Genetic analysis of stripe rust resistance genes in 2 spring wheat germplasm resources at adult stage

doi:10.3969/j.issn.1004-1524.2021.04.04

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (4): 595-601.DOI: 10.3969/j.issn.1004-1524.2021.04.04

• Crop Science • Previous Articles Next Articles

Genetic analysis of stripe rust resistance genes in 2 spring wheat germplasm resources at adult stage

ZHAO Ke¹(), LI Qiurong¹, HOU Lu¹^,^*(), BAI Yaobo², JIANG Liling¹, WEI Youhai¹, GUO Qingyun¹

1. Key Laboratory of Agricultural Integrated Pest Management, Qinghai University, Qinghai Academy of Agricultural and Forestry Sciences, Xining 810016, China
2. Xuzhou Key Laboratory of Modern Agrobiotechnology Engineering, Xuzhou Vocational College of Bioengineering, Xuzhou 221006, China

Received:2020-12-23 Online:2021-04-25 Published:2021-04-25
Contact: HOU Lu

Abstract

Abstract:

Analysis of the genetic rules of the stripe rust resistance genes of spring wheat at adult stage can provide theoretical basis for the utilization of stripe rust resistance genes. In this study, stripe rust genes genetic analysis was carried out in the field for 2 years by using F_2:3 generation segregating populations made by susceptible variety Taichung29 crossed with MY004730, and ZM018243 crossed with MY004730. Results showed that, disease severity and infection type of the 2 F_2:3 populations showed discrete distribution and didn’t conform to normal distribution. It is speculated that resources the stripe rust resistance of MY004730 and ZM018243 at adult stage has qualitative character. Resistance and susceptible segregation ratio of F_2:3 population constructed by crossing of Taichung29 and MY004730 was 3R:1S, indicating that resistance of MY004730 to stripe rust at adult plant stage was controlled by 1 dominant gene; while F_2:3 population by ZM018243 crossing with MY004730, the single plants/lines resistance and susceptible segregation ratio was 9R:7S, that is conformed to 2 pairs of dominant genes work together. Therefore, it is that ZM018243 may contain 1 pair of dominant stripe rust resistance gene adult plant stage. According to the genetic analysis results, fuether research can be focused on molecular mapping of resistance genes to find markers closely linked to resistance genes, and serve for molecular marker-assisted selection breeding. At the same time, resistant gene aggregation materials can be screened from the resistance-resistance crossing population, hence provide excellent resistance materials.

Key words: spring wheat, stripe rust resistance gene, germplasm resources, genetic analysis

CLC Number:

ZHAO Ke, LI Qiurong, HOU Lu, BAI Yaobo, JIANG Liling, WEI Youhai, GUO Qingyun. Genetic analysis of stripe rust resistance genes in 2 spring wheat germplasm resources at adult stage[J]. Acta Agriculturae Zhejiangensis, 2021, 33(4): 595-601.

Figures/Tables 3

Fig.1 Normal probability of Taichung 29/MY004730 (A) and ZM018243/MY004730 (B) F2 population response type and disease severity

Fig.2 Frequency distribution of infection type and disease severity of F2∶3, populations from Taichung 29/MY004730 and ZM018243/MY004730tested in Xining P1,Taichung29;P2,MY004730;P3,ZM018243。

Table 1 Genetic analysis of disease resistance in Taichung 29/MY004730 and ZM018243/MY004730 F2:3 populations

杂交组合 Cross combination	年份 Year	后代 Generation	抗病植株数 No. of resistant plants	感病植株数 No. of susceptible plants	期望比 Expected ratio(R:S)	χ²	P
Taichung29/MY004730	2017	F₂	130	45	3∶1	0.02	0.83
	2019	F₃	131	44	3∶1	<0.01	0.97
ZM018243/MY004730	2017	F₂	89	71	9∶7	<0.01	0.87
	2019	F₃	79	68	9∶7	0.28	0.54

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Genetic analysis of stripe rust resistance genes in 2 spring wheat germplasm resources at adult stage

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