Identification and expression analysis of sucrose transporter SUT family in watermelon in fruit development and stress responses

doi:10.3969/j.issn.1004-1524.20230188

Acta Agriculturae Zhejiangensis ›› 2024, Vol. 36 ›› Issue (1): 94-102.DOI: 10.3969/j.issn.1004-1524.20230188

• Horticultural Science • Previous Articles Next Articles

Identification and expression analysis of sucrose transporter SUT family in watermelon in fruit development and stress responses

SHOU Weisong(), WANG Duo, SHEN Jia, XU Xinyang, ZHANG Yuejian, HE Yanjun()

Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2023-02-17 Online:2024-01-25 Published:2024-02-18

Abstract

Abstract:

Sucrose transporter (SUTs) gene, as the main active transport vector of sucrose, plays a key role in the transport of allotment assimilates from source tissue to sink tissue. Although the characteristics and biological functions of SUTs have been well studied in higher plants, the SUT gene family has not been identified in watermelon. In this study, four ClSUT genes were identified in watermelon genome, and their gene and protein structure, conserved motifs, subcellular localization, chromosome distribution, evolutionary relationships and promoter cis-elements were comprehensively analyzed. Gene structure analysis showed that ClSUT3 and ClSUT4 grouped into subgroup Ⅰ and each only contained one intron. All ClSUT proteins were predicted to be localized in the plasma membrane. All ClSUT proteins contain a conserved MFS domain corresponding to five motifs. Phylogenetic analysis revealed that SUT proteins in Arabidopsis, rice and watermelon were divided into 5 subgroups. ClSUTs in watermelon have more closely relationship with SUT proteins in Arabidopsis and were clustered in subgroup Ⅰ, Ⅱ and Ⅴ. In addition, We also identified several functional cis-elements related to hormone and stress response in the watermelon ClSUT gene promoter. And transcriptome data indicated that watermelon ClSUT genes were variously expressed in response to osmotic and salt stress, which may be involved in watermelon responses to these stresses.

Key words: watermelon, sucrose transporter SUT, fruit development, abiotic stress, expression analysis

CLC Number:

S651

SHOU Weisong, WANG Duo, SHEN Jia, XU Xinyang, ZHANG Yuejian, HE Yanjun. Identification and expression analysis of sucrose transporter SUT family in watermelon in fruit development and stress responses[J]. Acta Agriculturae Zhejiangensis, 2024, 36(1): 94-102.

Figures/Tables 8

Table 1 The basic information of ClSUTs in watermelon

基因名称 Gene name	基因ID Gene ID	染色体位置 Chromosome location	基因方向 Gene direction	氨基酸长度 Protein length/aa	等电点 Isoelectric point	蛋白分子量 Molecular weight/ku	亚细胞定位 Subcellular localization
ClSUT1	Cla97C08G155070.1	Chr08:23133250.. 23138158	-	507	9.36	54.98	质膜 Plasma membrane
ClSUT2	Cla97C09G183410.1	Chr09:36640778.. 36646361	+	604	5.67	64.78	质膜 Plasma membrane
ClSUT3	Cla97C11G215990.1	Chr11:13836471.. 13838050	-	495	8.55	53.15	质膜 Plasma membrane
ClSUT4	Cla97C11G216280.1	Chr11:16625895.. 16627473	+	495	8.84	53.25	质膜 Plasma membrane

Fig.1 The phylogenetic, conserved motif, and domain analysis of ClSUTs in watermelon A, Phylogenetic tree of ClSUTs in watermelon; B, Conserved motifs of ClSUTs in watermelon; C, Conserved domains of ClSUTs in watermelon.

Fig.2 The gene structure of ClSUTs in watermelon

Fig.3 The phylogenetic analyses of SUTs in Arabidopsis, rice and watermelon

Fig.4 The chromosomal localization of ClSUTs in watermelon

Fig.5 The cis-elements in the promoters of ClSUTs in watermelon

Fig.6 Expression heatmap of ClSUTs in watermelon PI296341-FR(A) and 97103 (B) at different fruit developmental periods DAP represents days after pollination.

Fig.7 The expression heatmap of ClSUT in different watermelon in response to osmotic (A) and drought (B) stress CK, Control; OS, Watermelon root tissue under osmotic stress; Y34, Non-drought resistant material; M20, Drought-resistant material.

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Identification and expression analysis of sucrose transporter SUT family in watermelon in fruit development and stress responses

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