›› 2020, Vol. 32 ›› Issue (10): 1764-1771.DOI: 10.3969/j.issn.1004-1524.2020.10.04

• Animal Science • Previous Articles     Next Articles

Effects of early weaning and dietary glutamine addition on serum free amino acids and their metabolites in piglets

ZHAO Guangmin1,2, LIU Xiuting1, DAI Bing2, YANG Hua1, LYU Wentao1, WANG Yuanxia1, XIAO Yingping1,*   

  1. 1. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Quality and Standard for Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
    2. College of Animal Science, Zhejiang A&F University, Hangzhou 311300, China
  • Received:2020-06-04 Online:2020-10-25 Published:2020-11-16

Abstract: To study the effects of early weaning and glutamine supplementation on free amino acids and metabolites in serum of piglets, suckling, weaning and glutamine feeding sows were selected for the experiment. The growth performance and amino acid content of piglets were analyzed by chromatographic analysis, principal component analysis and partial least square discriminant analysis. The results showed that the content of arginine, histidine, valine, cysteine, proline, serine and tyrosine in the weaning group was significantly lower than that in the lactation group (P<0.05), and the contents of isoleucine, methionine, phenylmethyl-alanine, threonine and alanine in weaning group were significantly higher than those in lactation group (P<0.05). The contents of threonine and glycine in glutamine group were significantly lower than that in weaning group (P<0.05), and the contents of leucine, isoleucine, cysteine and valine in glutamine group were significantly higher than that in weaning group (P<0.05). Among amino acid metabolites, the content of γ-aminobutyric acid, taurine, citrulline, carnosine, ethanolamine and hydroxylysine in weaning group was significantly lower than that in lactation group (P<0.05). and the content of α-aminophenic acid, cystathionine, anserine, 1-methylhistidine, 3-methylhistidine, phosphoethanolamine, phosphoserine and urea in the weaning group was significantly higher than that in the lactating group (P<0.05), and the contents of ornithine, anserine and phosphorylethanolamine in the glutamine group were significantly lower than those in the weaning group (P<0.05), and the contents of α-aminophenic acid, α-aminobutyric acid, carnosine, ethanolamine, hydroxylysine, serine phosphate and urea in the glutamine group were significantly higher than those in the weaning group (P<0.05). The results showed that proper feeding of glutamine could help free amino acids and their metabolites to return to normal level. Principal component analysis showed that there were significant differences in metabolite phenotypes among the three test groups (P<0.05). The partial least square discriminant analysis model showed that threonine, valine, cysteine, anserine, phosphoethanolamine, ethanolamine and hydroxylysine were important amino acids and their metabolites. It can be seen that the content of amino acids in serum of piglets changes significantly in the early weaning stage, and the addition of glutamine can obviously regulate the content of free amino acids in the serum of piglets.

Key words: weaned piglets, glutamate, free amino acids, early weaning

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