Inhibition effect of Ageratina adenophorais methanol extracts against Microsporidium gypsum and its mechanism

doi:10.3969/j.issn.1004-1524.2019.09.21

›› 2019, Vol. 31 ›› Issue (9): 1555-1562.DOI: 10.3969/j.issn.1004-1524.2019.09.21

• Biosystems Engineering • Previous Articles Next Articles

Inhibition effect of Ageratina adenophorais methanol extracts against Microsporidium gypsum and its mechanism

HU Liwen, ZHANG Yong, SHI Zhen, FU Jie, SUN Wei, DENG Junliang, REN Zhihua, ZUO Zhicai, CAO Suizhong, HU Yanchun^*

College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China

Received:2019-05-25 Online:2019-09-25 Published:2019-10-11

Abstract

Abstract: In the present study, 9-oxo-10,11-dehydroagerophorone (euptox A), cadinan-3-ene-2,7-dione (CED) and olefin compounds were separated and identified from Ageratina adenophorais methanol extract by silica gel column chromatography, thin-layer-chromatography and gas chromatography-mass spectrometer. The inhibition effects of 125, 62.5, 31.25, 15.6, 7.8, 3.9, 1.95 and 0.98 μg·mL^-1 euptox A and CED against Microsporidium gypsum were determined at 48, 72, 96, 120, 144 h, respectively. The Microsporidium gypsum samples treated with euptox A were selected as test materials, dye penetration test with propidium iodide (PI) was carried out, and the content of total protein and total phosphorus in extra-cellular fluid, and ergo-sterol content in mycelium were determined. It was shown that the separated euptox A and CED from Ageratina adenophorais methanol extract could inhibit the growth of Microsporidium gypsum, and euptox A possessed stronger inhibition effect. After the treatment with euptox A, the hyphae of Microsporidium gypsum showed strong red fluorescence under fluorescence microscope. The contents of total protein and total phosphorus in extracellular fluid of Microsporidium gypsum were significantly (P<0.05) increased, yet the ergo-sterol content in mycelium was significantly (P<0.05) decreased. It was inferred that euptox A could inhibit the synthesis of ergo-sterol, cause membrane damage, and lead to cellular disorder and cell death.

Key words: Ageratina adenophorais, antifungal activity, antifungal mechanism

CLC Number:

S859.1

HU Liwen, ZHANG Yong, SHI Zhen, FU Jie, SUN Wei, DENG Junliang, REN Zhihua, ZUO Zhicai, CAO Suizhong, HU Yanchun. Inhibition effect of Ageratina adenophorais methanol extracts against Microsporidium gypsum and its mechanism[J]. , 2019, 31(9): 1555-1562.

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Inhibition effect of Ageratina adenophorais methanol extracts against Microsporidium gypsum and its mechanism

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