Research progress on effects of straw returning on nitrogen cycling microbes and functional genes in paddy soil

doi:10.3969/j.issn.1004-1524.2019.02.20

›› 2019, Vol. 31 ›› Issue (2): 333-342.DOI: 10.3969/j.issn.1004-1524.2019.02.20

• Review • Previous Articles

Research progress on effects of straw returning on nitrogen cycling microbes and functional genes in paddy soil

WANG Qingxia^{1, 2}, CHEN Xijing², YU Man^{2, *}, SHEN Alin^{2, *}

1. College of Environment and Resources, Zhejiang A&F University, Hangzhou 311300, China;;
2. Institute of Environment, Resource, Soil and Fertilizer, Zhejiang Academy of Agricultural Sciences, Hangzhou 310016, China

Received:2018-07-19 Online:2019-02-25 Published:2019-03-06

Abstract

Abstract: The response of soil microorganisms to soil changes is extremely sensitive, and the impact of straw returning on soil microbial community is important for the soil nitrogen cycling maintainance in paddy field systems. Straw incorporation to soils influences the biochemical transformation of soil nitrogen by changing the soil microecological environment. The nitrogen management strategy in rice production is closely related to these changes in soil nitrogen transformation. Based on the latest published research papers, this study reviewed the soil nitrogen transformation processes, the functions of related functional microbes, and the driving mechanisms of the key functional genes in paddy field soil with straw incorporation. It would help to understand the microbial mechanism of soil nitrogen cycling in paddy field soils with straw incorporation, and provide theoretical basis for the scientific application of nitrogen fertilizer in paddy field systems.

Key words: straw returning, paddy field, nitrogen cycle, soil microorganisms, functional genes

CLC Number:

S154
S153

WANG Qingxia, CHEN Xijing, YU Man, SHEN Alin. Research progress on effects of straw returning on nitrogen cycling microbes and functional genes in paddy soil[J]. , 2019, 31(2): 333-342.

References

[1] 杨丽, 刘文, 兰韬, 等. 我国秸秆还田技术与标准的现状研究[J]. 中国农业信息, 2017 (21): 12-17.
YANG L, LIU W, LAN T, et al.Research on the status quo of technology and standard of straw returning in China[J]. China Agricultural Information, 2017 (21): 12-17. (in Chinese)
[2] 胡乃娟, 韩新忠, 杨敏芳, 等. 秸秆还田对稻麦轮作农田活性有机碳组分含量、酶活性及产量的短期效应[J]. 植物营养与肥料学报, 2015, 21(2): 371-377.
HU N J, HAN X Z, YANG M F, et al.Short-term influence of straw return on the contents of soil organic carbon fractions,enzyme activities and crop yields in rice-wheat rotation farmland[J]. Journal of Plant Nutrition and Fertilizer, 2015, 21(2): 371-377. (in Chinese with English abstract)
[3] 董印丽, 李振峰, 王若伦, 等. 华北地区小麦、玉米两季秸秆还田存在问题及对策研究[J]. 中国土壤与肥料, 2018 (1): 159-163.
DONG Y L, LI Z F, WANG R L, et al.Study on the problems and countermeasures of returning wheat and corn stalks into the North China[J]. Soil and Fertilizer Sciences in China, 2018 (1): 159-163. (in Chinese with English abstract)
[4] 何文寿, 李生秀, 李辉桃. 水稻对铵态氮和硝态氮吸收特性的研究[J]. 中国水稻科学, 1998, 12(4): 249-252.
HE W S, LI S X, LI H T.Characteristics of ammonium and nitrate uptake in rice[J]. Chinese Journal of Rice Science, 1998, 12(4): 249-252. (in Chinese with English abstract)
[5] 赵士诚, 曹彩云, 李科江, 等. 长期秸秆还田对华北潮土肥力、氮库组分及作物产量的影响[J]. 植物营养与肥料学报, 2014, 20(6): 1441-1449.
ZHAO S C, CAO C Y, LI K J, et al.Effects of long-term straw return on soil fertility, nitrogen pool fractions and crop yields on a fiuvo-aquic soil in North China[J]. Journal of Plant Nutrition and Fertilizer, 2014, 20(6): 1441-1449. (in Chinese with English abstract)
[6] LIANG B, YANG X Y, HE X H, et al.Long-term combined application of manure and NPK fertilizers influenced nitrogen retention and stabilization of organic C in loess soil[J]. Plant and Soil, 2012, 353: 249-260.
[7] TAKAHASHI S, UENOSONO S, ONO S.Short-and long-term effects of rice straw application on nitrogen uptake by crops and nitrogen mineralization under flooded and upland conditions[J]. Plant and Soil, 2003, 251(2): 291-301.
[8] CAO Y S, SUN H F, ZHANG J N, et al.Effects of wheat straw addition on dynamics and fate of nitrogen applied to paddy soils[J]. Soil & Tillage Research, 2018, 178: 92-98.
[9] 张聪, 慕平, 尚建明. 长期持续秸秆还田对土壤理化特性、酶活性和产量性状的影响[J]. 水土保持研究, 2018, 25(1): 92-98.
ZHANG C, MU P, SHANG J M.Effects of continuous returning corn straw on soil chemical properties, enzyme activities and yield trait[J]. Research of Soil and Water Conservation, 2018, 25(1): 92-98. (in Chinese with English abstract)
[10] 张武益. 灌溉和秸秆还田对水稻产量及土壤氧化还原特性的影响[D]. 南京: 南京农业大学, 2014.
ZHANG W Y.Effect of irrigation and wheat straw returning on rice yield and soil oxidation-reduction[D]. Nanjing: Nanjing Agricultural University, 2014. (in Chinese with English abstract)
[11] 陈贵, 陈莹, 施卫明. 氮肥对水稻不同生长期土壤不同深度氮素渗漏的影响[J]. 土壤, 2013, 45(5): 809-814.
CHEN G, CHEN Y, SHI W M.Effect of fertilizer N on N leaching at different soil depths during growth periods of rice[J]. Soils, 2013, 45(5): 809-814. (in Chinese with English abstract)
[12] 陶爽, 华晓雨, 王英男, 等. 不同氮素形态对植物生长与生理影响的研究进展[J]. 贵州农业科学, 2017, 45(12): 64-68.
TAO S, HUA X Y, WANG Y N, et al.Research advance in effects of different nitrogen forms on growth and physiology of plants[J]. Guizhou Agricultural Sciences, 2017, 45(12): 64-68. (in Chinese with English abstract)
[13] ZHAO Y, PANG H, WANG J, et al.Effects of straw mulch and buried straw on soil moisture and salinity in relation to sunflower growth and yield[J]. Field Crops Research, 2014, 161: 16-25.
[14] 张莉, 王婧, 逄焕成, 等.短期秸秆颗粒还田对小麦-玉米系统作物产量与土壤呼吸的影响[J]. 应用生态学报, 2018, 29(2): 565-572.
ZHANG L, WANG J, PANG H C, et al.Effects of short-term granulated straw incorporation on grain yield and soil respiration in a winter wheat-summer maize cropping system[J]. Chinese Journal of Applied Ecology, 2018, 29(2): 565-572. (in Chinese with English abstract)
[15] 王宁, 于建光, 常志州, 等. 稻田土壤真菌群落多样性和组成对麦秸还田的响应[J]. 土壤, 2017, 49(6): 1115-1120.
WANG N, YU J G, CHANG Z Z, et al.Responses of fungal community diversity and composition in paddy soils to straw return[J]. Soils, 2017, 49(6): 1115-1120. (in Chinese with English abstract)
[16] 舒丽. 秸秆还田不同耕作方式对水稻土微生物特性的影响[D]. 成都: 四川农业大学, 2008.
SHU L.Effect of straw returning under different tillage patterns on soil microbiological properties[D]. Chengdu: Sichuan Agricultural University, 2008. (in Chinese with English abstract)
[17] 刘杰. 保护性耕作条件下黄绵土有机质含量变化及其生物学机制[D]. 兰州: 甘肃农业大学, 2016.
LIU J.Changes in soil organic matter and biological mechanisms under conservation tillage conditions[D]. Lanzhou: Gansu Agricultural University, 2016. (in Chinese with English abstract)
[18] 于寒, 梁烜赫, 张玉秋, 等. 不同秸秆还田方式对玉米根际土壤微生物及酶活性的影响[J]. 农业资源与环境学报, 2015, 32(3): 305-311.
YU H, LIANG X H, ZHANG Y Q, et al.Effects of different straw returning modes on the soil microorganism and enzyme activity in corn field[J]. Journal of Agricultural Resources and Environment, 2015, 32(3): 305-311. (in Chinese with English abstract)
[19] ZHAO S C, LI K J, ZHOU W, et al.Changes in soil microbial community, enzyme activities and organic matter fractions under long-term straw return in north-central China[J]. Agriculture, Ecosystems and Environment, 2016, 216: 82-88.
[20] 张婷, 张一新, 向红勇. 秸秆还田培肥土壤的效应及机制研究进展[J]. 江苏农业科学, 2018, 46(3): 14-20.
ZHANG T, ZHANG Y X, XIANG H Y.Research progress on effect and mechanism of straw returning to soil[J]. Jiangsu Agricultural Sciences, 2018, 46(3): 14-20. (in Chinese)
[21] 杨宪龙, 路永莉, 同延安, 等. 长期施氮和秸秆还田对小麦-玉米轮作体系土壤氮素平衡的影响[J]. 植物营养与肥料学报, 2013, 19(1): 65-73.
YANG X L, LU Y L, TONG Y A, et al.Effects of long-term N application and straw returning on N budget under wheat-maize rotation system[J]. Journal of Plant Nutrition and Fertilizer, 2013, 19(1): 65-73. (in Chinese with English abstract)
[22] 杨雪琴, 连英丽, 颜庆云, 等.滨海湿地生态系统微生物驱动的氮循环研究进展[J]. 微生物学报, 2018, 58(4): 633-648.
YANG X Q, LIAN Y L, YAN Q Y, et al.Microbially-driven nitrogen cycling in coastal ecosystems[J]. Acta Microbiologica Sinica, 2018, 58(4):633-648. (in Chinese with English abstract)
[23] CHE R X, QIN J L, TAHMASBIAN I, et al.Litter amendment rather than phosphorus can dramatically change inorganic nitrogen pools in a degraded grassland soil by affecting nitrogen-cycling microbes[J]. Soil Biology & Biochemistry, 2018, 120: 145-152.
[24] 韩新忠, 朱利群, 杨敏芳, 等. 不同小麦秸秆还田量对水稻生长、土壤微生物生物量及酶活性的影响[J]. 农业环境科学学报, 2012, 31(11): 2192-2199.
HAN X Z, ZHU L Q, YANG M F, et al.Effects of different amount of wheat straw returning on rice growth, soil microbial biomass and enzyme activity[J]. Journal of Agro-Environment Science, 2012, 31(11): 2192-2199. (in Chinese with English abstract)
[25] 王前, 陈海魁, 王俊丽. 固氮基因的分子进化分析[J]. 江西农业大学学报, 2013, 35(3): 597-602.
WANG Q,CHEN H K,WANG J L.An analysis of molecular evolution of nitrogen fixation genes[J]. Acta Agriculturae Universitatis Jiangxiensis, 2013, 35(3): 597-602. (in Chinese with English abstract)
[26] 康文龙, 台喜生, 李师翁, 等. 祁连山高寒草原碱性土壤固氮微生物数量及固氮基因(nifH)群落结构研究[J]. 冰川冻土, 2013, 35(1): 208-216.
KANG W L, TAI X S, LI S W, et al.Research on the number of nitrogen-fixing microorganism and community structure of nitrogen-fixing (nifH) genes in the alkali soils of alpine steppe in the Qilian Mountains[J]. Journal of Glaciology and Geocryology, 2013, 35(1): 208-216. (in Chinese with English abstract)
[27] 刘骁蒨, 涂仕华, 孙锡发, 等. 秸秆还田与施肥对稻田土壤微生物生物量及固氮菌群落结构的影响[J]. 生态学报, 2013, 33(17): 5210-5218.
LIU X Q, TU S H, SUN X F, et al.Effect of different fertilizer combinations and straw return on microbial biomass and nitrogen fixing bacteria community in a paddy soil[J]. Acta Ecologica Sinica, 2013, 33(17): 5210-5218. (in Chinese with English abstract)
[28] 杨滨娟, 黄国勤, 钱海燕. 秸秆还田配施化肥对土壤温度、根际微生物及酶活性的影响[J]. 土壤学报, 2014, 51(1): 150-157.
YANG B J, HUANG G Q, QIAN H Y.Effects of straw incorporation plus chemical fertilizer on soil temperature, root micro-organisms and enzyme activities[J]. Acta Pedologica Sinica, 2014, 51(1): 150-157. (in Chinese with English abstract)
[29] TANG Y F, ZHANG M M, CHEN A L, et al.Impact of fertilization regimes on diazotroph community compositions and N₂-fixation activity in paddy soil[J]. Agriculture, Ecosystems and Environment, 2017, 247: 1-8.
[30] PEREG L, MORUGÁN-CORONADO A, MCMILLAN M, et al. Restoration of nitrogen cycling community in grapevine soil by a decade of organic fertilization[J]. Soil and Tillage Research, 2018, 179: 11-19.
[31] 李奕林. 水稻根际通气组织与根系泌氧及根际硝化作用的关系[J]. 生态学报, 2012, 32(7): 2066-2074.
LI Y L.Relationship among rice root aerechyma, root radial oxygen loss and rhizosphere nitrification[J]. Acta Ecologica Sinica, 2012, 32(7): 2066-2074. (in Chinese with English abstract)
[32] 王沛譞, 徐焱, 宋亚娜. 转基因水稻秸秆还田对土壤硝化反硝化微生物群落的影响[J]. 中国生态农业学报, 2018, 26(1): 8-15.
WANG P X, XU Y, SONG Y N.Effect of transgenic rice straw return to soil on nitrification and denitrification microbial community[J]. Chinese Journal of Eco-Agriculture, 2018, 26(1): 8-15. (in Chinese with English abstract)
[33] BAO Q L, JU X T, GAO B, et al.Response of nitrous oxide and corresponding bacteria to managements in an agricultural soil[J]. Soil Science Society of America Journal, 2012, 76(1): 130-141.
[34] 郑燕, 侯海军, 秦红灵, 等. 施氮对水稻土N₂O释放及反硝化功能基因(narG/nosZ)丰度的影响[J]. 生态学报, 2012, 32(11): 3386-3393.
ZHENG Y, HOU H J, QIN H L, et al.Effect of N application on the abundance of denitrifying genes (narG/nosZ) and N₂O emission in paddy soil[J]. Acta Ecologica Sinica, 2012, 32(11): 3386-3393. (in Chinese with English abstract)
[35] BAOLAN H, SHUAI L, LIDONG S, et al.Effect of different ammonia concentrations on community succession of ammonia-oxidizing microorganisms in a simulated paddy soil column[J]. PLoS One, 2012, 7(8): e44122.
[36] 陈春兰, 陈哲, 朱亦君, 等. 水稻土细菌硝化作用基因(amoA和hao)多样性组成与长期稻草还田的关系研究[J]. 环境科学, 2010, 31(6): 1624-1632.
CHEN C L, CHEN Z, ZHU Y J, et al.Effects of rice straw on the diversity of nitrifying genes (amoA and hao) in paddy soil[J]. Environmental Science, 2010, 31(6): 1624-1632. (in Chinese with English abstract)
[37] JIN Z J, LI L Q, LIU X Y, et al.Impact of long-term fertilization on community structure of ammonia oxidizing and denitrifying bacteria based on amoA and nirK genes in a rice paddy from Tai Lake Region, China[J]. Journal of Integrative Agriculture, 2014, 13(10): 2286-2298.
[38] SUN X, HAN X G, PING F, et al. Effect of rice-straw biochar on nitrous oxide emissions from paddy soils under elevated CO₂ and temperature[J]. Science of the Total Environment, 2018, 628/629: 1009-1016.
[39] LIN Y X, DING W X, LIU D Y, et al.Wheat straw-derived biochar amendment stimulated N₂O emissions from rice paddy soils by regulating the amoA genes of ammonia-oxidizing bacteria[J]. Soil Biology & Biochemistry, 2017, 113: 89-98.
[40] ABID A A, GU C, ZHANG Q, et al.Nitrous oxide fluxes and nitrifier and denitrifier communities as affected by dry-wet cycles in long term fertilized paddy soils[J]. Applied Soil Ecology, 2018, 125: 81-87.
[41] 蔚霞. 转Bt基因水稻秸秆还田对水稻土反硝化作用和微生物多样性的影响[D]. 武汉: 华中农业大学, 2012.
YU X.Effect of transgenic Bt gene rice straw on denitrification and microbial diversity in paddy soil[D]. Wuhan: Huazhong Agricultural University, 2012. (in Chinese with English abstract)
[42] WANG N, YU J G, ZHAO Y H, et al.Straw enhanced CO₂ and CH₄ but decreased N₂O emissions from flooded paddy soils: changes in microbial community compositions[J]. Atmospheric Environment, 2018, 174: 171-179.
[43] BENDER S F, PLANTENGA F, NEFTEL A, et al.Symbiotic relationships between soil fungi and plants reduce N₂O emissions from soil[J]. The ISME Journal, 2014, 8(6): 1336-1345.
[44] AZZIZ G, MONZA J, ETCHEBEHERE C, et al.nirS-and nirK-type denitrifier communities are differentially affected by soil type, rice cultivar and water management[J]. European Journal of Soil Biology, 2017, 78: 20-28.
[45] WANG L, SHENG R, YANG H C, et al.Stimulatory effect of exogenous nitrate on soil denitrifiers and denitrifying activities in submerged paddy soil[J]. Geoderma, 2017, 286: 64-72.
[46] CUCU M A, MARHAN S, SAID-PULLICINO D, et al.Resource driven community dynamics of N$H⁺₄$ assimilating and N₂O reducing archaea in a temperate paddy soil[J]. Pedobiologia, 2017, 62: 16-27.
[47] 王改玲, 李立科, 郝明德. 长期施肥和秸秆覆盖土壤活性有机质及碳库管理指数变化[J]. 植物营养与肥料学报, 2017, 23(1): 20-26.
WANG G L, LI L K, HAO M D.Effect of long-term fertilization and straw mulch on the contents of labile organic matter and carbon management index[J]. Journal of Plant Nutrition and Fertilizer, 2017, 23(1): 20-26. (in Chinese with English abstract)
[48] 程吕. 上海城市河网沉积物硝酸异化还原过程及其环境影响因素研究[D]. 上海: 华东师范大学, 2017.
CHENG L.Dissimilatory nitrate reduction processes and associated influencing factors in the sediments of Shanghai urban river networks[D]. Shanghai: East China Normal University, 2017. (in Chinese with English abstract)
[49] YIN S X, CHEN D, CHEN L M, et al.Dissimilatory nitrate reduction to ammonium and responsible microorganisms in two Chinese and Australian paddy soils[J]. Soil Biology & Biochemistry, 2002, 34(8): 1131-1137.
[50] WOODS D D.The reduction of nitrate to ammonia by Clostridium welchii[J]. Biochemical Journal, 1938, 32(11): 2000-2012.
[51] STANFORD G, LEGG J O, DZIENIA S, et al.Denitrification and associated nitrogen transformations in soils[J]. Soil Science, 1975, 120(2): 147-152.
[52] ZHANG C B, LIU W L, HAN W J, et al.Responses of dissimilatory nitrate reduction to ammonium and denitrification to plant presence, plant species and species richness in simulated vertical flow constructed wetlands[J]. Wetlands, 2017, 37(1): 109-122.
[53] LU W W, RIYA S, ZHOU S, et al.In situ dissimilatory nitrate reduction to ammonium in a paddy soil fertilized with liquid cattle waste[J]. Pedosphere, 2012, 22(3): 314-321.
[54] LU W W, ZHANG H L, SHI W M.Dissimilatory nitrate reduction to ammonium in an anaerobic agricultural soil as affected by glucose and free sulfide[J]. European Journal of Soil Biology, 2013, 58: 98-104.
[55] 陈韬, 李剑沣, 邹子介, 等. 氧化还原电位和pH对生物滞留系统硝酸盐异化还原为氨作用的影响[J]. 科学技术与工程, 2018, 18(4): 368-373.
CHEN T, LI J F, ZOU Z J, et al.Effects of redox potential and pH on the effect of dissimilatory nitrate reduction to ammonium in bioretention system[J]. Science Technology and Engineering, 2018, 18(4): 368-373. (in Chinese with English abstract)
[56] ZHANG J B, LAN T, MÜLLER C, et al. Dissimilatory nitrate reduction to ammonium (DNRA) plays an important role in soil nitrogen conservation in neutral and alkaline but not acidic rice soil[J]. Journal of Soils and Sediments, 2015, 15(3): 523-531.
[57] SCHMIDT C S, RICHARDSON D J, BAGGS E M.Constraining the conditions conducive to dissimilatory nitrate reduction to ammonium in temperate arable soils[J]. Soil Biology & Biochemistry, 2011, 43(7): 1607-1611.
[58] TATTI E, GOYER C, ZEBARTH B J, et al.Over-winter dynamics of soil bacterial denitrifiers and nitrite ammonifiers influenced by crop residues with different carbon to nitrogen ratios[J]. Applied Soil Ecology, 2017, 110: 53-64.
[59] LU W W, ZHANG H L, MIN J, et al.Dissimilatory nitrate reduction to ammonium in a soil under greenhouse vegetable cultivation as affected by organic amendments[J]. Journal of Soils & Sediments, 2015, 15(5): 1169-1177.
[60] WANG J, ZHU B, ZHANG J B, et al.Mechanisms of soil N dynamics following long-term application of organic fertilizers to subtropical rain-fed purple soil in China[J]. Soil Biology & Biochemistry, 2015, 91: 222-231.
[61] JAHANGIR M M R, FENTON O, MÜLLER C, et al. In situ denitrification and DNRA rates in groundwater beneath an integrated constructed wetland[J]. Water Research, 2017, 111: 254-264.
[62] RUBOL S, MANZONI S, BELLIN A, et al.Modeling soil moisture and oxygen effects on soil biogeochemical cycles including dissimilatory nitrate reduction to ammonium (DNRA)[J]. Advances in Water Resources, 2013, 62: 106-124.

Research progress on effects of straw returning on nitrogen cycling microbes and functional genes in paddy soil

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 13

Recommended Articles

Metrics

Comments

[1]	WANG Baojun, CHENG Wangda, CHEN Gui, SHEN Yaqiang, SHEN Meng, YUAN Ye, WANG Lei, ZHANG Hongmei. Effects of nitrogen fertilizer regulation on soil properties of paddy fields and rice yield with full amount returning of straw in Northern Zhejiang [J]. , 2020, 32(2): 183-190.
[2]	WANG Baojun, CHENG Wangda, CHEN Gui, SHEN Yaqiang, ZHANG Hongmei. Effect of straw returning and nitrogen reduction on soil nutrition, carbon pool and rice yield in rice field [J]. , 2019, 31(4): 624-630.
[3]	GOU Liqiong, YAO Heng, WANG Ge, HUAGN Rucheng, DUAN Junhua, XIAO Jiujin, ZHANG Jian. Effects of different straw returning methods on cropland soil fauna community [J]. , 2019, 31(3): 450-457.
[4]	MAN Haiyan, HUANG Yunxiang, SHENG Hao, YU Zhan, ZHOU Qing, YUAN Hong, ZHANG Yangzhu. Genetic characteristics and taxonomic classification of paddy field soils developed from two parent materials in East Hunan [J]. , 2018, 30(7): 1194-1201.
[5]	LIANG Jingang, JIAO Yue, LIU Pengcheng, ZHANG Xiujie. Arbuscular mycorrhizal fungi as a potential indicator to assess effects of genetically modified crops on soil microorganisms [J]. , 2018, 30(7): 1267-1272.
[6]	SA Rula, YANG Hengshan, GAO Julin, FAN Fu, ZHANG Ruifu, LIU Jing, WU Shuai. Effects of maize straw returning modes on soil fertility and maize yield [J]. , 2018, 30(2): 268-274.
[7]	CHEN Xijing, YU Man, WANG Qiang, LI Hua, SU Yao, GAO Jia, LI Guo'an, LI Jianqiang, SHEN Alin. Problems and countermeasures of straw returning in paddy field system in Zhejiang Province [J]. , 2018, 30(10): 1765-1774.
[8]	GUO Jing, ZHOU Kejin, LIU Fang, CHEN Lin, ZHA Daogui. Effects of returning amount and manner of wheat straw on maize growth in lime concretion black soil field [J]. , 2017, 29(4): 521-527.
[9]	YUAN Da\\|gang， PU Guang\\|lan， CHENG Wei\\|li， WANG Chang\\|quan， HE Gang. Changes in vertical distribution characteristics of soil pH and humus fractions after paddy field switched to tea garden in west Sichuan Basin [J]. , 2016, 28(1): 104-.
[10]	AO Jin\\|cheng1， LUO Hua\\|yuan2， ZHANG Xiao\\|long1， CHEN Chu2， MAO Chun\\|tang1， LYU Kai1， LI Wei\\|bao3， ZI Wen\\|hua1，*. Effects of different corn straw returning modes on quality of flue\\|cured tobacco leaves and soil fertility#br# [J]. , 2015, 27(8): 1456-.
[11]	LI Qing\\|hai1，ZHU Rong2，LOU Li\\|feng1，LIU Rong\\|ping2，WANG Huan\\|huan1，XIA Zhou\\|wu2，ZHANG Xue\\|dong1，*. The polymorphisms of five functional genes in Chunan spotted pig [J]. , 2015, 27(2): 171-.
[12]	LIU Jun;TAN Ji\\|cai;*;ZHONG Lang;WANG Zhi\\|gao. Evaluation of several agricultural practices in preventing the apple snails， Pomacea canaliculata， in the paddy fields [J]. , 2013, 25(6): 0-1325.
[13]	BAI Peng\\|hua;LIU Qi\\|zhi;*;LI Hong\\|xyu;LIU Yan\\|bin;SHI Wang\\|peng;WANG Wei;LIU Zhi\\|long;ZHOU Hai\\|ying;ZHAO Ming\\|xin. Relationships between leaf yellowing and soil microorganisms/nutrients in pear orchards [J]. , 2013, 25(6): 0-1336.