Growth-promoting characteristics of Aspergillus niger TL-F2 and its effect on seed germination and cadmium content in seedlings of ryegrass under cadmium stress

doi:10.3969/j.issn.1004-1524.2021.02.16

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (2): 326-334.DOI: 10.3969/j.issn.1004-1524.2021.02.16

• Plant Protection • Previous Articles Next Articles

Growth-promoting characteristics of Aspergillus niger TL-F2 and its effect on seed germination and cadmium content in seedlings of ryegrass under cadmium stress

LIU Ru(), DONG Changru, ZHANG Yiwen, QU Minghui, ZHANG Wei, SA Haiyang, CHEN Haiyan, YE Wenling, FAN Ting^*()

Anhui Province Key Lab of Farmland Ecological Conservation and Pollution Prevention, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China

Received:2020-08-17 Online:2021-02-25 Published:2021-02-25
Contact: FAN Ting

Abstract

Abstract:

The growth-promoting characteristics of Aspergillus niger TL-F2, and its effects on seed germination, seedling growth and Cd content of ryegrass under Cd stress were studied. The results showed that the siderophore production of A. niger TL-F2 was significantly (P<0.05) decreased by 48.18%, and there was no significant change of indoleacetic acid (IAA) production and phosphate solubilization under low Cd (5 mg·L^-1) stress. However, under medium (20 mg·L^-1) and high (50 mg·L^-1) Cd stress, the IAA production, phosphate solubilization and siderophore production of A. niger TL-F2 was significantly (P<0.05) decreased by 55.76% and 65.69%, 50.07% and 78.19%, 69.71% and 80.08%, respectively. Inoculation of 1×10⁸ mL^-1 A. niger TL-F2 could benefit the seed germination and seedling growth of ryegrass, and the Cd content in shoots was significantly (P<0.05) increased by 17.95% than that without inoculation under high Cd stress. However, inoculation of 1×10⁶ mL^-1 A. niger TL-F2 showed no significant effect on seed germination and seedling growth of ryegrass. The results suggested that inoculation of proper amount of A. niger TL-F2 could promote seed germination and seedling growth of ryegrass, and enhance the accumulation of Cd in the shoots of ryegrass seedlings. Therefore, inoculation of A. niger TL-F2 had the potential as a new means to enhance the remediation of Cd polluted soil and water by using ryegrass.

Key words: ryegrass, cadmium, Aspergillus niger, seed germination, growth-promoting characteristics

CLC Number:

X172

LIU Ru, DONG Changru, ZHANG Yiwen, QU Minghui, ZHANG Wei, SA Haiyang, CHEN Haiyan, YE Wenling, FAN Ting. Growth-promoting characteristics of Aspergillus niger TL-F2 and its effect on seed germination and cadmium content in seedlings of ryegrass under cadmium stress[J]. Acta Agriculturae Zhejiangensis, 2021, 33(2): 326-334.

Figures/Tables 6

References 45

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Cd/ (mg·L^-1)	菌浓度 Stain content/mL^-1	GV/%	GR/%	GI	VI	TI	SI
0	0	72±2 ab	90±1 a	45.03±0.93 a	736.22±15.18 b	1.00±0.01 ab	0 de
0	1×10⁶	66±4 bcd	88±3 a	42.80±2.04 ab	692.11±32.93 c	1.00±0.15 ab	-0.01±0.15 de
0	1×10⁸	78±3 a	90±3 a	46.22±1.43 a	839.05±26.01 a	1.13±0.16 a	-0.13±0.16 e
5	0	70±2 abc	90±2 a	44.39±0.95 ab	592.68±12.62 d	0.82±0.06 bc	0.18±0.03 cd
5	1×10⁶	66±4 bcd	88±2 a	42.69±1.08 ab	604.43±15.34 d	0.87±0.06 b	0.13±0.06 d
5	1×10⁸	74±3 ab	90±3 a	45.11±1.82 a	828.25±33.27 a	1.14±0.18 a	-0.14±0.18 e
20	0	60±3 cd	84±6 a	40.45±3.15 ab	251.19±19.57 f	0.38±0.04 d	0.62±0.04 b
20	1×10⁶	56±9 d	84±5 a	39.13±2.88 b	191.362±14.06 g	0.30±0.02 d	0.70±0.02 b
20	1×10⁸	66±5 bcd	88±2 a	42.77±1.69 ab	425.98±16.81 e	0.62±0.20 c	0.38±0.20 b
50	0	32±6 f	64±7 b	26.37±3.47 c	0 h	0 e	1.00 a
50	1×10⁶	28±6 f	64±6 b	26.45±3.02 c	0 h	0 e	1.00 a
50	1×10⁸	44±7 e	64±8 b	30.19±4.12 c	0 h	0 e	1.00 a

Cd/ (mg·L^-1)	菌浓度 Stain content/mL^-1	GV/%	GR/%	GI	VI	TI	SI
0	0	72±2 ab	90±1 a	45.03±0.93 a	736.22±15.18 b	1.00±0.01 ab	0 de
0	1×10⁶	66±4 bcd	88±3 a	42.80±2.04 ab	692.11±32.93 c	1.00±0.15 ab	-0.01±0.15 de
0	1×10⁸	78±3 a	90±3 a	46.22±1.43 a	839.05±26.01 a	1.13±0.16 a	-0.13±0.16 e
5	0	70±2 abc	90±2 a	44.39±0.95 ab	592.68±12.62 d	0.82±0.06 bc	0.18±0.03 cd
5	1×10⁶	66±4 bcd	88±2 a	42.69±1.08 ab	604.43±15.34 d	0.87±0.06 b	0.13±0.06 d
5	1×10⁸	74±3 ab	90±3 a	45.11±1.82 a	828.25±33.27 a	1.14±0.18 a	-0.14±0.18 e
20	0	60±3 cd	84±6 a	40.45±3.15 ab	251.19±19.57 f	0.38±0.04 d	0.62±0.04 b
20	1×10⁶	56±9 d	84±5 a	39.13±2.88 b	191.362±14.06 g	0.30±0.02 d	0.70±0.02 b
20	1×10⁸	66±5 bcd	88±2 a	42.77±1.69 ab	425.98±16.81 e	0.62±0.20 c	0.38±0.20 b
50	0	32±6 f	64±7 b	26.37±3.47 c	0 h	0 e	1.00 a
50	1×10⁶	28±6 f	64±6 b	26.45±3.02 c	0 h	0 e	1.00 a
50	1×10⁸	44±7 e	64±8 b	30.19±4.12 c	0 h	0 e	1.00 a

Cd/ (mg·L^-1)	菌浓度 Stain content/mL^-1	根长 Root length/cm	芽长 Shoot length/cm	根干重 Root dry weight/g	芽干重 Shoot dry weight/g
0	0	5.45±0.75 ab	13.90±0.34 a	0.011±0.003 a	0.044±0.011 a
0	1×10⁶	5.39±0.69 ab	13.82±0.47 a	0.013±0.001 a	0.044±0.007 a
0	1×10⁸	6.05±0.42 a	14.50±0.72 a	0.014±0.001 a	0.044±0.007 a
5	0	4.45±0.47 b	12.16±0.93 b	0.013±0.001 a	0.042±0.002 a
5	1×10⁶	4.72±0.29 b	10.97±0.80 c	0.013±0.001 a	0.045±0.003 a
5	1×10⁸	6.12±0.77 a	12.36±0.81 b	0.015±0.003 a	0.042±0.008 a
20	0	2.07±0.51 d	9.37±0.85 d	0.011±0.002 a	0.037±0.004 a
20	1×10⁶	1.63±0.26 d	8.81±0.21 e	0.015±0.005 a	0.038±0.007 a
20	1×10⁸	3.32±1.08 c	9.95±0.92 cd	0.014±0.001 a	0.044±0.007 a
50	0	—	7.27±0.67 e	—	0.036±0.007 a
50	1×10⁶	—	5.83±0.07 f	—	0.034±0.005 a
50	1×10⁸	—	7.52±0.72 e	—	0.036±0.007 a

Cd/ (mg·L^-1)	菌浓度 Stain content/mL^-1	根长 Root length/cm	芽长 Shoot length/cm	根干重 Root dry weight/g	芽干重 Shoot dry weight/g
0	0	5.45±0.75 ab	13.90±0.34 a	0.011±0.003 a	0.044±0.011 a
0	1×10⁶	5.39±0.69 ab	13.82±0.47 a	0.013±0.001 a	0.044±0.007 a
0	1×10⁸	6.05±0.42 a	14.50±0.72 a	0.014±0.001 a	0.044±0.007 a
5	0	4.45±0.47 b	12.16±0.93 b	0.013±0.001 a	0.042±0.002 a
5	1×10⁶	4.72±0.29 b	10.97±0.80 c	0.013±0.001 a	0.045±0.003 a
5	1×10⁸	6.12±0.77 a	12.36±0.81 b	0.015±0.003 a	0.042±0.008 a
20	0	2.07±0.51 d	9.37±0.85 d	0.011±0.002 a	0.037±0.004 a
20	1×10⁶	1.63±0.26 d	8.81±0.21 e	0.015±0.005 a	0.038±0.007 a
20	1×10⁸	3.32±1.08 c	9.95±0.92 cd	0.014±0.001 a	0.044±0.007 a
50	0	—	7.27±0.67 e	—	0.036±0.007 a
50	1×10⁶	—	5.83±0.07 f	—	0.034±0.005 a
50	1×10⁸	—	7.52±0.72 e	—	0.036±0.007 a

Growth-promoting characteristics of Aspergillus niger TL-F2 and its effect on seed germination and cadmium content in seedlings of ryegrass under cadmium stress

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