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    2024, 36(1):  0-0. 
    Abstract ( 87 )   PDF (3112KB) ( 326 )  
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    Crop Science
    Inhibiting effects of immobilization agents on cadmium, lead and arsenic in rice production
    YANG Xifan, GUO Bin, QIU Gaoyang, LIU Junli, TONG Wenbin, YANG Haijun, ZHU Weidong, MAO Congyan
    2024, 36(1):  1-8.  DOI: 10.3969/j.issn.1004-1524.20230079
    Abstract ( 298 )   HTML ( 612 )   PDF (845KB) ( 564 )  

    In the present study, a field experiment was conducted with different application rates (0, 2 250, 4 500, 6 750 kg·hm-2) of commercial immobilization agents to examine their inhibiting effect on soil available cadmium (Cd), lead (Pb), arsenic (As) contents, soil pH value and Cd, Pb, As contents in rice grains for 2 years (2021—2022). It was shown that application of appropriate rate of immobilization agents significantly (P<0.05) increased soil pH value and decreased soil available Cd, Pb, As contents in soil. Among the 6 immobilization agents, the best effect in 2021 was noted in the treatment of application of 6 750 kg·hm-2 long-acting passivator, as the soil pH value was significanlty increased from 5.00 to 6.98, the soil available Cd, Pb contents were significantly decreased by 82.2%, 98.6%, respectively. Besides, in 2022, application of 4 550 kg·hm-2 long-acting passivator also significantly decreased Cd content in rice grains by 90.8%. In general, the long-acting passivator had the best effect and persistence among all the used immobilization agent, which might be related to its high content of calcium carbonate (CaCO3).

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    Interaction analysis between rice ubiquitin ligase D3 and the disease resistance associated protein VOZ2
    LUO Yingjie, CUI Weijun, WANG Zhonghua, WU Yueyan, LIN Hongyou, ZHOU Jie, YAN Chengqi, WANG Xuming
    2024, 36(1):  9-17.  DOI: 10.3969/j.issn.1004-1524.20221670
    Abstract ( 402 )   HTML ( 424 )   PDF (2736KB) ( 495 )  

    The tiller dwarf gene dwarf-3 (D3) is an important node gene in strigolactone signal transduction in rice. It has been reported that the MAX2 gene in Arabidopsis thaliana is homologous to D3, and MAX2 is involved in the defense response of A. thaliana. In this study, the yeast two-hybrid library was screened with D3 protein as bait, and it was found that there was a potential interaction between rice disease resistance-related protein vascular plant one-zinc finger protein 2 (VOZ2) and D3. The interaction between D3 and VOZ2 was confirmed by yeast two-hybrid validation. Real-time quantitative PCR experiments showed that the expression of VOZ2 gene was significantly induced at the transcriptional level after inoculation with Xanthomonas oryzae pv. oryzae. Subcellular co-localization experiment using rice protoplasts showed that D3 and VOZ2 were co-localized in the nucleus. Bimolecular fluorescence complementation experiments showed that D3 and VOZ2 produced strong fluorescence in the nucleus and cytoplasm of tobacco mesophyll cells, further confirming the interaction between D3 and VOZ2. The study result laid a foundation for further exploring of the function and molecular mechanism of D3 and VOZ2 in rice disease resistance and defense response.

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    Influence of unmanned aerial vehicle observation time on estimation of canopy chlorophyll density of maize
    ZHOU Lili, FENG Haikuan, NIE Chenwei, XU Xiaobin, LIU Yuan, MENG Lin, XUE Beibei, MING Bo, LIANG Qiyun, SU Tao, JIN Xiuliang
    2024, 36(1):  18-31.  DOI: 10.3969/j.issn.1004-1524.20230284
    Abstract ( 179 )   HTML ( 10 )   PDF (2239KB) ( 315 )  

    In order to explore the effect of multispectral data of unmanned aerial vehicles (UAV) acquired at different time on the estimation of canopy chlorophyll density (CCD) of maize, the UAV multispectral observation experiment was conducted at 10:00—10:59, 11:00—11:59, 13:00—13:59, 14:00—14:59 at the tasseling silking stage, blister stage, milk stage and dough stage, respectively. The PROSAIL model simulation results and the measured CCD data were combined to analyze the changes of the typical vegetation indexes at different time of the day and the difference between the CCD estimation results. The results showed that both the canopy reflectance and the value of vegetation indexes strongly correlated with the measured CCD changed with the time on the same day. The most obvious change of reflectance was found in the near-infrared band on the same day. The vegetation index value decreased as it approached 12:00 in the noon, whereas the value of vegetation indexes simulated by PROSAIL model at different time of the day showed little difference. The correlation between the same vegetation index obtained at different observation time and the measured CCD was different on the same day, and the difference between growth stages and indexes was not consistent; while the correlation between the same simulated vegetation index and CCD was not obvious at different time on the same day. CCD estimation models based on UAV data at different observation time could achieve good accuracy at different growth stages, but the estimation results at different observation time were different, with the lowest determination coefficient (R2) of 0.53 and the highest R2 of 0.80. These results showed that the traditional spectral data acquisition time range (10:00—14:00) had an impact on CCD estimation of maize, and the closer to the noon (12:00), the higher the estimation accuracy. These results provided basic support for the accurate CCD estimation of crops.

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    Phylogenetic relationship and genetic differentiation of maize landraces revealed by genome-wide SNP developed by genotyping-by-sequencing in Daliangshan Mountain area, China
    LENG Yifeng, LUO Fan, CHEN Congshun, DING Xin, CAI Guangze
    2024, 36(1):  32-47.  DOI: 10.3969/j.issn.1004-1524.20230012
    Abstract ( 164 )   HTML ( 13 )   PDF (6063KB) ( 398 )  

    Maize landraces are important source of maize breeding germplasm, with diverse types and outstanding field performance for some traits, often containing excellent resistance genes that can be utilized in breeding. Due to the unique geographical location and light-thermal resources of Daliangshan Mountain area, many maize landraces with distinctive characteristics had been accumulated here in maize production. However, the lack of systematic genetic research on these varieties had resulted in slow progress in maize breeding and utilization in this region. In this study, the genotyping-by-sequencing (GBS) technique was used to conduct phylogenetic and genetic differentiation analyses on 360 maize landraces collected from different regions of Daliangshan Mountain area. Through high-throughput sequencing analysis, 250.99 GB of effective data was generated, with a total of 1 659 033 712 clean reads from all samples, with an average read count of 4 608 427 and an average raw base of 0.70 GB and clean base of 0.67 GB. The average Q20 value of all samples was greater than or equal to 94.57%, the average Q30 value was greater than or equal to 87.14%, and the average GC content was 48.20%. After SNP calling and filtering, 124 342 single nucleotide polymorphism (SNP) loci and 32 063 insertion-deletion (InDel) loci (including 15 738 insertion loci and 16 325 deletion loci) were obtained. The neighbor-joining method was used to construct a phylogenetic tree, which clearly divided the 360 maize landraces into 2 major groups, A and B, supporting the two-group theory of maize heterosis. Principal component analysis also showed that the 2 groups were distantly related and presented genetic differentiation. Further population structure analysis divided the 2 major groups into 9 subgroups. The F-statistics (Fst) value between group A and group B was 0.462 2, and the nucleotide diversity (π) of group A was higher than that of group B. In the selective sweep analysis, 96 genomic regions were identified, and 418 genes were further filtrated from the top 5% of the selective regions. Gene ontology enrichment analysis showed that the candidate genes enriched in the selective regions were related to stress response, such as cold response genes, drought response genes, and pathogen response genes. The results of the study provided a reference for the conservation and genetic improvement of maize landraces in Daliangshan Mountain area.

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    Animal Science
    Estimation of genetic parameters of body size and body weight at different growth stages of Luzhong mutton sheep
    YANG Cunming, LIU Jing, ZHANG Menghua, ZHANG Xiaoxue, LIU Guifen, HE Junmin, MAO Jingyi, LI Xue, TANG Li, ZHANG Wenjing, PAN Linxiang, TIAN Kechuan, HUANG Xixia
    2024, 36(1):  48-57.  DOI: 10.3969/j.issn.1004-1524.20230138
    Abstract ( 171 )   HTML ( 4 )   PDF (691KB) ( 268 )  

    The objective of this study is to estimate the genetic parameters of body size and body weight of Luzhong mutton sheep at different growth stages, and to analyze the genetic rules between various traits, in order to provide a theoretical basis for the breeding planning of Luzhong mutton sheep. A total of 15 040 Luzhong mutton sheep body size and body weight data were collected from 2015 to 2019, and the body weight, body height, body length, chest circumference, chest width, chest depth, and cannon circumference traits of firstborn, 3 months, 6 months, one year and adult stage were taken as the research objects, and AI-REML combined with EM algorithm in DMU software was used to cooperate with animal models, taking birth year, birth season and sex as fixed effects, and additive effect and maternal effect as random effects, to estimate the heritability and genetic correlation of each trait. The heritability of Luzhong meat sheep weight from fristborn to adult was 0.18-0.51, the heritability of body height was 0.15-0.60, the heritability of body length was 0.09-0.42, the heritability of chest circumference was 0.11-0.43, the heritability of chest width was 0.05-0.39, the heritability of chest depth was 0.06-0.21, the heritability of cannon circumference was 0.11-0.59, and the maternal heritability of the firstborn to 6 months was 0.15-0.26, the genetic correlation coefficient between traits at different stages was -0.52-0.98, and the phenotypic correlation was -0.05-0.95. The genetic correlation coefficient of body weight between different growth stages was -0.10-0.87, and the phenotype correlation was 0.01-0.91. The maternal genetic effect had a high influence on the early growth traits of Luzhong meat sheep, and with the gradual weakening of the maternal genetic effect of growth, the heritability of body size and body weight of one year old was the highest in this study, and there was a genetic correlation between body size and body weight at different growth stages. The genetic correlation coefficient between 6 months old and one year old was the highest among the growth stages.

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    Histological study on the effect of high temperature stress on liver tissue in Larimichthys polyactis
    HE Yu, LIU Feng, ZHANG Tianle, LOU Bao, WEI Fuliang, YE Ting
    2024, 36(1):  58-66.  DOI: 10.3969/j.issn.1004-1524.20230230
    Abstract ( 180 )   HTML ( 11 )   PDF (5296KB) ( 414 )  

    In order to explore the effect of high temperature stress on the liver tissue structure and apoptosis of Larimichthys polyactis, the rearing temperature was raised from 15 ℃ to 31 ℃ by altering the water temperature in the tank at a constant rate of 2 ℃·h-1, and maintained to 96 h, recorded as the test group. At the same time, a control group rearing under the temperature of 15 ℃ was set. At the 0, 6, 12, 24, 48, 72, 96 h after the rearing temperature of the test group reached 31 ℃, the liver tissue of the test and control group were collected to investigate the histological changes and apoptosis of the liver tissues through the methods of tissue sections and terminal-deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL), respectively. The results showed that the liver morphology of microstructure and ultrastructure were similar to those of the control group after 0 h treatment at 31 ℃, and there was no significant change. However, with the extension of high temperature treatment time, liver tissue damage gradually worsened. After 96 h of treatment, the liver showed serious damage, including edema, disappearance or even dissolution of nuclei, local necrosis of liver tissue, and destruction of the original structure of liver cells. In addition, with the continuation of high temperature stress, the phenomenon of liver cell apoptosis gradually aggravated. The results showed that high temperature stress caused a certain degree of damage to the liver tissue of L. polyactis and induced a large number of liver cell apoptosis. The results of the present study clarified the effect of high temperature stress on the liver tissue of L. polyactis, which is of great significance for the rearing management of L. polyactis to avoid the occurrence of liver disease.

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    Natural products screening with resistance to honeybee viruses
    HUANG Zhichu, ZHOU Nihong, LIU Yao, ZHENG Huoqing
    2024, 36(1):  67-74.  DOI: 10.3969/j.issn.1004-1524.20230190
    Abstract ( 143 )   HTML ( 9 )   PDF (1149KB) ( 266 )  

    In order to identify natural products with anti-honeybee virus effects, 9 natural products (thymol, artemisinin, catalpol, piperine, theobromine, amygdalin, caffeic acid, hesperidin and ρ-coumaric acid) were tested in lab conditions. RT-PCR was used to measure the inhibitory effect of the 9 natural products on honeybee viruses after 7 days. Additionally, the effects of the natural products on the expression of honeybee immunity-related genes were also determined. The results of the exogenous inoculation of Israeli acute paralysis virus (IAPV) virus suggest that feeding honeybees with 10 μmol·L-1 thymol for 7 days can significantly reduce the viral load of IAPV and Sacbrood virus (SBV). Without external vaccination of the virus, after feeding with 10 μmol · L-1 thymol for 7 days, the viral load of IAPV and SBV carried by honeybees was significantly reduced compared with the control group, and the transcription level of vitellogenin gene was significantly increased. After screening, it was found that honeybees fed with 10 μmol · L-1 thymol can achieve good antiviral effects after 7 days, and thymol can become a potential component in the development of anti-honeybee virus drugs.

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    Horticultural Science
    Evaluation on nutritional value of Flammulina filiformis cultivation substrate with different cultivation time
    WANG Fei, HAN Yujie, FANG Yi, XIANG Hai, CHANG Xiao, ZHONG Rongzhen
    2024, 36(1):  75-83.  DOI: 10.3969/j.issn.1004-1524.20230059
    Abstract ( 158 )   HTML ( 9 )   PDF (803KB) ( 344 )  

    In order to evaluate the effect of different cultivation time on the nutritional value of Flammulina filiformis cultivation substrate, the samples of the cultivation substrate of F. filiformis were collected on the 0th, 9th, 14th, 18th, 22nd, 29th, 36th, and 51st days of cultivation.The contents of nutritional ingredients and amino acid (AA) composition of these samples were determined. Besides, the nylon bag technique was adopted to determine the dry matter degradability after in vivo digestion in the rumen for 0, 2, 4, 8, 12, 24, 48, 72 h, respectively, as well as the degradation rate of nutritional ingredients after in vivo digestion for 24, 48 h, respecitvely. It was shown that, compared with the substrate before cultivation, the contents of crude protein, essential amino acids and total amino acids in the substrate cultivated for 29 d were significantly (P<0.05) increased to 8.96%, 0.47%, 1.01%, respectively, yet the aicd detergent lignin content was significantly decreased to the lowest level (7.72%) under the experiement conditions. For the substrate cultivated for 51 d, the hemicellulose content was the highest (31.37%), and the dry matter degradability was relatively high. In general, F. filiformis cultivation substrate could be used as the feed resource for ruminants, and the optimized cultivation time was 29 d.

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    Development of SSR molecular markers and application of fingerprint database based on whole genome of Stropharia rugosoannulata
    LI Xuesong, SUN Dafeng, LIU Shaoxiong, ZHANG Junbo, YUE Wansong, LI Jianying, HUA Rong
    2024, 36(1):  84-93.  DOI: 10.3969/j.issn.1004-1524.20221149
    Abstract ( 143 )   HTML ( 6 )   PDF (1048KB) ( 381 )  

    Based on experimental validation and bioinformatics methods, SSR analysis result were exploited from the genomic of Stropharia rugosoannulata and 13 strains. In the meantime, 100 SSR were selected for primer design and experimental verification. In order to provide a theoretical basis for the germplasm resources identification, variety selection and molecular marker-assisted breeding of S. rugosoannulata. The results showed that a total of 2 124 sequences of 6 SSR types were retrieved. The main type of trinucleotide (P3) was 704, accounting for 33.15% of the total SSR number. Pentanucleotide and hexanucleotide repeat types were more, the total number accounted for 14.59% of the total SSR number. 16 pairs of primers were obtained by verification test, three primers with high pair property, repeatability and specificity were obtained from the validation experiment, which were DQGSSR020, DQGSSR031 and DQGSSR077. At the same time, the DNA fingerprint coding of 16 pairs of primers was constructed and the 32-digit molecular fingerprint database codes of 13 S. rugosoannulata strains were obtained.

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    Identification and expression analysis of sucrose transporter SUT family in watermelon in fruit development and stress responses
    SHOU Weisong, WANG Duo, SHEN Jia, XU Xinyang, ZHANG Yuejian, HE Yanjun
    2024, 36(1):  94-102.  DOI: 10.3969/j.issn.1004-1524.20230188
    Abstract ( 241 )   HTML ( 13 )   PDF (1233KB) ( 399 )  

    Sucrose transporter (SUTs) gene, as the main active transport vector of sucrose, plays a key role in the transport of allotment assimilates from source tissue to sink tissue. Although the characteristics and biological functions of SUTs have been well studied in higher plants, the SUT gene family has not been identified in watermelon. In this study, four ClSUT genes were identified in watermelon genome, and their gene and protein structure, conserved motifs, subcellular localization, chromosome distribution, evolutionary relationships and promoter cis-elements were comprehensively analyzed. Gene structure analysis showed that ClSUT3 and ClSUT4 grouped into subgroup Ⅰ and each only contained one intron. All ClSUT proteins were predicted to be localized in the plasma membrane. All ClSUT proteins contain a conserved MFS domain corresponding to five motifs. Phylogenetic analysis revealed that SUT proteins in Arabidopsis, rice and watermelon were divided into 5 subgroups. ClSUTs in watermelon have more closely relationship with SUT proteins in Arabidopsis and were clustered in subgroup Ⅰ, Ⅱ and Ⅴ. In addition, We also identified several functional cis-elements related to hormone and stress response in the watermelon ClSUT gene promoter. And transcriptome data indicated that watermelon ClSUT genes were variously expressed in response to osmotic and salt stress, which may be involved in watermelon responses to these stresses.

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    Genetic diversity analysis and fingerprint construction of Dading bitter gourd germplasm resources
    YANG Tianwen, WANG Jing, LI Jiong, XU Binqi, CHENG Jiaowen, HONG Yu, CAO Yi, CUI Junjie
    2024, 36(1):  103-114.  DOI: 10.3969/j.issn.1004-1524.20230063
    Abstract ( 197 )   HTML ( 7 )   PDF (12662KB) ( 407 )  

    To achieve rapid and accurate identification of the germplasm resources of the Dading bitter gourd, 26 Dading bitter gourd germplasm resources including the main cultivars were selected as materials, cluster analysis and fingerprint construction were carried out by screening the high polymorphism simple sequence repeat (SSR) core markers. The results showed that among the 6 SSR motif unit types in the bitter gourd genome, the polymorphism of dinucleotide SSR markers was the best, which was suitable for further screening of SSR core markers. At the genetic similarity coefficient of 0.80, 40 SSR markers clustered the 26 Dading bitter gourd germplasm resources into 3 main groups. The first group contained 18 germplasm resources, and the fruit shape was mainly short conical. The second group contained 5 germplasm resources, and the fruit shape was mainly long conical. The third group contained 3 germplasm resources, consisting of materials with fruits similar to those of Dading bitter gourds but containing non-Dading bitter gourd germplasm backgrounds. The 4 SSR core markers, MC05_69594, MC04_50530, MC06_87314 and MC10_146038, were used to effectively identify 26 Dading bitter gourd germplasm resources, and a unique QR code fingerprint was established for each Dading bitter gourd germplasm, among which No. 1 and No. 3 were suspected to be similar materials. Taken together, the fingerprint of Dading bitter gourd could provide a scientific basis for the germplasm identification and breeding utilization for Dading bitter gourd.

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    Characteristics of endophyte’s community changes of Paeonia suffruticosa cv. Lu He Hong fine root in different plant ages
    QIAO Hongyong, YUAN Tao, ZHAO Xinyong, YANG Huiyan
    2024, 36(1):  115-126.  DOI: 10.3969/j.issn.1004-1524.20230239
    Abstract ( 123 )   HTML ( 5 )   PDF (4187KB) ( 402 )  

    By comparing and analyzing the endophyte’s community structure, functional characteristics and network relationship in fine roots of tree peony variety Lu He Hong in different plant ages, this study provided theoretical support for exploring the effect of plant age on endophytes in tree peony and enriching endophytes resources in tree peony. In this study, the samples of Lu He Hong fine roots (≤2 mm) aged 5 (ART1), 12 (ART2), 20 (ART3) and 30 years (ART4) were used to carry out relevant bioinformatics analysis based on 16S rDNA and ITS high-throughput sequencing technology. The results showed that 97% sequences of endophytic bacteria and endophytic fungi from Lu He Hong fine roots were clustered into 4 994 and 1 485 operational taxonomic units (OTUs), respectively, and the plant age did not affect the abundance and diversity of endophytic communities. Cyanobacteria/Chloroplast, Proteobacteria, Actinobacteria, Bacteroidetes, Acidobacteria were the dominant bacterial groups of Lu He Hong fine root endophytes. After more than 20 years of plant age, Actinobacteria and Bacteroidetes decreased significantly, while Acidobacteria gradually increased. Ascomycota was the dominant endophytic fungi in the fine roots of Lu He Hong, which reached 93.99%-96.14%. The fine root cellulose content, POD and CAT activity increased significantly with the increase of plant age, and significantly affected the community structure of endophytic bacteria. PICRUSt2 and FUNGuild results showed that the function of endophytic bacteria was mainly focused on metabolism with little change, and plant age mainly affected the ecological function of endophytic fungi. The pathotroph fungi increased significantly in Lu He Hong fine roots at plant age of more than 12 years, which was easy to be infected with diseases. MENA analysis showed that the increase of plant age aggravated the competition among endophytes and weakened their network connectivity. In conclusion, plant age causes different degrees of changes in the endophyte’s community of tree peony fine roots, and the harmful endophytes increase after 20 years of plant age, which is prone to disease. The results of this study can lay a theoretical foundation for the preventing diseases of elder tree peony.

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    Genetic diversity of Rhododendron huadingense based on SARP molecular marker
    WU Qian, TANG Ziyi, TIAN Shengye, HE Haiye, PAN Weiwei, WANG Junfeng, BAO Honghua, ZHANG Huijuan, JIANG Ming
    2024, 36(1):  127-133.  DOI: 10.3969/j.issn.1004-1524.20221828
    Abstract ( 156 )   HTML ( 3 )   PDF (9403KB) ( 403 )  

    Rhododendron huadingense is a rare and endangered wild plant species endemic to Zhejiang Province, China, with narrow distribution and very few individuals, thus this species needs to be protected. In this study, leaves of R. huadingense from different populations were collected, and SRAP (sequence related amplified polymorphism) molecular marker technology was applied to study its genetic diversity. Fifty genomic DNA samples were used for PCR amplification. The results indicated 8 pairs of primers with high levels of polymorphism, clear bands, and high reliability were screened from 140 primer pairs. Totally, 263 bands were detected, including 261 polymorphic sites, and the percentage of the polymorphic band reached 99.24%. On species level, the number of observed alleles (Na), number of effective alleles (Ne), Nei’s gene diversity (H), and Shannon’s information index (I) were 1.992 4, 1.340 1, 0.221 5, and 0.357 9, respectively, revealing its high genetic diversity on species level. On population level, the coefficient of genetic differentiation (Gst) and gene flow (Nm) were 0.459 6 and 0.587 8, respectively, indicating genetic differentiation and gene flow have occurred among populations, and most of the variation existed within populations. UPGMA results showed samples from 5 populations could be divided into 5 groups, which were consistent with principal component analysis results. Studies on genetic diversity of R. huadingense provided insights on protection and utilization of this rare plant species.

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    Yield and quality characteristics of passion fruit under different cultivation modes in Fujian, China
    LIU Zhicheng, YAN Liangwen, CHEN Yaoyao, OU Xueting
    2024, 36(1):  134-147.  DOI: 10.3969/j.issn.1004-1524.20221826
    Abstract ( 194 )   HTML ( 9 )   PDF (1156KB) ( 475 )  

    To explored the characteristics of field growth time, yield and quality related indexes of passion fruit under multi-tiered fruiting vine cultivation mode and single-tier fruiting vine cultivation mode, from 2020 to 2021, a field production trial of passion fruit was conducted. The main planting variety of the purple-fruited passion fruit Tainong No. 1 and the yellow-fruited passion fruit Balewei were selected as materials. The study observed and measured the flowering, fruiting, yield, and quality-related indicators of passion fruit under two cultivation modes. It also analyzed the correlation between fruit quality indicators and meteorological factors such as temperature and light during the fruit ripening period. The results showed that: 1) Compared with the multi-tiered fruiting vine cultivation mode, the date for passion fruit plants to start budding, flowering, and fruit setting was later in the single-tier fruiting vine cultivation mode. The time required for fruit development and maturation was shortened, the flower and fruit setting rates significantly increased, and the coefficients of variation of the budding period, flowering rate, fruit development and maturation time, and fruit setting rate were all relatively low. The time from planting to harvest for Tainong No. 1 and Balewei was shortened by 69-71 d and 51-58 d, respectively, compared to the multi-tiered fruiting vine cultivation mode. 2) In terms of the total actual yield and its components, the single-tier fruiting vine cultivation mode resulted in significantly lower individual plant yields compared to the multi-tiered fruiting vine cultivation mode. All of its yield came from the primary vine, whereas the individual plant yield in the multi-tiered fruiting vine cultivation mode was composed of yields from the main vine, primary vine, secondary vine, and tertiary vine, and the proportions of yields from each level of vine varied. Compared with the multi-tiered fruiting vine cultivation mode, the single-tier fruiting vine cultivation mode led to a significant reduction in the number of fruit per plant, but a significant increase in the average fruit weight and the proportion of medium to large-sized fruit. 3) When compared with the multi-tiered fruiting vine cultivation mode, the single-tier fruiting vine cultivation mode led to a significant decrease in the total acid content of the fruit, whereas the fruit skin weight, juice volume, edibility rate, total sugar, and sugar-acid ratio increased. Moreover, the coefficients of variation of the values of the 7 indicators of fruit quality were relatively low, and there was a significant correlation between meteorological factors such as temperature and light and fruit quality. In summary, the passion fruit under the single-tier fruiting vine cultivation mode shortened the field growth time and exhibited superior characteristics in terms of flowering, fruiting, yield, and quality-related indicators compared to the multi-tiered fruiting vine cultivation mode, with relatively stable coefficients of variation for the various indicators.

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    Comparison of tolerance to high temperature of excellent strains of Sargassum fusiforme
    ZUO Xiaojie, WU Mingjiang, LUO Lin, MA Zengling, PANG Guanfeng, CHEN Binbin
    2024, 36(1):  148-155.  DOI: 10.3969/j.issn.1004-1524.20221808
    Abstract ( 167 )   HTML ( 6 )   PDF (2752KB) ( 443 )  

    Three excellent strains of Sargassum fusiforme and wild-type S. fusiforme were cultured at 25 ℃ to compare the growth status of S. fusiforme under high temperature conditions, and to provided data support for the screening of high temperature resistance strains. The results showed that long-term high temperature culture could reduce the photosynthetic performance of each strain of S. fusiforme, inhibit the activity of nitrate nitrogen absorption and assimilation related enzymes, reduce the content of soluble protein and soluble carbohydrate, enhance its dark respiration rate, and ultimately inhibit the growth of algae. Under high temperature condition, compared with other strains, the maximum electron transfer rate and saturated light intensity oxygen release rate of spindle vesicle type S. fusiforme were the highest, the peroxidase activity was the highest and increased significantly, while its malondialdehyde content did not increase significantly. In general, spindle vesicle type of S. fusiforme was less affected by high temperature and had strong tolerance. Spindle vesicle type of S. fusiforme could be popularized in actual breeding production, and the research results could provide a basis for subsequent variety improvement.

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    Plant Protection
    Phyllosphere microecology of brown spot and healthy tobacco leaves after application of myclobutanil
    ZHANG Yi, WANG Feng, CAI Liuti, WANG Hancheng, XIONG Jing, CHEN Xingjiang
    2024, 36(1):  156-167.  DOI: 10.3969/j.issn.1004-1524.20230242
    Abstract ( 161 )   HTML ( 4 )   PDF (3332KB) ( 298 )  

    To understand the differences in the structure and metabolic functions of the phyllosphere microorganisms of tobacco leaves in the control of brown spot by myclobutanil, Illumina high-throughput sequencing and Biolog-ECO technology was conducted to investigate the structure and diversity of phyllospheric microorganisms community and their metabolic function. The results showed that the phyllosphere fungi of the diseased and healthy tobacco leaves included Alternaria, Archaeorhizomyces, Boeremia, Cladosporium, etc., of which Alternaria was the dominant genus; the bacteria were Kosakonia, Pantoea and Methylobacterium. Among them, Kosakonia is the dominant genus, and the relative abundance of the Alternaria and Kosakonia was higher in diseased tobacco leaves than in healthy tobacco leaves. The relative abundance of Alternaria and Cladosporium decreased, while the relative abundance of Epicoccum and Didymella decreased and then increased when treated with 60 mL 15% myclobutanil microemulsion per 667 m2 for 5, 10 and 15 d; the relative abundance of phyllosphere fungi in healthy tobacco leaves decreased in the genera Alternaria, Symmetrospora, and Didymella. The relative abundance of phyllosphere Kosakonia in diseased tobacco leaves decreased at 5 d of application and increased at 10 and 15 d, while the opposite was true for Pseudomonas. The relative abundance of Pantoea and Pseudomonas decreased at 5 and 10 d and increased at 15 d. The phyllospheric microorganism metabolic activity of brown spot-infected leaves was lower than that of healthy tobacco leaves. The metabolism of carbon sources other than α-butyric acid, L-threonine and 4-hydroxybenzoic acid by phyllosphere microorganisms was strong. The metabolic activity of phyllosphere microorganisms in diseased and healthy tobacco leaves were reduced after myclobutanil treatment, and the metabolic activity of phyllospheric microorganisms gradually recovered with the increasing of application time. The results of the study revealed the differences in phyllosphere microecological changes between diseased and healthy tobacco leaves during different periods of myclobutanil application, providing a reference for the scientific application of myclobutanil in the control of tobacco brown spot.

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    Identification of pathogen causing gummy stem blight of Cucumis melo and Trichosanthes kirilowii in Anhui Province and screening of effective fungicides
    XIA Zhijie, ZHANG Lei, SONG Jianghua, FU Min, ZHANG Lixin
    2024, 36(1):  168-176.  DOI: 10.3969/j.issn.1004-1524.20230146
    Abstract ( 148 )   HTML ( 9 )   PDF (3676KB) ( 258 )  

    To identify the pathogen causing gummy stem blight of Cucumis melo and Trichosanthes kirilowii, and screen effective fungicides for the management of this disease, symptomatic samples were collected from the fields located in Shucheng County and Chaohu City, Anhui Province in 2021 and 2022. Pure cultures were obtained consistently using tissue separation method, and five representative isolates were selected for morphological observation and molecular identification. Partial fragments of internal transcribed spacer (ITS) and large subunit (LSU) regions, beta-tublin 2 (TUB2) genes of the isolates were amplified and sequenced. Pathogenicity assays were carried out to confirm Koch’s postulates. The pathogen was finally identified as Stagonosporopsis citrulli based on morphological characteristics and multi-loci sequence analysis. The indoor toxicity test results indicated that boscalid and prochloraz exhibited relatively higher inhibitory effects among seven fungicides, with both EC50 ranging from 0.010 6 to 0.043 8 μg·mL-1. The two fungicides could be preferentially utilized in rotation to control the disease caused by S. citrulli.

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    Environmental Science
    Effect of nitrogen transforming flora on nitrogen retention in aerobic composting of cattle manure
    WANG Jiali, WANG Ziyu, MA Yongqi, TANG Defu, SUN Likun
    2024, 36(1):  177-186.  DOI: 10.3969/j.issn.1004-1524.20221466
    Abstract ( 156 )   HTML ( 6 )   PDF (1127KB) ( 295 )  

    To investigate the effect of nitrogen transforming flora on the aerobic composting of cattle manure, the experiment was conducted with cattle manure and maize straw as raw materials, and the addition of self-developed complex microbial flora was used as the inoculation group, and the addition of the same volume of sterile water was taken as the control group. The results showed that addition of 10 mL·kg-1 self-developed microbial flora for aerobic composting of cattle manure increased the highest temperature of the composting by 5.8 ℃, shortened the time to delayed warming period, entered the high temperature (>50 ℃) period 4 days earlier, and lasted 4 days longer in the high temperature period, thus accelerated the decomposition of cattle manure. At the same time, the addition of microbial flora was able to fix nitrogen nutrients in the pile, as the total nitrogen content was increased by 8.14% and the ammonia emission was cut by 29.02%.

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    Influence of no tillage with high stubble on macrofauna community of black soil
    MA Bingzeng, JIANG Yunfeng, YAN Ting, LIU Junnan
    2024, 36(1):  187-195.  DOI: 10.3969/j.issn.1004-1524.20230161
    Abstract ( 155 )   HTML ( 6 )   PDF (1141KB) ( 345 )  

    In order to clarify the effect of no tillage with high stubble on soil macrofauna community, soil macrofauna community under the treatments of conventional tillage (CT), no tillage (NT) and no tillage with high stubble (SS) was investigated in the research and development base of conservation tillage in Lishu County, Jilin Province, in spring, summer and autumn. Soil macrofauna were obtained belonging to 2 phyla 4 classes 14 orders. In each treatment, Enchytraeidae was the dominant group, while Coleoptera, Geophilomorpha, Diptera larvae and Formicidae were common. In spring, the density of soil macrofauna under NT treatment was significantly (P<0.05) higher than that under CT by 2.8-fold. In summer, the density of soil macrofauna under SS treatment was significantly higher than that under CT by 1.8-fold. In antumn, the density of soil macrofauna under NT and SS was significantly higher than that under CT by 1.1-fold and 1.3-fold, respectively. The density of soil macrofauna was low in spring and high in autumn. The community composition of soil macrofauna under SS treatment varied from that under CT treatment, especially in summer and autumn, but the difference between SS and NT was not significant. The density of Enchytraeidae was significantly increased under SS treatment in summer and autumn compared with that under CT. In general, no tillage with high stubble was beneficial to increase the density of soil macrofauna.

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    Food Science
    Effect of different hollow packaging on storage and transportation quality of bayberry harvested in rainy days
    ZONG Zihao, HE Dingsheng, NIU Ben, HUANG Jun, FANG Xiangjun, WU Weijie, CHEN Hangjun, GAO Haiyan
    2024, 36(1):  196-204.  DOI: 10.3969/j.issn.1004-1524.20230181
    Abstract ( 151 )   HTML ( 8 )   PDF (2449KB) ( 453 )  

    In order to solve the problems of water accumulation on the surface of bayberry harvested in rainy days, and the deterioration of appearance and nutritional quality of fresh fruits due to mechanical damage accumulation caused by vibration during storage and transportation, in this study, we developed a new hollowed-out packaging, and traditional basket packaging for fruits was used as a control group to explore the effect of different hollowed-out forms on the quality of Biqi bayberry. The results showed that the full hollowed-out packaging method was beneficial to reduce the pre-cooling time after picking of fruits, and the core temperature reached 4 ℃ after 48 min of pre-cooling. The non-hollowing packaging method effectively reduced the weight loss rate of fresh fruits during transportation, and the weight loss rate was maintained at 13.49% at 10 days of storage. The semi-hollowing packaging method had significant advantages in maintaining good appearance and nutritional quality, including the contents of titrable acids, soluble solids, vitamin C, total phenols, and anthocyanin. At the same time, the semi-hollowing packaging method can better inhibit the rise of malondialdehyde content in fresh fruits during storage and transportation. By comprehensive analysis of the appearance and nutritional quality of fruits during storage, the semi-hollowing group had a better effect among all treatments.

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    Effects of drying methods on quality of solid drinks made from mulberry leaves and barbary wolfberry fruits and its taste optimization
    CHEN Henghui, WANG Junfeng, HAN Yanchao, CHEN Huizhi, WU Weijie, DING Yuting, TONG Chuan, GAO Haiyan
    2024, 36(1):  205-214.  DOI: 10.3969/j.issn.1004-1524.20230414
    Abstract ( 160 )   HTML ( 6 )   PDF (2142KB) ( 334 )  

    In this study, the influence of drying methods on the quality of solid drinks prepared by mulberry (Morus alba L. ) leaves and barbary wolfberry (Lycium barbarum) fruits was investigated. The mulberry leaves and barbary wolfberry fruits were used as the basic raw materials, and the solid drinks were dried by three methods including microwave drying, forced air drying and vacuum freeze drying. The changes in the texture, color and nutritional quality of the solid drinks powder after drying were analyzed. The results of physical properties showed that vacuum freeze drying had good stability, homogeneity and compressibility, while the forced air drying showed the best accumulation and flowability. The powder dried by vacuum freeze drying had the highest total sugar content, the highest brightness and the shortest moisture sinking time. Given the moisture absorption characteristics and microstructure, the quality of solid drinks powder prepared by vacuum freeze drying was better than the others. In order to improve the flavor of the soild drinks, the response surface test was conducted. The results showed that, the taste of the solid drinks was the optimal with the addition of xylitol (mass fraction of 27.30%), stevia glycoside (mass fraction of 3.67%) and malic acid (mass fraction of 0.11%).

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    Biosystems Engineering
    Plant leaf disease identification based on contrastive learning
    YANG Xinyu, FENG Quan, ZHANG Jianhua, YANG Sen
    2024, 36(1):  215-224.  DOI: 10.3969/j.issn.1004-1524.20230093
    Abstract ( 233 )   HTML ( 10 )   PDF (3028KB) ( 329 )  

    At present, the recognition of plant disease via image processing mostly relies on the manually labeled convolutional neural network. However, the self-monitoring contrastive learning could achieve independent learning without relying on labels and large amounts of data. In view of this advantage, the effect of four contrastive learning methods, MoCo-v2, DeepCluster-v2, SwAV and BYOL, on the identification of plant leaf diseases were compared by setting different experimental conditions on the open-source dataset of PlantVillage and the self-built cotton disease dataset. The ResNet50 encoder trained by four contrastive learning methods was tested for disease identification both under Linear and Finetune modes, and the feasibility of the contrastive learning methods in identifying plant leaf diseases was evaluated. It was shown that the average accuracy under Finetune mode on the PlantVillage dataset was higher than that under Linear mode, and the highest identificaiton accuracy of the encoders trained by the four methods reached 99.83%. DeepCluster-v2 and BYOL had the highest identification rate under Finetune mode, both of which were 99.87%. On the self-built cotton disease dataset, the performance under Finetune mode was poorer than that under Linear mode, and the highest idenficaiton accuracy of DeepCluster-v2 under Linear mode was 98.86%. Overall, the disease identification rate based on contrastive learning method was superior to the supervised models, demonstrating good application prospects in plant leaf disease identification.

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    Agricultural Economy and Development
    An “inverted U-shaped” relationship between agricultural insurance coverage level and agricultural income: from perspective of agricultural industry integration
    WEI Chao, CHEN Shengwei, NIU Hao, LI Zheng
    2024, 36(1):  225-234.  DOI: 10.3969/j.issn.1004-1524.20230213
    Abstract ( 187 )   HTML ( 13 )   PDF (983KB) ( 229 )  

    Based on the panel data of 30 provinces in China (excluding Hong Kong, Macao, Taiwan and Xizang) from 2008 to 2020, the fixed effect model, Ⅳ-2SLS method and other tools were adopted to explore the impact of agricultural insurance coverage level on agricultural income and its mechanism path. It was found that there was a significant and robust “inverted U-shaped” relationship between the agricultural insurance coverage level and agricultural income, with a turning point value of 83.09% for the agricultural insurance coverage level. The agricultural industry integration played a part of the mesomeric effect in the mechanism of agricultural insurance coverage level promoting agricultural income increase, with the mesomeric effect accounting for 82.24%. Thus, it was proposed to accelerate the innovation of agricultural insurance products and the expansion of insurance liabilities, promote the “addition, upgrading, and expansion” of agricultural insurance, and provide comprehensive risk protection for increasing agricultural income.

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  • 2 2020-12-04
  • 1 2020-12-04
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