Structures and characteristics of 103 plasmids carrying mcr gene

doi:10.3969/j.issn.1004-1524.2021.01.06

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (1): 43-51.DOI: 10.3969/j.issn.1004-1524.2021.01.06

• Animal Science • Previous Articles Next Articles

Structures and characteristics of 103 plasmids carrying mcr gene

TANG Biao^1a^,^1b(), CHANG Jiang^1a^,^1b^,², HU Ji^1b, QIAN Mingrong^1a^,^1b, XIA Xiaodong³, YANG Hua^1a^,^1b^,^*()

1. a State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products (in Prepared), b Institute of Quality and Standard for Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
2. School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China
3. College of Food Science and Engineering, Northwest A & F University, Yangling 712100, China

Received:2020-07-31 Online:2021-01-25 Published:2021-01-25
Contact: YANG Hua

Abstract

Abstract:

Colistin is the last first-line drug in the treatment of carbapenem-resistant pathogens infection. However, the widespread of plasmid mediated mcr gene which involved colistin resistance had reduced the effectiveness and posed a threat to public health. The purpose of this study was to summarize the complete plasmids bearing mcr and explore plasmid characteristics that might affect the spread of colistin resistance. A total of 103 complete plasmid sequences carrying mcr which were released in GenBank from 2015 to 2018 were analyzed. The results showed that the main plasmids were from China and Escherichia coli was the main host. IncI2 and IncX4 were the most important replicon types, and IncHI2 was the dominant type of plasmids with multiple resistance genes. Meanwhile, 95.14% of the plasmids contained antibacterial biocide or heavy metal resistance genes. Except for ISApl1, the frequency of IS26 was the highest. 18.45% of them had conjugated transfer elements, including oriT, relaxase, T4CP and T4SS. This study emphasized the co-selection of antibiotics and heavy metals, which provided landscape for better control of colistin resistance.

Key words: colistin, mcr-1, complete plasmid sequence, multi-drug resistance

CLC Number:

S852.61

TANG Biao, CHANG Jiang, HU Ji, QIAN Mingrong, XIA Xiaodong, YANG Hua. Structures and characteristics of 103 plasmids carrying mcr gene[J]. Acta Agriculturae Zhejiangensis, 2021, 33(1): 43-51.

Figures/Tables 8

Fig.1 Geographical origin of 103 plasmids carrying mcr gene

Fig.2 Hosts of 103 plasmids carrying mcr gene

Fig.3 Distribution of replication type of 103 plasmids carrying mcr gene

Fig.4 Distribution of antimicrobial resistance genes of 103 plasmids carrying mcr gene

Fig.5 Drug resistance phenotypes mediated by 103 plasmids carrying mcr gene

Fig.6 Numbers of antimicrobial resistance genes in 103 plasmids carrying mcr gene

Fig.7 Numbers of antibacterial biocide and heavy metal-resistance genes in 103 plasmids carrying mcr gene

Fig.8 Numbers of mobile elements in 103 plasmids carrying mcr gene

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Structures and characteristics of 103 plasmids carrying mcr gene

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