Effects of nitrogen management on soil microbial community structure in paddy fields

doi:10.3969/j.issn.1004-1524.2020.02.15

›› 2020, Vol. 32 ›› Issue (2): 308-316.DOI: 10.3969/j.issn.1004-1524.2020.02.15

• Environmental Science • Previous Articles Next Articles

Effects of nitrogen management on soil microbial community structure in paddy fields

LI Meilin^1,2, CHEN Yutiao³, HONG Xiaofu², QIAO Yuying^2,4, WANG Qingxia², CHEN Xijing², SHEN Alin², YU Man^2,*

1. College of Environment and Resources, Zhejiang A&F University, Hangzhou 311300,China;
2. Institute of Environment, Resource, Soil and Fertilizer, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
3. Institute of Crops and Utilization of Nuclear Technology, Zhejiang Academy of Agricutural Sciences, Hangzhou 310021, China;
4. College of Resources and Environment, Northwest A&F University, Yangling 712100, China

Received:2019-08-30 Online:2020-02-25 Published:2020-03-13

Abstract

Abstract: The microbial communities in four nitrogen fertilizer management modes, namely, zero-nitrogen treatment (CK), farmers’ conventional cultivation pattern (FP), modified farmers’ conventional cultivation pattern 1 (OPT1), and modified farmers’ conventional cultivation pattern 2 (OPT2), were studied by phospholipid fatty acid (PLFA) and Biolog-Eco methods to provide references for the rational utilization of nitrogen fertilizer in paddy fields. It was shown that the microbial metabolic activity and carbon source utilization ability increased in OPT1, while reduced in FP and OPT2 compared to CK. The highest amount of total biomass appeared in OPT1 with 42.11 nmol·g^-1, and was followed by OPT2 with 40.16 nmol·g^-1, which were both higher than that in CK (34.19 nmol·g^-1), but the amount of total biomass in FP was 34.16 nmol·g^-1, which was lower than that in CK. Moreover, the diversity of microbial community in OPT1 was more complex, and F/B value was the highest among the 4 treatments. In summary, the OPT1 treatment was more conducive to the stability of ecological environment in soil.

Key words: paddy, soil microbes, phospholipid fatty acid, nitrogen fertilizer management

CLC Number:

S154.3

LI Meilin, CHEN Yutiao, HONG Xiaofu, QIAO Yuying, WANG Qingxia, CHEN Xijing, SHEN Alin, YU Man. Effects of nitrogen management on soil microbial community structure in paddy fields[J]. , 2020, 32(2): 308-316.

References

[1] 高帅, 潘勇辉, 孙玉明, 等. 不同供氮水平对常规稻与杂交稻产量及氮素利用效率的影响[J]. 南京农业大学学报, 2018, 41(6): 1061-1069.
GAO S, PAN Y H, SUN Y M, et al.Effects of different nitrogen supply on yield and nitrogen utilization of conventional rice and hybrid rice[J]. Journal of Nanjing Agricultural University, 2018, 41(6): 1061-1069.(in Chinese with English abstract)
[2] YOUSAF M, LI X K, ZHANG Z, et al.Nitrogen fertilizer management for enhancing crop productivity and nitrogen use efficiency in a rice-oilseed rape rotation system in China[J]. Frontiers in Plant Science, 2016, 7: 1496.
[3] JU X T, XING G X, CHEN X P, et al.Reducing environmental risk by improving N management in intensive Chinese agricultural systems[J]. Proceedings of the National Academy of Sciences of the United States of America, 2009, 106(9): 3041-3046.
[4] ZHOU Z C, GAN Z T, SHANGGUAN Z P, et al.Effects of long-term repeated mineral and organic fertilizer applications on soil organic carbon and total nitrogen in a semi-arid cropland[J]. European Journal of Agronomy, 2013, 45: 20-26.
[5] 韦应莉, 曹文侠, 李建宏, 等. 不同放牧与围封高寒灌丛草地土壤微生物群落结构PLFA分析[J]. 生态学报, 2018, 38(13): 4897-4908.
WEI Y L, CAO W X, LI J H, et al.Phospholipidfatty acid (PLFA) analysis of soil microbial community structure with different intensities of grazing and fencing in alpine shrubland[J]. Acta Ecologica Sinica, 2018, 38(13): 4897-4908.(in Chinese with English abstract)
[6] 张俊忠. 东祁连山高寒草地土壤微生物生理类群的生态分布及优势真菌的鉴定[D]. 兰州: 甘肃农业大学, 2007.
ZHANG J Z.Ecological distribution of soil microbial physiological groups of alpine grassland in the Eastern Qilian Mountains and identification of the dominant fungi[D]. Lanzhou: Gansu Agricultural University, 2007.(in Chinese with English abstract)
[7] 刘丽君. 浙西(杭州和衢州)低丘土壤发生特性和系统分类研究[D]. 杭州: 浙江大学, 2012.
LIU L J.Genetic characteristics and taxonomic classification of hilly soils in the Western Zhejiang Province[D]. Hangzhou: Zhejiang University, 2012.(in Chinese with English abstract)
[8] 郭莹,王一明,巫攀,等.长期施用粪肥对水稻土中微生物群落功能多样性的影响[J].应用与环境生物学报,2019, 25(3):593-602.
GUO Y, WANG Y M, WU P, et al.Influence of long-term manure application in paddy soil on the functional diversity of microbial community[J]. Chinese Journal of Applied and Environmental Biology, 2019, 25(3): 593-602. (in Chinese with English abstract)
[9] 席劲瑛, 胡洪营, 钱易. Biolog方法在环境微生物群落研究中的应用[J]. 微生物学报, 2003, 43(1): 138-141.
XI J Y, HU H Y, QIAN Y.Application of biolog system in the study of microbial community[J]. Acta Microbiologica Sinica, 2003, 43(1): 138-141.(in Chinese)
[10] CLASSEN A T, BOYLE S I, HASKINS K E, et al.Community-level physiological profiles of bacteria and fungi: plate type and incubation temperature influences on contrasting soils[J]. FEMS Microbiology Ecology, 2003, 44(3): 319-328.
[11] YU M, ZENG G M, CHEN Y N.Microbial communities responsible for the solid-state fermentation of rice straw estimated by phospholipid fatty acid (PLFA) analysis[J]. Process Biochemistry, 2009, 44: 17-22.
[12] 邹立思,马阳,侯娅,等.基于磷脂脂肪酸(PLFA)技术的不同产地太子参根际土壤微生物群落结构多样性分析[J].中药材, 2018, 41(5):1054-1060.
ZOU L S, MA Y, HOU Y, et al.Diversity of microbial community in rhizosphere soil of Pseudostellaria heterophylla in different habitats based on phospholipid fatty acid (PLFA)[J]. Journal of Chinese Medicinal Materials, 2018, 41(5): 1054-1060. (in Chinese with English abstract)
[13] 胡胜华, 蔺庆伟, 代志刚, 等. 西湖沉水植物恢复过程中物种多样性的变化[J]. 生态环境学报, 2018, 27(8): 1440-1445.
HU S H, LIN Q W, DAI Z G, et al.Change of species diversity during the restoration process of submerged macrophyte in Xihu Lake, Hangzhou[J]. Ecology and Environmental Sciences, 2018, 27(8): 1440-1445.(in Chinese with English abstract)
[14] 夏瑜, 何绪文, 文湘华. 微生物群落多样性数学表征方法及其在污水处理系统研究中的应用[J]. 微生物学通报, 2018, 45(8): 1778-1786.
XIA Y, HE X W, WEN X H.Mathematic methods for the evaluation of microbial diversity and their applications in the research on wastewater treatment systems[J]. Microbiology China, 2018, 45(8): 1778-1786.(in Chinese with English abstract)
[15] 贾娟,李硕,高夕彤,等. 氨基酸水溶肥与菌剂配施对松花菜生长及土壤生态特征的作用效果[J]. 河北农业大学学报,2018,41(1):17-23.
JIA J, LI S, GAO X T, et al.Effect of water-soluble fertilizer and microbial agents combined application on the growth of cauliflower and soil ecological characteristics[J]. Journal of Hebei Agricultural University, 2018, 41(1): 17-23. (in Chinese with English abstract)
[16] XUE D, YAO H Y, GE D Y, et al.Soil microbial community structure in diverse land use systems: a comparative study using biolog, DGGE, and PLFA analyses[J]. Pedosphere, 2008, 18(5): 653-663.
[17] 李清华, 王飞, 林诚, 等. 长期施肥对黄泥田土壤微生物群落结构及团聚体组分特征的影响[J]. 植物营养与肥料学报, 2015, 21(6): 1599-1606.
LI Q H, WANG F, LIN C, et al.Effects of long-term fertilization on soil microbial community structure and aggregate composition in yellow clayey paddy field[J]. Journal of Plant Nutrition and Fertilizer, 2015, 21(6): 1599-1606.(in Chinese with English abstract)
[18] 白震, 张明, 宋斗妍, 等. 不同施肥对农田黑土微生物群落的影响[J]. 生态学报, 2008, 28(7): 3244-3253.
BAI Z, ZHANG M, SONG D Y, et al.Effect of different fertilizaiton on microbial community in an arable mollisol[J]. Acta Ecologica Sinica, 2008, 28(7): 3244-3253.(in Chinese with English abstract)
[19] 颜慧, 钟文辉, 李忠佩. 长期施肥对红壤水稻土磷脂脂肪酸特性和酶活性的影响[J]. 应用生态学报, 2008, 19(1): 71-75.
YAN H, ZHONG W H, LI Z P.Effects of long-term fertilization on phospholipid fatty acids and enzyme activities in paddy red soil[J]. Chinese Journal of Applied Ecology, 2008, 19(1): 71-75.(in Chinese with English abstract)
[20] LILJEROTH E, BÅÅTH EE, MATHIASSON EI, et al. Root exudation and rhizoplane bacterial abundance of barley (Hordeum vulgare L.) in relation to nitrogen fertilization and root growth[J]. Plant and Soil, 1990, 127(1): 81-89.
[21] 崔佩佩,武爱莲,王劲松,等.不同施肥处理对高粱根际土壤微生物功能多样性的影响[J].华北农学报,2018,33(5) :195-202.
CUI P P, WU A L, WANG J S, et al.Effect of different fertilization treatments on soil microbial function diversity in rhizosphere of sorghum[J]. Acta Abriculturae Boreall-sinica, 2018, 33(5): 195-202. (in Chinese with English abstract)
[22] 沈荔花, 李碧凉, 任勇杰, 等. OsMYB调控化感水稻酚酸类物质合成及其抑草作用[J]. 作物学报, 2015, 41(4): 531-538.
SHEN L H, LI B L, REN Y J, et al.Phenolic acid synthesis of allelopathic rice regulated by OsMYB and its weed inhibition[J]. Acta Agronomica Sinica, 2015, 41(4): 531-538.(in Chinese with English abstract)
[23] 张奇, 王海斌, 李立, 等. 田间直播下化感水稻抑草作用及其根际土壤微生物生理特性动态[J]. 应用与环境生物学报, 2018, 24(3): 478-482.
ZHANG Q, WANG H B, LI L, et al.Field tests to study the dynamics of weed inhibition of allelopathic rice and microbial physiological traits of rice rhizospheric soils[J]. Chinese Journal of Applied and Environmental Biology, 2018, 24(3): 478-482.(in Chinese with English abstract)
[24] 张俊珍,周迪,郭旭东,等.适度放牧增加内蒙古典型草原甲烷氧化菌丰度和甲烷吸收[J].应用生态学报,2019,30(6):1919-1926.
ZHANG J Z, ZHOU D, GUO X D, et al.Moderate grazing increases the abundance of soil methane-oxidizing bacteria and CH₄ uptake rate in a typical steppe of Inner Mongolia, China[J].Chinese Journal of Applied Ecology,2019,30(6):1919-1926.(in Chinese with English abstract)
[25] 姚先荣. 缓/控释肥料的研究进展及发展趋势[J]. 现代农业科技, 2019(2): 133.
YAO X R.Research progress and development trend of slow/controlled release fertilizer[J]. Modern Agricultural Science and Technology, 2019(2): 133. (in Chinese)
[26] 范国灿. 不同缓控释肥料对水稻秀水134产量和效益的影响[J]. 浙江农业科学, 2018, 59(10): 1785-1787.
FAN G C.Effect of different slow and controlled release fertilizers on yield and benefits of rice Xiushui134[J]. Journal of Zhejiang Agricultural Sciences, 2018, 59(10): 1785-1787.(in Chinese)
[27] 郑圣先, 聂军, 熊金英, 等. 控释肥料提高氮素利用率的作用及对水稻效应的研究[J]. 植物营养与肥料学报, 2001, 7(1): 11-16.
ZHENG S X, NIE J, XIONG J Y, et al.Study on role of controlled release fertilizer in increasing the efficiency of nitrogen utilization and rice yield[J]. Plant Natrition and Fertilizen Science, 2001, 7(1): 11-16.(in Chinese with English abstract)
[28] 周波, 吴海明. 日本缓释尿素在水稻生产上施用效果试验研究[J]. 上海农业科技, 2018(6): 106-107.
ZHOU B, WU H M.Experimental study on the effect of Japanese slow release urea on rice production[J]. Shanghai Agricultural Science and Technology, 2018(6): 106-107.(in Chinese)
[29] 杨斌华,郭桂珍,孙友谊,等.细菌微生物对高分子缓控释化肥降解性能的影响[J].安徽农业科学,2012,40(9):5278-5280.
YANG B H, GUO G Z, SUN Y Y, et al.Effects of microbes on degradation properties of polymer slow controlled-release fertilizer[J]. Journal of Anhui Agricultural Sciences, 2012, 40(9): 5278-5280. (in Chinese with English abstract)

Effects of nitrogen management on soil microbial community structure in paddy fields

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	XIA Wenjian, QIN Wenjing, LIU Jia, CHEN Xiaofen, ZHANG Lifang, CAO Weidong, XU Changxu, CHEN Jingrui. Vertical distribution of soil organic carbon and dissolved organic carbon in reddish paddy soil under long-term green manure utilization [J]. , 2020, 32(5): 878-885.
[2]	PAN Junfeng, ZHONG Xuhua, HUANG Nongrong, LIU Yanzhuo, TIAN Ka, LIANG Kaiming, PENG Bilin, FU Youqiang, HU Xiangyu. Effects of different cultivation patterns on grain yield and nitrogen use efficiency of rice in South China [J]. , 2019, 31(6): 857-868.
[3]	ZHANG Bowei, WANG Haijing, SONG Maoyong, XIE Huijun, ZHANG Jian. Effect of tetrabromobisphenol A on inorganic nitrogen transformation in soil and its possible microbiological mechanism [J]. , 2019, 31(4): 639-645.
[4]	WANG Qingxia, CHEN Xijing, YU Man, SHEN Alin. Research progress on effects of straw returning on nitrogen cycling microbes and functional genes in paddy soil [J]. , 2019, 31(2): 333-342.
[5]	MA Peng, YANG Zhiyuan, LI Yu, LIN Dan, SUN Yongjian, MA Jun. Effects of nitrogen reduction in wheat/rape season and nitrogen fertilizer management in rice season on crop yield and nitrogen uptake in crop rotation system [J]. , 2019, 31(11): 1769-1778.
[6]	MAN Haiyan, HUANG Yunxiang, SHENG Hao, YU Zhan, ZHOU Qing, YUAN Hong, ZHANG Yangzhu. Genetic characteristics and taxonomic classification of paddy field soils developed from two parent materials in East Hunan [J]. , 2018, 30(7): 1194-1201.
[7]	HU Xinyi, FU Qinglin, LIU Chen, DING Nengfei, LIN Yicheng. Effects of straw-returning and tillage depth on soil properties in plough layer of paddy soil [J]. , 2018, 30(7): 1202-1210.
[8]	CHEN Xijing, YU Man, WANG Qiang, LI Hua, SU Yao, GAO Jia, LI Guo'an, LI Jianqiang, SHEN Alin. Problems and countermeasures of straw returning in paddy field system in Zhejiang Province [J]. , 2018, 30(10): 1765-1774.
[9]	CHEN Chongjun, LIU Yuxue, FENG Yu, WANG Jianfang. Effects of bamboo charcoal addition on nitrogen and phosphorus loss and microbial community structures in greenhouse vegetable soil [J]. , 2018, 30(1): 123-128.
[10]	YANG Zifeng, CHEN Weiqiang, WANG Wei, LI Wanyi, HE Xianbiao, YANG Xiangtian. Effect of rotation tillage on soil microbial biomass and enzyme activity of broccoli [J]. , 2017, 29(5): 818-823.
[11]	FENG Yunyu, LUO Longzao, TIAN Guangming. Study on greenhouse vegetables-paddy coupled mode in interception of nitrogen and phosphorus [J]. , 2017, 29(3): 469-476.
[12]	YUAN Da\\|gang， PU Guang\\|lan， CHENG Wei\\|li， WANG Chang\\|quan， HE Gang. Changes in vertical distribution characteristics of soil pH and humus fractions after paddy field switched to tea garden in west Sichuan Basin [J]. , 2016, 28(1): 104-.
[13]	LYU Hao\\|hao1，2， LIU Yu\\|xue1，2， YANG Sheng\\|mao1，2，*， ZOU Ping1， LIU Guang\\|rong3， XU Chang\\|xu3. Classification comparison and improvement techniques of cold waterlogged paddy in Southern District， China [J]. , 2015, 27(5): 822-.
[14]	TU Wen\\|wen1，SONG Zhao\\|liang1，WANG Xu\\|dong1， YE Zheng\\|qian1， LI Zi\\|min2，SUN Ying1. Effects of mixing fertilization of silicon and phosphorus on fractionation and availability of phosphorus in a paddy soil [J]. , 2014, 26(6): 1596-.
[15]	JI Xiaojiang;CHEN Yi;WU Chunyan;LI Chaoying;LI Yan;TANG Xu*. Current status and evolution trends of soil nutrients in the paddy soils in Zhejiang Province [J]. , 2014, 26(3): 0-775778.