Characteristics of endophyte’s community changes of Paeonia suffruticosa cv. Lu He Hong fine root in different plant ages

doi:10.3969/j.issn.1004-1524.20230239

Acta Agriculturae Zhejiangensis ›› 2024, Vol. 36 ›› Issue (1): 115-126.DOI: 10.3969/j.issn.1004-1524.20230239

• Horticultural Science • Previous Articles Next Articles

Characteristics of endophyte’s community changes of Paeonia suffruticosa cv. Lu He Hong fine root in different plant ages

QIAO Hongyong¹(), YUAN Tao¹^,^*(), ZHAO Xinyong², YANG Huiyan²

1. Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, School of Landscape Architecture, Beijing Forestry University, Beijing 100083, China
2. Caozhou Garden, Heze 274000, Shandong, China

Received:2023-02-28 Online:2024-01-25 Published:2024-02-18

Abstract

Abstract:

By comparing and analyzing the endophyte’s community structure, functional characteristics and network relationship in fine roots of tree peony variety Lu He Hong in different plant ages, this study provided theoretical support for exploring the effect of plant age on endophytes in tree peony and enriching endophytes resources in tree peony. In this study, the samples of Lu He Hong fine roots (≤2 mm) aged 5 (ART1), 12 (ART2), 20 (ART3) and 30 years (ART4) were used to carry out relevant bioinformatics analysis based on 16S rDNA and ITS high-throughput sequencing technology. The results showed that 97% sequences of endophytic bacteria and endophytic fungi from Lu He Hong fine roots were clustered into 4 994 and 1 485 operational taxonomic units (OTUs), respectively, and the plant age did not affect the abundance and diversity of endophytic communities. Cyanobacteria/Chloroplast, Proteobacteria, Actinobacteria, Bacteroidetes, Acidobacteria were the dominant bacterial groups of Lu He Hong fine root endophytes. After more than 20 years of plant age, Actinobacteria and Bacteroidetes decreased significantly, while Acidobacteria gradually increased. Ascomycota was the dominant endophytic fungi in the fine roots of Lu He Hong, which reached 93.99%-96.14%. The fine root cellulose content, POD and CAT activity increased significantly with the increase of plant age, and significantly affected the community structure of endophytic bacteria. PICRUSt2 and FUNGuild results showed that the function of endophytic bacteria was mainly focused on metabolism with little change, and plant age mainly affected the ecological function of endophytic fungi. The pathotroph fungi increased significantly in Lu He Hong fine roots at plant age of more than 12 years, which was easy to be infected with diseases. MENA analysis showed that the increase of plant age aggravated the competition among endophytes and weakened their network connectivity. In conclusion, plant age causes different degrees of changes in the endophyte’s community of tree peony fine roots, and the harmful endophytes increase after 20 years of plant age, which is prone to disease. The results of this study can lay a theoretical foundation for the preventing diseases of elder tree peony.

Key words: Paeonia suffruticosa, endophyte, plant age, community structure, function prediction, molecular network

CLC Number:

S685.11

QIAO Hongyong, YUAN Tao, ZHAO Xinyong, YANG Huiyan. Characteristics of endophyte’s community changes of Paeonia suffruticosa cv. Lu He Hong fine root in different plant ages[J]. Acta Agriculturae Zhejiangensis, 2024, 36(1): 115-126.

Figures/Tables 10

References 50

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株龄 Plant age	株高 Plant height/cm	东西冠幅 East-west crown width/cm	南北冠幅 North-south crown width/cm	基部分枝/个 Basal branching	基部最粗枝直径 Base coarsest branch diameter/cm	基部最细枝直径 Base most slender branch diameter/cm
ART1	46.83	61.33	60.33	7	1.20	0.60
ART2	85.33	104.33	99.67	7	3.10	1.58
ART3	108.33	109.33	101.33	6	4.00	2.17
ART4	120.00	118.57	112.86	4	4.66	2.66

株龄 Plant age	株高 Plant height/cm	东西冠幅 East-west crown width/cm	南北冠幅 North-south crown width/cm	基部分枝/个 Basal branching	基部最粗枝直径 Base coarsest branch diameter/cm	基部最细枝直径 Base most slender branch diameter/cm
ART1	46.83	61.33	60.33	7	1.20	0.60
ART2	85.33	104.33	99.67	7	3.10	1.58
ART3	108.33	109.33	101.33	6	4.00	2.17
ART4	120.00	118.57	112.86	4	4.66	2.66

株龄 Plant age	可溶性糖含量 Soluble sugar content/ (μg·g^-1)	纤维素含量 Cellulose content/ (μg·g^-1)	可溶性蛋白含量 Soluble protein content/ (mg·g^-1)	丙二醛含量 Malondialdehyde content/ (nmol·g^-1)	超氧化物歧化酶活性 SOD activity/ (U·mL^-1)	过氧化物酶活性 POD activity/ (U/mL^-1)	过氧化氢酶活性 CAT activity/ (U/mL^-1)	根系活力 Root vigor/ (mg·g^-1·h^-1)
ART1	61.52±8.11 b	1.08±0.03 c	9.07±0.18 b	3.91±0.45 c	102.66±4.60 c	6.53±1.04 d	4.68±0.27 d	1.45±0.05 b
ART2	70.83±5.07 a	1.54±0.23 b	10.30±0.85 a	5.78±0.73 ab	114.13±4.33 b	9.05±1.27 c	6.15±0.54 c	1.66±0.12 a
ART3	66.38±2.81 ab	1.58±0.12 b	10.54±0.90 a	6.20±0.60 a	153.15±5.07 a	11.19±0.76 b	8.72±0.71 b	1.76±0.14 a
ART4	43.16±7.18 c	2.33±0.19 a	8.41±0.91 b	5.24±0.77 b	85.81±4.88 d	12.80±0.97 a	10.73±0.69 a	1.52±0.10 b

株龄 Plant age	可溶性糖含量 Soluble sugar content/ (μg·g^-1)	纤维素含量 Cellulose content/ (μg·g^-1)	可溶性蛋白含量 Soluble protein content/ (mg·g^-1)	丙二醛含量 Malondialdehyde content/ (nmol·g^-1)	超氧化物歧化酶活性 SOD activity/ (U·mL^-1)	过氧化物酶活性 POD activity/ (U/mL^-1)	过氧化氢酶活性 CAT activity/ (U/mL^-1)	根系活力 Root vigor/ (mg·g^-1·h^-1)
ART1	61.52±8.11 b	1.08±0.03 c	9.07±0.18 b	3.91±0.45 c	102.66±4.60 c	6.53±1.04 d	4.68±0.27 d	1.45±0.05 b
ART2	70.83±5.07 a	1.54±0.23 b	10.30±0.85 a	5.78±0.73 ab	114.13±4.33 b	9.05±1.27 c	6.15±0.54 c	1.66±0.12 a
ART3	66.38±2.81 ab	1.58±0.12 b	10.54±0.90 a	6.20±0.60 a	153.15±5.07 a	11.19±0.76 b	8.72±0.71 b	1.76±0.14 a
ART4	43.16±7.18 c	2.33±0.19 a	8.41±0.91 b	5.24±0.77 b	85.81±4.88 d	12.80±0.97 a	10.73±0.69 a	1.52±0.10 b

内生微生物Endophytic microbial	株龄Plant age	Chao1	Shannon	Simpson
内生细菌Endophytic bacteria	ART1	1389.69±1307.46 a	1.19±0.88 a	1.70±0.70 a
	ART2	894.84±464.67 a	1.01±0.62 a	1.55±0.47 a
	ART3	1232.84±169.21 a	1.53±0.41 a	1.65±0.27 a
	ART4	995.38±305.50 a	1.00±0.50 a	1.33±0.21 a
内生真菌Endophytic fungi	ART1	592.29±154.31 a	2.74±0.39 a	6.07±1.41 a
	ART2	631.16±132.76 a	2.85±0.19 a	7.13±1.24 a
	ART3	544.28±75.53 a	2.71±0.31 a	7.44±2.09 a
	ART4	655.40±180.58 a	2.95±0.40 a	7.78±2.20 a

Characteristics of endophyte’s community changes of Paeonia suffruticosa cv. Lu He Hong fine root in different plant ages

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预测功能Prediction function	ART1	ART2	ART3	ART4
细胞过程Cellular processes	3.24±0.40 a	3.06±0.10 a	3.05±0.01 a	3.06±0.07 a
环境信息处理Environmental information processing	10.92±0.26 a	10.67±0.14 b	10.53±0.11 b	10.67±0.05 b
遗传信息处理Genetic information processing	18.31±0.76 a	18.15±0.41 a	18.44±0.34 a	18.11±0.26 a
人类疾病Human diseases	0.72±0.09 a	0.77±0.02 a	0.75±0.01 a	0.76±0.02 a
新陈代谢Metabolism	66.03±1.28 a	66.56±0.32 a	66.43±0.24 a	66.60±0.26 a
有机系统Organismal system	0.78±0.03 a	0.79±0.01 a	0.79±0.01 a	0.79±0.01 a
腐生营养型Saprotroph	21.19±9.30 a	5.67±7.79 b	8.19±14.77 b	4.77±4.60 b
病理营养型Pathotroph	46.42±19.18 b	76.15±10.10 a	84.57±19.34 a	84.60±10.10 a
共生营养型Symbiotroph	18.20±12.07 a	14.16±16.45 ab	1.62±1.07 b	6.36±5.13 ab
混合营养型Mixture	14.18±9.03 a	4.02±4.09 b	5.63±5.01 b	4.27±3.69 b

指标 Index	内生细菌 Endophytic bacteria		内生真菌 Endophytic fungi
指标 Index	r	P	r	P
可溶性糖含量	0.108	0.145	-0.047	0.628
Soluble sugar content
纤维素含量	0.210	0.023^*	0.149	0.093
Cellulose content
可溶性蛋白含量	-0.013	0.545	0.064	0.287
Soluble protein content
丙二醛含量	0.116	0.118	0.126	0.144
MDA content
超氧化物歧化酶活性	-0.102	0.846	0.051	0.221
SOD activity
过氧化物酶活性	0.176	0.028^*	0.228	0.302
POD activity
过氧化氢酶活性	0.201	0.007^**	0.030	0.318
CAT activity
根系活力Root vigor	-0.134	0.905	0.080	0.228

内生微生物 Endophytic microbial	株龄 Plant age	实证网络Empirical network								随机网络Random network
内生微生物 Endophytic microbial	株龄 Plant age	阈值 Cutoff	总节点 Total nodes	总边 Total links	幂律指数 R² of power- law	平均度 Average degree	聚类系数 Average clustering coefficient	平均距离 Average path distance	模块化 Modularity (module number)	聚类系数 Average clustering coefficient	平均距离 Average path distance	模块化 Modularity
内生细菌	ARTI	0.93	349	1466	0.740	8.401	0.345	4.779	0.561(9)	0.050±0.005^**	3.016±0.023^**	0.205±0.004^**
Endophytic	ART2	0.93	210	440	0.711	4.190	0.202	5.608	0.872(19)	0.024±0.006^**	3.774±0.047^**	0.468±0.008^**
bacteria	ART3	0.93	384	1557	0.457	8.109	0.311	5.136	0.722(9)	0.031±0.004^**	3.054±0.012^**	0.311±0.006^**
	ART4	0.93	349	1375	0.654	7.880	0.255	5.274	0.766(19)	0.037±0.004^**	3.075±0.020^**	0.311±0.005^**
内生真菌	ARTI	0.96	302	824	0.833	5.457	0.265	7.189	0.693(19)	0.036±0.006^**	3.500±0.040^**	0.394±0.006^**
Endophytic	ART2	0.96	308	603	0.806	3.916	0.234	7.732	0.832(32)	0.027±0.005^**	3.515±0.027^**	0.405±0.006^**
fungi	ART3	0.96	285	807	0.843	5.663	0.371	7.322	0.735(19)	0.043±0.007^**	3.376±0.038^**	0.380±0.006^**
	ART4	0.96	302	570	0.800	3.775	0.207	8.329	0.832(26)	0.016±0.004^**	4.156±0.046^**	0.518±0.007^**

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