-01-25 Published:2019-04-09

Abstract

Abstract: The body length and weight of 120 topmouth culter (Culter alburnus Basilewsky) cultured in the same pond were measured. The polymorphism information and correlation with growth traits of two microsatellite loci, Cal-GH01 and Cal-GH02, located in the flanking regions of the growth hormone (GH) gene were analyzed. The results showed that three Cal-GH01 alleles (404, 407 and 410 bp) and six genotypes could be detected. The 407 bp allele was the dominant allele, and 407/407 bp was the dominant genotype. The polymorphic information content of the microsatellite locus was 0.420, and the heterozygosity was 0.207 2. A total of six alleles (366, 375, 378, 381, 384 and 387 bp) and 11 genotypes were detected for Cal-GH02. The 381 bp allele was the dominant allele, and 381/381 bp was the dominant genotype. The polymorphic information content of the microsatellite locus was 0.450, and the heterozygosity was 0.466 1, which made it a moderately polymorphic locus. The associations between the polymorphisms of the two loci and growth traits were analyzed using general linear models. The analysis results for microsatellite locus Cal-GH01 showed that 404/407-type individuals accounted for 13.3% of the sample size and had the longest body length. The 407/410-type individuals accounted for 5% of the sample weight and were the heaviest. There were some differences in body length and body weight among the Cal-GH01 genotypes; however, the differences were not significant (P>0.05). For microsatellite locus Cal-GH02, 366/381-type individuals accounted for 3.3% of the number of samples, and their body length and body weight were the highest. Their weight was significantly higher than that of the other genotypes (P<0.05). 384/387-type individuals accounted for 2.5% of the sample size, and their body length and weight were the lowest. Their body length was significantly lower than that of other genotypes (P<0.05). In conclusion, we studied the genetic characteristics of two microsatellite loci in 120 fish sampled from the same pond. The correlation analysis for body length and body weight provided a scientific basis for marker-assisted selection for growth traits. Overall, there were associations between polymorphisms in the GH gene and the growth traits of C. alburnus. Therefore, the two microsatellite loci in GH gene could be used as markers for practical breeding programs for growth traits in C. alburnus.

Key words: Culter alburnus, growth hormone, microsatellite, growth trait, genetic association

CLC Number:

LIU Shili, JIANG Wenping, CHENG Shun, CHI Meili, ZHENG Jianbo, JIA Yongyi, ZHAO Jinliang, GU Zhimin. Correlation analysis of growth traits and two microsatellite polymorphisms in flanking region of growth hormone gene in topmouth culter (Culter alburnus Basilewsky)[J]. , 2019, 31(1): 62-68.

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Correlation analysis of growth traits and two microsatellite polymorphisms in flanking region of growth hormone gene in topmouth culter (Culter alburnus Basilewsky)

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