Optimization of total triterpenoids extraction from Ganoderma lucidum spore powder and evaluations of their biological capacities in vitro

doi:10.3969/j.issn.1004-1524.2020.04.18

›› 2020, Vol. 32 ›› Issue (4): 705-713.DOI: 10.3969/j.issn.1004-1524.2020.04.18

• Food Science • Previous Articles Next Articles

Optimization of total triterpenoids extraction from Ganoderma lucidum spore powder and evaluations of their biological capacities in vitro

WEN Huiping, LEI Weimin, KE Leqin, CHEN Yuwei, HOU Jing, XIAO Jianzhong^*

School of Ecology, Lishui University, Lishui 323000, China

Received:2019-09-10 Online:2020-04-25 Published:2020-04-26

Abstract

Abstract: To optimize the ultrasonic extraction technology of total triterpenoids from Ganoderma lucidum spore powder, and investigate its antioxidant and antitumor activity in vitro, the extraction method was optimized to achieve high yield. The significant influencing factors were firstly screened by Plackett-Burman (PB) design on the basis of single factor experiment, and then were further optimized by Box-Behnken. Antioxidant activity of total triterpenoids in vitro was evaluated by DPPH and ABTS⁺ free radical scavenging activity assay and the effect of total triterpenoids on the human hepatoma HepG-2 cells was detected by CCK-8. The results showed that the optimal extraction conditions were as follows: ethanol volume fraction of 80%, solid-liquid ratio of 1:23, ultrasonic power of 540 W, ultrasonic temperature of 40 ℃, ultrasonic time of 60 min. The bioactivity studies showed that the total triterpenoids extracted from Ganoderma lucidum spore powder had better ability to scavenge ABTS⁺ than DPPH free radicals, and had anti-proliferation activity against human hepatoma cell (HepG-2).

Key words: total triterpenoids, optimal extraction, antioxidation

CLC Number:

TS201.1

WEN Huiping, LEI Weimin, KE Leqin, CHEN Yuwei, HOU Jing, XIAO Jianzhong. Optimization of total triterpenoids extraction from Ganoderma lucidum spore powder and evaluations of their biological capacities in vitro[J]. , 2020, 32(4): 705-713.

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Optimization of total triterpenoids extraction from Ganoderma lucidum spore powder and evaluations of their biological capacities in vitro

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