Identification of soybean trehalose-6-phosphate phosphatase gene GmTPP and its expression analysis in growth and abiotic stress response

doi:10.3969/j.issn.1004-1524.20231003

Acta Agriculturae Zhejiangensis ›› 2024, Vol. 36 ›› Issue (9): 2031-2041.DOI: 10.3969/j.issn.1004-1524.20231003

• Horticultural Science • Previous Articles Next Articles

Identification of soybean trehalose-6-phosphate phosphatase gene GmTPP and its expression analysis in growth and abiotic stress response

OU Jinwen¹^,²(), ZHANG Guwen², FENG Zhijuan², WANG Bin², BU Yuanpeng², XU Yu², RU Lei¹, LIU Na¹^,²^,^*(), GONG Yaming²

1. College of Horticultural Science, Zhejiang A&F University, Hangzhou 311300, China
2. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2023-08-22 Online:2024-09-25 Published:2024-09-30

Abstract

Abstract:

Trehalose plays an important role in plant metabolism, growth, development and stress resistance. The gene for trehalose-6-phosphate phosphatase (TPP) is crucial for the biosynthesis of trehalose. Soybeans, an important legume crop, are rich in protein and oil in their seeds, and there have been few reports on their TPP gene family. To analyze the structure and function of TPP genes in soybeans, 15 GmTPP genes were identified using bioinformatics methods across the entire soybean genome. Phylogenetic analysis indicated that these 15 GmTPP genes could be categorized into three subfamilies, with each GmTPP containing 9-11 introns, and the number of introns being the same within the same subfamily. Analysis of cis-acting elements suggested that GmTPP genes were involved in plant hormone and environmental stress responses. Furthermore, we utilized transcriptome data to study the expression patterns of these GmTPP genes in different tissues and their expression characteristics under various abiotic stresses. The results showed that GmTPP genes had specific expression patterns in all soybean tissues and organs and exhibited different expressions under salt and drought stress treatments. This study not only lays the foundation for revealing the role of the GmTPP gene family in the regulation of trehalose in soybeans but also provides some reference value for improving soybean stress resistance using the TPP gene family.

Key words: trehalose-6-phosphate phosphatase gene GmTPP, soybean, abiotic stress, expression analysis

CLC Number:

S565.1

OU Jinwen, ZHANG Guwen, FENG Zhijuan, WANG Bin, BU Yuanpeng, XU Yu, RU Lei, LIU Na, GONG Yaming. Identification of soybean trehalose-6-phosphate phosphatase gene GmTPP and its expression analysis in growth and abiotic stress response[J]. Acta Agriculturae Zhejiangensis, 2024, 36(9): 2031-2041.

Figures/Tables 9

References 41

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基因 Gene	基因序列号 Gene ID number	染色体 Chromosome	内含子数目 Number of introns	氨基酸数 Number of amino acids	分子量 Molecular mass/ku	等电点 Isoelectric point(pI)	亚细胞定位 Subcellular localization
GmTPP1	Glyma.04G103600	Gm04	9	371	41.438 67	9.35	叶绿体Chloroplast
GmTPP2	Glyma.04G119700	Gm04	11	340	37.565 88	5.52	叶绿体Chloroplast
GmTPP3	Glyma.04G237900	Gm04	9	367	41.772 01	8.86	叶绿体Chloroplast
GmTPP4	Glyma.05G179800	Gm05	10	277	32.010 92	8.89	过氧化物酶体Peroxisomal
GmTPP5	Glyma.06G104800	Gm06	9	370	41.444 76	9.46	叶绿体Chloroplast
GmTPP6	Glyma.06G126300	Gm06	10	367	42.046 28	9.09	叶绿体Chloroplast
GmTPP7	Glyma.08G137500	Gm08	10	290	33.448 61	8.83	过氧化物酶体Peroxisomal
GmTPP8	Glyma.09G231400	Gm09	11	389	43.419 69	8.54	细胞质Cytoplasmic
GmTPP9	Glyma.11G239300	Gm11	10	363	41.038 08	9.31	叶绿体Chloroplast
GmTPP10	Glyma.12G005200	Gm12	11	389	43.276 45	7.66	叶绿体Chloroplast
GmTPP11	Glyma.13G088300	Gm13	9	372	41.824 2	9.29	叶绿体Chloroplast
GmTPP12	Glyma.14G171700	Gm14	9	379	42.540 85	9.47	叶绿体Chloroplast
GmTPP13	Glyma.16G025600	Gm16	9	203	23.115 63	6.53	细胞质Cytoplasmic
GmTPP14	Glyma.17G138700	Gm17	11	362	41.039 38	5.79	细胞核Nuclear
GmTPP15	Glyma.18G018100	Gm18	11	365	41.200 30	9.31	叶绿体Chloroplast

基因 Gene	基因序列号 Gene ID number	染色体 Chromosome	内含子数目 Number of introns	氨基酸数 Number of amino acids	分子量 Molecular mass/ku	等电点 Isoelectric point(pI)	亚细胞定位 Subcellular localization
GmTPP1	Glyma.04G103600	Gm04	9	371	41.438 67	9.35	叶绿体Chloroplast
GmTPP2	Glyma.04G119700	Gm04	11	340	37.565 88	5.52	叶绿体Chloroplast
GmTPP3	Glyma.04G237900	Gm04	9	367	41.772 01	8.86	叶绿体Chloroplast
GmTPP4	Glyma.05G179800	Gm05	10	277	32.010 92	8.89	过氧化物酶体Peroxisomal
GmTPP5	Glyma.06G104800	Gm06	9	370	41.444 76	9.46	叶绿体Chloroplast
GmTPP6	Glyma.06G126300	Gm06	10	367	42.046 28	9.09	叶绿体Chloroplast
GmTPP7	Glyma.08G137500	Gm08	10	290	33.448 61	8.83	过氧化物酶体Peroxisomal
GmTPP8	Glyma.09G231400	Gm09	11	389	43.419 69	8.54	细胞质Cytoplasmic
GmTPP9	Glyma.11G239300	Gm11	10	363	41.038 08	9.31	叶绿体Chloroplast
GmTPP10	Glyma.12G005200	Gm12	11	389	43.276 45	7.66	叶绿体Chloroplast
GmTPP11	Glyma.13G088300	Gm13	9	372	41.824 2	9.29	叶绿体Chloroplast
GmTPP12	Glyma.14G171700	Gm14	9	379	42.540 85	9.47	叶绿体Chloroplast
GmTPP13	Glyma.16G025600	Gm16	9	203	23.115 63	6.53	细胞质Cytoplasmic
GmTPP14	Glyma.17G138700	Gm17	11	362	41.039 38	5.79	细胞核Nuclear
GmTPP15	Glyma.18G018100	Gm18	11	365	41.200 30	9.31	叶绿体Chloroplast

Identification of soybean trehalose-6-phosphate phosphatase gene GmTPP and its expression analysis in growth and abiotic stress response

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