Construction of DNA fingerprinting of Zhejiang eggplant varieties

doi:10.3969/j.issn.1004-1524.2019.11.12

›› 2019, Vol. 31 ›› Issue (11): 1863-1870.DOI: 10.3969/j.issn.1004-1524.2019.11.12

• Horticultural Science • Previous Articles Next Articles

Construction of DNA fingerprinting of Zhejiang eggplant varieties

WEI Qingzhen, WANG Wuhong, HU Tianhua, HU Haijiao, WANG Jinglei, BAO Chonglai^*

Institute of Vegetable, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2019-07-09 Online:2019-11-25 Published:2019-12-04

Abstract

Abstract: In order to effectively and quickly identify and distinguish eggplant varieties of Zhejiang eggplant, and to provide the basis for the identification of the specificity and authenticity of Zhejiang eggplant varieties, SNP markers developed from eggplant genome data were screened, and SNP fingerprints were constructed for 6 eggplant varieties of Zhejiang eggplant type. Results showed that 48 SNPS were converted into KASP markers, and then used to KASP genotyping for 46 eggplant materials. According to the genotyping results and polymorphism information, 34 SNP markers could be used as core markers, which were used to construct a fingerprint map of the 6 Zhejiang eggplant varieties. The fingerprint map constructed herein could provide theoretical basis for eggplant cultivar identification and the protection of intellectual property.

Key words: eggplant, core varieties, SNP marker, fingerprinting

CLC Number:

S641.1

WEI Qingzhen, WANG Wuhong, HU Tianhua, HU Haijiao, WANG Jinglei, BAO Chonglai. Construction of DNA fingerprinting of Zhejiang eggplant varieties[J]. , 2019, 31(11): 1863-1870.

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Construction of DNA fingerprinting of Zhejiang eggplant varieties

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