Molecular detection of Bartonella infection in ixodid ticks collected from yaks in Shiqu County of Sichuan Province

doi:10.3969/j.issn.1004-1524.2019.07.05

›› 2019, Vol. 31 ›› Issue (7): 1066-1072.DOI: 10.3969/j.issn.1004-1524.2019.07.05

• Animal Science • Previous Articles Next Articles

Molecular detection of Bartonella infection in ixodid ticks collected from yaks in Shiqu County of Sichuan Province

TANG Tiancai¹, LIU Chengcheng¹, YUAN Dongbo², GUO Li², HOU Wei², MO Xi², YANG Aiguo², HAO Lili^1,*, LI Rui³

1. College of Life Science & Technology, Southwest Minzu University, Chengdu 610041, China;
2. Center for Animal Disease Control and Prevention in Sichuan Province, Chengdu 610041, China;
3. Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2019-02-07 Online:2019-07-25 Published:2019-08-07

Abstract

Abstract: In order to investigate tick species and its Bartonella infection from yaks in Shiqu County of Sichuan Province, the ticks collected from yaks were classified by morphological identification. The total DNA of ticks was extracted and partial sequences of COⅠ and gltA gene of ticks and Bartonella were amplified by PCR, respectively. The positive products were sequenced and compared through the NCBI database. Phylogenetic tree was constructed based on COⅠ and gltA for determination species of ticks and Bartonella, respectively. A total of 818 ticks were collected from 4 villages in Shiqu County and only Dermacentor everestianus (78.97%, 646/818) and Haemphysalis qinghaiensis (21.03%, 172/818) were found. The total infection rate of Bartonella in ticks was 30.07% and the infection rates of Arizha, Maga, Derongma and Changxuganma townships were 4.76%, 76.79%, 12.50% and 17.95%, respectively. Compared with other villages, the infection rate of Bartonella in Maga was higher (P<0.01). In Maga, no significant difference was observed (P>0.05), though the infection rate of Bartonella in Haemphysalis qinghaiensis(79.07%)was higher than that in Dermacentor everestianus (69.23%). At last, three sequences of gltA were obtained (uncultured Bartonella sp. shiqu 1, uncultured Bartonella sp. shiqu 2 and uncultured Bartonella sp. Shiqu 3). Phylogenetic analysis showed that uncultured Bartonella sp. Shiqu 1 and uncultured Bartonella sp. Shiqu 2 had the closest relationship with the undetermined Bartonella spp. RF124HAIN (FJ464240) and uncultured Bartonella sp. Shiqu 3 had the closest relationship with the human pathogenic Bartonella melophagi. At present, Dermacentor everestianus and Haemphysalis qinghaiensis were found in Shiqu County with high infection of Bartonella, and there existed high risk of human infection.

Key words: Shiqu County, yak, ticks, Bartonella

CLC Number:

S855.9⁺9

TANG Tiancai, LIU Chengcheng, YUAN Dongbo, GUO Li, HOU Wei, MO Xi, YANG Aiguo, HAO Lili, LI Rui. Molecular detection of Bartonella infection in ixodid ticks collected from yaks in Shiqu County of Sichuan Province[J]. , 2019, 31(7): 1066-1072.

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Molecular detection of Bartonella infection in ixodid ticks collected from yaks in Shiqu County of Sichuan Province

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