Analysis on cp gene sequences of potato virus Y from some places in Qinghai Province

doi:10.3969/j.issn.1004-1524.2019.11.15

›› 2019, Vol. 31 ›› Issue (11): 1888-1895.DOI: 10.3969/j.issn.1004-1524.2019.11.15

• Plant Protection • Previous Articles Next Articles

Analysis on cp gene sequences of potato virus Y from some places in Qinghai Province

CHENG Liang^1,2

1. Key Laboratory of Agricultural Integrated Pest Management in Qinghai Province, Institute of Plant Protection, Qinghai Provincial Academy of Agriculture and Forestry Sciences, Xining 810016, China;
2. State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining 810016, China

Received:2019-04-08 Online:2019-11-25 Published:2019-12-04

Abstract

Abstract: Potato virus Y(PVY)is one of the main viruses, which can cause serious losses in potato production. PVY is prone to gene mutation and plant differentiation under natural conditions. To understand the sequence variation of cp gene in PVY, cp gene of 16 isolates from Qinghai was amplified by RT-PCR. SDT, DNAMAN, RDP, and MEGA software were used for analysis. In multiple sequence alignments of cp gene, 16 isolates contained 801 base pairs with the identity of 98.0%-100% and encoded 267 amino acides with the identity of 94.8%-100%, respectively. Recombinant analysis indicated that no gene recombination was found in 16 isolates. Phylogenetic analysis showed that 11 isolates was clustered with isolates belonging to PVY^N-Wi strains, suggesting that the 11 PVY isolates shared high homology with PVY^N-Wi strain and accounting for 68.7% of total isolates. 5 PVY isolates were in the same branch with the reference PVY^O strains, indicating 5 PVY isolates were closer to PVY^O. PVY^C, PVY^N, PVY^E and PVY^NTN strains were not detected.

Key words: potato virus Y, cp gene, sequences analysis, maximum likelihood method, Qinghai

CLC Number:

S432.1

CHENG Liang. Analysis on cp gene sequences of potato virus Y from some places in Qinghai Province[J]. , 2019, 31(11): 1888-1895.

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Analysis on cp gene sequences of potato virus Y from some places in Qinghai Province

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