Identification of soybean LAZ1 gene family and functional analysis of GmLAZ1-9

doi:10.3969/j.issn.1004-1524.2021.04.03

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (4): 586-594.DOI: 10.3969/j.issn.1004-1524.2021.04.03

• Crop Science • Previous Articles Next Articles

Identification of soybean LAZ1 gene family and functional analysis of GmLAZ1-9

YANG Xinxia^a(), ZHANG Bin^b^,^*()

a. Department of Logistics
b. College of Chemistry and Bioengineering, Hunan University of Science and Engineering, Yongzhou 425199, China

Received:2020-10-11 Online:2021-04-25 Published:2021-04-25
Contact: ZHANG Bin

Abstract

Abstract:

In the present study, the BLAST search was performed in the soybean genome database and LAZ1 gene family members were obtained. Bioinformatics analysis and expression pattern detection were performed, and transgenic Arabidopsis was used to verify gene function. It was shown that a total of 17 soybean LAZ1 genes were identified, namely GmLAZ1-1 to GmLAZ1-17. All of the GmLAZ1 genes were unevenly distributed on 12 chromosomes. The LAZ1 family members in soybean were phylogenetically divided into three evolutionary clades, and the members in the same clades shared similar gene and protein structures. The analysis of cis-regulatory elements indicated that multiple abiotic stress-related cis-acting elements presented in the promoters of soybean LAZ1 genes, such as ABRE, ARE, LTR, G-box, etc. Semi-quantitative PCR results showed that the expression of GmLAZ1-5, GmLAZ1-9, GmLAZ1-11, and GmLAZ1-13 was up-regulated after salt treatment. Moreover, GmLAZ1-9 enhanced salinity tolerance in transgenic Arabidopsis, which provided basis for further exploration of the functions and molecular mechanisms of other LAZ1 genes.

Key words: soybean, LAZ1 family, salt stress, gene expression

CLC Number:

S565.1
Q78

YANG Xinxia, ZHANG Bin. Identification of soybean LAZ1 gene family and functional analysis of GmLAZ1-9[J]. Acta Agriculturae Zhejiangensis, 2021, 33(4): 586-594.

Figures/Tables 5

Table 1 Physical and chemical parameters of genes in soybean LAZ1 family

基因名 Name	基因编号 Gene ID	基因长度 Gene length/bp	氨基酸长度 Peptide residues/aa	相对分子质量 Molecular weight/ku	pI	不稳定系数 Instability index
GmLAZ1-1	Glyma.03G226700	5 087	421	47.17	6.78	44.35
GmLAZ1-2	Glyma.04G001400	5 252	486	54.56	8.75	44.04
GmLAZ1-3	Glyma.04G240300	5 229	288	32.65	5.85	49.15
GmLAZ1-4	Glyma.06G000800	5 037	492	55.03	8.86	41.80
GmLAZ1-5	Glyma.06G123100	5 818	485	55.25	6.77	47.60
GmLAZ1-6	Glyma.06G253700	6 131	304	35.01	8.79	26.55
GmLAZ1-7	Glyma.09G071000	7 053	440	49.44	4.35	42.21
GmLAZ1-8	Glyma.10G141000	7 265	418	46.92	8.23	41.65
GmLAZ1-9	Glyma.12G147700	9 833	287	33.18	8.87	31.34
GmLAZ1-10	Glyma.13G038900	7 402	484	54.98	5.56	46.63
GmLAZ1-11	Glyma.13G245500	2 971	296	34.07	8.80	43.68
GmLAZ1-12	Glyma.14G122700	8 650	484	55.12	5.99	44.47
GmLAZ1-13	Glyma.15G068200	2 547	296	34.13	8.59	45.68
GmLAZ1-14	Glyma.15G178900	4 890	397	44.60	8.84	32.32
GmLAZ1-15	Glyma.17G156700	4 054	384	44.25	9.56	37.51
GmLAZ1-16	Glyma.19G223700	4 841	419	46.68	6.31	41.23
GmLAZ1-17	Glyma.20G089700	3 907	418	46.77	8.41	40.32

Fig.1 Bioinformatics analysis of soybean LAZ1 family members A, Chromosomal location of soybean LAZ1 family genes; B, Phylogenetic tree of members of the LAZ1 family of soybeans and other species (Arabidopsis, rice, and maize); C, Analysis of gene structure and amino acid conservative motifs of soybean LAZ1 family members.

Fig.2 Expression patterns of soybean LAZ1 genes in different tissues The color scale on the right represents the level of gene expression. From gray to red, the value of expression increases from low to high.

Fig.3 Cis-acting element in soybean LAZ1 gene promoters and expression patterns of LAZ1 genes under salt treatment A, Abiotic stress-related cis-acting elements in the promoters of soybean LAZ1 genes; B, Expression of LAZ1 genes at 0, 3, 6, 12 h after treatment with 150 mmol·L-1 NaCl based on semi-quantitative PCR test.

Fig.4 Overexpression of soybean GmLAZ1-9 gene enhanced salt tolerance of transgenic Arabidopsis Phenotype (A) and germination rate (B) of the wild-type Arabidopsis WT and the transgenic material OEGmLAZ1-9 seeds for 7 days on 1/2MS medium and 1/2MS+200 mmol·L-1 NaCl medium; Phenotype (C), relative water content (D), and content of MDA (E) and proline (F) of 4-week-old Arabidopsis(WT and OEGmLAZ1-9) grown in normal condition and 150 mmol·L-1 NaCl solution for 7 days. Bars in D-F marked with “*” indicated significant difference between test materials at P<0.05 under the same condition.

References 18

[1]	LIU B L, YU H Q, WEN Q, et al. Genome-wide analysis of LAZ1 gene family from maize[J]. Journal of Plant Growth Regulation, 2020,39(2):656-668.
[2]	MALINOVSKY F G, BRODERSEN P, FIIL B K, et al. Lazarus1, a DUF300 protein, contributes to programmed cell death associated with Arabidopsis acd11 and the hypersensitive response[J]. PLoS One, 2010,5(9):e12586. URL PMID
[3]	DAWSON P A, HUBBERT M L, RAO A. Getting the mOST from OST: role of organic solute transporter, OSTα-OSTβ, in bile acid and steroid metabolism[J]. Biochimica et Biophysica Acta: Molecular and Cell Biology of Lipids, 2010,1801(9):994-1004. URL PMID
[4]	OLIVIER-MASON A, WOJTYNIAK M, BOWIE R V, et al. Transmembrane protein OSTA-1 shapes sensory Cilia morphology via regulation of intracellular membrane trafficking in C. elegans[J]. Development (Cambridge, England), 2013,140(7):1560-1572. URL PMID
[5]	LIU Q S, VAIN T, VIOTTI C, et al. Vacuole integrity maintained by DUF300 proteins is required for brassinosteroid signaling regulation[J]. Molecular Plant, 2018,11(4):553-567. DOI URL PMID
[6]	明川, 拓昊苑, 陶怡, 等. 玉米蔗糖非酵解型蛋白激酶2底物蛋白的筛选与鉴定[J]. 西北农林科技大学学报(自然科学版), 2018,46(10):57-67.
	MING C, TUO H Y, TAO Y, et al. Screening and identification of substrate proteins of sucrose non-fermenting protein kinase 2 in maize[J]. Journal of Northwest A & F University (Natural Science Edition), 2018,46(10):57-67.(in Chinese with English abstract)
[7]	WANG Y G, FU F L, YU H Q, et al. Interaction network of core ABA signaling components in maize[J]. Plant Molecular Biology, 2018,96(3):245-263. DOI URL PMID
[8]	ZHU J K. Abiotic stress signaling and responses in plants[J]. Cell, 2016,167(2):313-324. URL PMID
[9]	GONG Z Z, XIONG L M, SHI H Z, et al. Plant abiotic stress response and nutrient use efficiency[J]. Science China Life Sciences, 2020,63(5):635-674. URL PMID
[10]	ZHAO C Z, ZHANG H, SONG C P, et al. Mechanisms of plant responses and adaptation to soil salinity[J]. The Innovation, 2020,1(1):100017.
[11]	王丽, 王万兴, 索海翠, 等. 马铃薯多酚氧化酶基因家族生物信息学及表达分析[J]. 湖南农业大学学报(自然科学版), 2019,45(6):601-610.
	WANG L, WANG W X, SUO H C, et al. Bioinformatics and expression analysis of polyphenol oxidase gene family in potato[J]. Journal of Hunan Agricultural University (Natural Sciences), 2019,45(6):601-610.(in Chinese with English abstract)
[12]	ZHANG B, LIU J, YANG Z E, et al. Genome-wide analysis of GRAS transcription factor gene family in Gossypium hirsutum L[J]. BMC Genomics, 2018,19(1):1-12. URL PMID
[13]	JIA H H, WANG C, WANG F, et al. GhWRKY68 reduces resistance to salt and drought in transgenic Nicotiana benthamiana[J]. PLoS One, 2015,10(3):e0120646. DOI URL PMID
[14]	MANZI M, LADO J, RODRIGO M J, et al. ABA accumulation in water-stressed citrus roots does not rely on carotenoid content in this organ[J]. Plant Science, 2016,252:151-161.
[15]	YOUSFI F E, MAKHLOUFI E, MARANDE W, et al. Comparative analysis of WRKY genes potentially involved in salt stress responses in Triticum turgidum L. ssp. durum[J]. Frontiers in Plant Science, 2017,7:2034. URL PMID
[16]	朱作峰, 孙传清, 付永彩, 等. 水稻中一个新的MYC基因的克隆及其分析[J]. 遗传学报, 2005,32(4):393-398.
	ZHU Z F, SUN C Q, FU Y C, et al. Isolation and analysis of a novel MYC gene from rice[J]. Acta Genetica Sinica, 2005,32(4):393-398.(in Chinese with English abstract)
[17]	LI Z Y, XU Z S, HE G Y, et al. A mutation in Arabidopsis BSK₅ encoding a brassinosteroid-signaling kinase protein affects responses to salinity and abscisic acid[J]. Biochemical and Biophysical Research Communications, 2012,426(4):522-527. URL PMID
[18]	MAESTRINI P, CAVALLINI A, RIZZO M, et al. Isolation and expression analysis of low temperature-induced genes in white poplar (Populus alba)[J]. Journal of Plant Physiology, 2009,166(14):1544-1556. URL PMID

Identification of soybean LAZ1 gene family and functional analysis of GmLAZ1-9

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 5

References 18

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHANG Jun, ZHANG Bo, HU Bibo, LIU Jingliang, ZHANG Xiaoyu, LI Chunyang, XIONG Shengting, GUO Binbin, WANG Xiucun, MA Chao. Identification and expression analysis of members of the SWEET and SUT families in wheat (Triticum aestivum L.) [J]. Acta Agriculturae Zhejiangensis, 2025, 37(9): 1825-1839.
[2]	HU Yingjie, DU Chenqi, WANG Liufan, SHOU Jianxin, WANG Chao, XU Mei, YAN Xu. Research progress of vesicle trafficking in plant response to salt stress [J]. Acta Agriculturae Zhejiangensis, 2025, 37(9): 2003-2011.
[3]	GUAN Xiusheng, LIU Tieshan, WANG Juan, ZHANG Maolin, LIU Chunxiao, DONG Rui, GUAN Haiying, LIU Qiang, XU Yang, HE Chunmei. Bioinformatics analysis and cloning of NF-YA family genes in maize(Zea mays) [J]. Acta Agriculturae Zhejiangensis, 2025, 37(8): 1605-1614.
[4]	HE Guoxin, LI Sujuan, WANG Jian, TAO Xiaoyuan, YE Zihong, CHEN Guang, XU Shengchun. Screening and identification of soybean germplasm for low nitrogen tolerance during seedling stage [J]. Acta Agriculturae Zhejiangensis, 2025, 37(5): 965-976.
[5]	XU Zhuwei, LEI Jun, SHAO Xiaowei, CHEN Runxing, JIANG Huan, WANG Shougen, YU Wenhui. Evaluation of Quzhou fresh soybean oligosaccharide germplasm resources based on analytic hierarchy process and fuzzy comprehensive evaluation method [J]. Acta Agriculturae Zhejiangensis, 2025, 37(4): 754-766.
[6]	TANG Aoran, JIN Xiu, WANG Tan, RAO Yuan, LI Jiajia, ZHANG Wu. Physiological plant height measurement method based on the reconstruction of the main stem skeleton for curved soybean plants [J]. Acta Agriculturae Zhejiangensis, 2025, 37(2): 466-479.
[7]	LIAO Xiaolong, WANG Xingsheng, CHEN Yong, LI Bin, HONG Sidan, MEI Lina, GUO Ying. Identification of the HKT gene family members in Populus species and analysis of their expression patterns under salt stress [J]. Acta Agriculturae Zhejiangensis, 2025, 37(10): 2104-2115.
[8]	OU Jinwen, ZHANG Guwen, FENG Zhijuan, WANG Bin, BU Yuanpeng, XU Yu, RU Lei, LIU Na, GONG Yaming. Identification of soybean trehalose-6-phosphate phosphatase gene GmTPP and its expression analysis in growth and abiotic stress response [J]. Acta Agriculturae Zhejiangensis, 2024, 36(9): 2031-2041.
[9]	HE Changxi, ZHENG Jianbo, MA Jianbo, JIA Yongyi, LIU Shili, JIANG Wenping, CHI Meili, CHENG Shun, LI Fei. Cloning and expression analysis of Runx2b in Culter alburnus [J]. Acta Agriculturae Zhejiangensis, 2024, 36(5): 1024-1031.
[10]	LI Yaping, JIN Fulai, HUANG Zonggui, ZHANG Tao, DUAN Xiaojing, JIANG Wu, TAO Zhengming, CHEN Jiadong. Identification and expression pattern analysis of glycoside hydrolase GH3 gene family in Dendrobium officinale [J]. Acta Agriculturae Zhejiangensis, 2024, 36(4): 790-799.
[11]	PENG Jiacheng, WU Yue, XU Jiehao, XIA Meiwen, QI Tianpeng, XU Haisheng. Cloning of paxillin gene from Macrobrachium nipponense and effect of cadmium stress on its expression [J]. Acta Agriculturae Zhejiangensis, 2024, 36(2): 247-253.
[12]	LIU Xiaolin, SUN Tingting, YANG Jie, HE Hengbin. Cloning and expression analysis of FLS gene of flavonol synthetase in Lilium auratum and L.speciosum var. gloriosoides [J]. Acta Agriculturae Zhejiangensis, 2024, 36(2): 344-357.
[13]	GAO Jing, LU Linghong, GU Xianbin, FAN Fei, SONG Genhua, ZHANG Huiqin. Cloning of AcWRKY94 gene from kiwifruit and its functional analysis under salt stress [J]. Acta Agriculturae Zhejiangensis, 2024, 36(11): 2501-2509.
[14]	ZHAO Lingji, LIAO Xiangjiao, LIU Dechun, HU Wei, KUANG Liuqing, SONG Jie, YI Mingliang, LIU Yong, YANG Li. Changes of organic acid content in Taoxi pomelo fruits during the storage period and citric acid related gene expression analysis [J]. Acta Agriculturae Zhejiangensis, 2024, 36(11): 2510-2520.
[15]	TANG Yuehui, CHEN Shuying, HE Wenqiong, WANG Hanjin, BAO Xinxin, JIA Sainan, WANG Yaoyao, CHEN Yuyang, YANG Tongwen. Cloning and functional analysis of JcERF22 gene from Jatropha curcas [J]. Acta Agriculturae Zhejiangensis, 2024, 36(10): 2219-2228.