Screening of immunological adjuvant for inactivated vaccine against Erysipelothrix rhusiopathiae

doi:10.3969/j.issn.1004-1524.2019.11.05

›› 2019, Vol. 31 ›› Issue (11): 1803-1811.DOI: 10.3969/j.issn.1004-1524.2019.11.05

• Animal Science • Previous Articles Next Articles

Screening of immunological adjuvant for inactivated vaccine against Erysipelothrix rhusiopathiae

CHEN Zhang¹, LIU Xiaolu¹, YAO Yanbin¹, WU Qiongjuan¹, SUN Pei¹, WEI Jianzhong¹, LI Dongfeng², LI Yu^1,*

1.College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China;
2.Veterinary Drug and Feed Surveillance Institute of Anhui Province, Hefei 230001, China

Received:2018-10-30 Online:2019-11-25 Published:2019-12-04

Abstract

Abstract: To screen out the best immunoadjuvant for Erysipelothrix rhusiopathiae inactivated vaccine, five adjuvants (polymer adjuvant, mineral oil adjuvant ISA 201 VG, propolis adjuvant, Freund's adjuvant, aluminum gel adjuvant) were used to prepare 15 inactivated vaccines with three strains of Erysipelothrix rhusiopathiae(AEr21, AEr31 and AER32), respectively. Mice were immunized by the vaccines which had passed physical property, sterility and safety test, serum antibody IgG and cytokines (IL-4, IL-10, TNF-β, IFN-γ, MCP-1) levels were measured by ELISA. Calculating the protective rate of each immunization group after challenge by intraperitoneal injection and gavage. The results showed that all the inactivated vaccines prepared by five adjuvants and three strains could stimulate mice to produce good humoral and cellular immunity. The levels of IgG and cytokines induced by mineral oil adjuvant ISA 201 VG vaccine in mice were the highest, which was significantly different from that of aluminum gel adjuvant and Freund's adjuvant vaccine (P<0.05), but not significantly different from that of polymer adjuvant and propolis adjuvant vaccine(P>0.05). The mineral oil adjuvant ISA 201 VG vaccine had the highest immune protection rate after challenge, reaching 100%、100%、80% for intraperitoneal injection and 80%、80%、60% for gavage, respectively. The results showed that mineral oil adjuvant ISA 201 VG could be used as a candidate immunoadjuvant for the preparation of inactivated vaccine against Erysipelothrix rhusiopathiae.

Key words: Erysipelothrix rhusiopathiae, immunoadjuvant, inactivated vaccine, immune efficacy, mice

CLC Number:

S858.28

CHEN Zhang, LIU Xiaolu, YAO Yanbin, WU Qiongjuan, SUN Pei, WEI Jianzhong, LI Dongfeng, LI Yu. Screening of immunological adjuvant for inactivated vaccine against Erysipelothrix rhusiopathiae[J]. , 2019, 31(11): 1803-1811.

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Screening of immunological adjuvant for inactivated vaccine against Erysipelothrix rhusiopathiae

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