Effect of exogenous proline on degradation of residual chlorothalonil in tomato

doi:10.3969/j.issn.1004-1524.2020.03.08

›› 2020, Vol. 32 ›› Issue (3): 437-446.DOI: 10.3969/j.issn.1004-1524.2020.03.08

• Horticultural Science • Previous Articles Next Articles

Effect of exogenous proline on degradation of residual chlorothalonil in tomato

LIU Xinyu¹, CHEN Peng², ZHANG Guanghui¹, ZHAO Junjie¹, LI Bohao¹, ZHANG Nan¹, SUN Weike¹, XU Juanjuan¹, YE Xingtao¹, WEI Jinpeng¹, YU Gaobo^1,*

1.Heilongjiang Bayi Agricultural University, Daqing 163319, China;
2.Agricultural College, Guangxi University, Nanning 530001,China

Received:2019-08-20 Online:2020-03-25 Published:2020-04-03

Abstract

Abstract: In order to explore the regulation of exogenous proline (Pro) on degradation of residual pesticide in tomato,Zheza 205 was taken as the tested plant and the fungicide chlorothalonil was applied to study the effect of exogenous Pro on pesticide residues. The content of reactive oxygen species(ROS),and the activity of antioxidant enzymes and detoxification enzymes after the application of chlorothalonil were investigated. The results showed that pretreatment of Pro (3 mmol·L^-1) reduced the pesticide residue in tomato significantly after 7 days of chlorothalonil application. Compared with the control,exogenous Pro pretreatment could induce the activity of tomato peroxidase (POD), superoxide dismutase (SOD), ascorbate peroxidase (APX), monodehydroascorbic acid reductase (MDAR) significantly after the treatment of chlorothalonil, and increase the GSH/GSSG ratio during the 12-96 h period after pesticide treatment, as well as the activity of glutathione reductase (GR), glutathione S-transferase (GST), and the content of non protein bound thiols (NPT).Therefore, pretreatment of Pro could induce the activity of antioxidant enzymes and GST detoxification enzyme and improve the regeneration of GSH, and thus promote the degradation and metabolism of chlorothalonil in tomato.This study provided a new way to promote the degradation of pesticide residue in vegetables.

Key words: tomato, proline, chlorothalonil, antioxidant enzyme, detoxification enzyme

CLC Number:

S641.2

LIU Xinyu, CHEN Peng, ZHANG Guanghui, ZHAO Junjie, LI Bohao, ZHANG Nan, SUN Weike, XU Juanjuan, YE Xingtao, WEI Jinpeng, YU Gaobo. Effect of exogenous proline on degradation of residual chlorothalonil in tomato[J]. , 2020, 32(3): 437-446.

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Effect of exogenous proline on degradation of residual chlorothalonil in tomato

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