Effects of overturning Lolium perenne under different nitrogen rates on carbon, nitrogen and bacterial community structure in saline soil of coastal area

doi:10.3969/j.issn.1004-1524.20231248

Acta Agriculturae Zhejiangensis ›› 2025, Vol. 37 ›› Issue (1): 159-168.DOI: 10.3969/j.issn.1004-1524.20231248

• Environmental Science • Previous Articles Next Articles

Effects of overturning Lolium perenne under different nitrogen rates on carbon, nitrogen and bacterial community structure in saline soil of coastal area

ZHU Xiaomei¹(), XING Jincheng¹^,^*(), HONG Lizhou¹, WANG Jianhong², LIU Chong¹, DONG Jing¹, SUN Guoli¹, HE Sunan¹

1. Institute of Agriculture Sciences in the Coastal Area of Jiangsu, Yancheng 224002, Jiangsu, China
2. Institute of Soil, Fertilizer and Environmental Resources, Zhejiang Academy of Agriculture Sciences, Hangzhou 310021, China

Received:2023-11-03 Online:2025-01-25 Published:2025-02-14

Abstract

Abstract:

In order to explore the effect of overturning Lolium perenne as green manure on soil carbon, nitrogen and bacterial community structure under nitrogen application rates of 0, 90, 150, 210, 270 kg·hm^-2 (based on pure N, denoted as CK, LN1, LN2, LN3, LN4, respectively), a field plot experiment was conducted in saline soil of coastal area. It was shown that the biomass of L. perenne increased with the elevated nitrogen application rates, and the highest biomass was found under the LN4 treatment, which was 64 227 kg·hm^-2. The carbon and nitrogen content of L. perenne was relatively higher under the LN2 treatment, and there was no significant difference in the C/N ratio among treatments. Compared with the control treatment without N application, the soil pH was significantly (P<0.05) decreased with nitrogen application, yet the contents of water-soluble salt, organic carbon, total nitrogen, microbial biomass carbon and microbial biomass nitrogen increased significantly. Among all the treatments, LN2 treatment exhibited the highest soil microbial quotient, which was significantly higher than the other treatments. In the soil, the relative abundance of Proteobacteria, Acidobacteria, Bacteroidetes was high. There was no significant difference in the Shannon index and Simpson index of soil bacteria among all the treatments, while the Chao1 index and Ace index of LN2 treatment was relatively higher. In general, LN2 treatment got the best comprehensive effect on reducing soil pH, increasing soil carbon and nitrogen content, and improving soil micro-ecological environment.

Key words: Lolium perenne, saline soil, soil bacterial community structure

CLC Number:

ZHU Xiaomei, XING Jincheng, HONG Lizhou, WANG Jianhong, LIU Chong, DONG Jing, SUN Guoli, HE Sunan. Effects of overturning Lolium perenne under different nitrogen rates on carbon, nitrogen and bacterial community structure in saline soil of coastal area[J]. Acta Agriculturae Zhejiangensis, 2025, 37(1): 159-168.

Figures/Tables 10

References 30

[1]	王遵亲. 中国盐渍土[M]. 北京: 科学出版社, 1993.
[2]	黄晶, 孔亚丽, 徐青山, 等. 盐渍土壤特征及改良措施研究进展[J]. 土壤, 2022, 54(1): 18-23.
	HUANG J, KONG Y L, XU Q S, et al. Progresses for characteristics and amelioration measures of saline soil[J]. Soils, 2022, 54(1): 18-23. (in Chinese with English abstract)
[3]	MATSUOKA T, ASAGI N, KOMATSUZAKI M. Response of weeds and rice yield to Italian ryegrass as a cover crop and planting density in organic farming[J]. Agronomy Journal, 2022, 114(1): 689-699.
[4]	许能祥, 董臣飞, 丁成龙, 等. 盐土和非盐土施氮对多花黑麦草增产改质效果差异的比较[J]. 草业学报, 2016, 25(11): 115-123.
	XU N X, DONG C F, DING C L, et al. Comparison of the effects of nitrogen fertilizer on the yield and feeding quality of Italian ryegrass (Lolium multiflorum) in saline and non-saline soil[J]. Acta Prataculturae Sinica, 2016, 25(11): 115-123. (in Chinese with English abstract)
[5]	张武舜, 蔡茂德, 胡浩. 苏北沿海地区牧草品种的选择及应用模式[J]. 草与畜杂志, 1988, 8(6): 13-15.
	ZHANG W S, CAI M D, HU H. Selection and application model of forage varieties in coastal areas of northern Jiangsu[J]. China Herbivore Science, 1988, 8(6): 13-15. (in Chinese)
[6]	王敬宽, 高枫舒, 张楷悦, 等. 禾本科绿肥还田对盐碱地棉田土壤碳氮及微生物量碳氮的影响[J]. 中国生态农业学报(中英文), 2023, 31(3): 396-404.
	WANG J K, GAO F S, ZHANG K Y, et al. Effects of returning gramineous green manure to cotton field on soil carbon and nitrogen in saline alkali soil[J]. Chinese Journal of Eco-Agriculture, 2023, 31(3): 396-404. (in Chinese with English abstract)
[7]	郭耀东, 程曼, 赵秀峰, 等. 轮作绿肥对盐碱地土壤性质、后作青贮玉米产量及品质的影响[J]. 中国生态农业学报, 2018, 26(6): 856-864.
	GUO Y D, CHENG M, ZHAO X F, et al. Effects of green manure rotation on soil properties and yield and quality of silage maize in saline-alkali soils[J]. Chinese Journal of Eco-Agriculture, 2018, 26(6): 856-864. (in Chinese with English abstract)
[8]	李孔晨, 卢欣石, 王铁梅. 盐胁迫下施氮水平对黑麦草氮素吸收的影响[J]. 草地学报, 2011, 19(3): 487-491.
	LI K C, LU X S, WANG T M. Effects of different nitrogen fertilizer rates on nitrogen absorption by ryegrass (Lolium muliiflorum) under salt stress[J]. Acta Agrestia Sinica, 2011, 19(3): 487-491. (in Chinese with English abstract)
[9]	沈振国, 沈其荣, 管红英, 等. NaCl胁迫下氮素营养与大麦幼苗生长和离子平衡的关系[J]. 南京农业大学学报, 1994, 17(1): 22-26.
	SHEN Z G, SHEN Q R, GUAN H Y, et al. Relationship between nitrogen nutrition and growth of barley seedlings as well as ion balance under NaCl stress[J]. Journal of Nanjing Agricultural University, 1994, 17(1): 22-26. (in Chinese)
[10]	朱小梅, 王建红, 赵宝泉, 等. 不同盐分土壤环境下绿肥腐解及养分释放动态研究[J]. 水土保持学报, 2018, 32(6): 309-314.
	ZHU X M, WANG J H, ZHAO B Q, et al. Dynamics of decomposition and nutrient release of green manure under different saline soils[J]. Journal of Soil and Water Conservation, 2018, 32(6): 309-314. (in Chinese with English abstract)
[11]	鲍士旦. 土壤农化分析[M]. 3版. 北京: 中国农业出版社, 2000.
[12]	BROOKES P C, LANDMAN A, PRUDEN G, et al. Chloroform fumigation and the release of soil nitrogen: a rapid direct extraction method to measure microbial biomass nitrogen in soil[J]. Soil Biology and Biochemistry, 1985, 17(6): 837-842.
[13]	VANCE E D, BROOKES P C, JENKINSON D S. An extraction method for measuring soil microbial biomass C[J]. Soil Biology and Biochemistry, 1987, 19(6): 703-707.
[14]	马想, 黄晶, 赵惠丽, 等. 秸秆与氮肥不同配比对红壤微生物量碳氮的影响[J]. 植物营养与肥料学报, 2018, 24(6): 1574-1580.
	MA X, HUANG J, ZHAO H L, et al. Straw and nitrogen fertilizer ratios influence microbial biomass carbon and nitrogen in red soil[J]. Journal of Plant Nutrition and Fertilizers, 2018, 24(6): 1574-1580. (in Chinese with English abstract)
[15]	唐红琴, 李忠义, 曾成城, 等. 不同绿肥种类和还田量对柑橘园土壤养分的动态影响[J]. 江苏农业科学, 2021, 49(16): 214-219.
	TANG H Q, LI Z Y, ZENG C C, et al. Dynamic impact of different green manure types and returning amounts on soil nutrients in citrus orchard[J]. Jiangsu Agricultural Sciences, 2021, 49(16): 214-219. (in Chinese with English abstract)
[16]	王娟娟, 胡珈玮, 狄霖, 等. 秸秆还田与氮肥运筹对水稻不同生育期土壤细菌群落结构的影响[J]. 江苏农业学报, 2021, 37(6): 1460-1470.
	WANG J J, HU J W, DI L, et al. Effects of straw returning and nitrogen management on soil microbial community structure at different rice growth stages[J]. Jiangsu Journal of Agricultural Sciences, 2021, 37(6): 1460-1470. (in Chinese with English abstract)
[17]	刘慧, 李子玉, 白志贵, 等. 油菜绿肥翻压还田对新疆盐碱土壤的改良效果研究[J]. 农业资源与环境学报, 2020, 37(6): 914-923.
	LIU H, LI Z Y, BAI Z G, et al. Effects of rape green manure returned to field on saline alkali soil improvement in Xinjiang[J]. Journal of Agricultural Resources and Environment, 2020, 37(6): 914-923. (in Chinese with English abstract)
[18]	黄海洋. 北方半干旱地区盐碱地上高丹草利用方式的应用研究[D]. 呼和浩特: 内蒙古农业大学, 2017.
	HUANG H Y. The study on the utilization of sorghum-sudangrass hybrid and its application semi-arid saline area in northern China[D]. Hohhot: Inner Mongolia Agricultural University, 2017. (in Chinese with English abstract)
[19]	牟云芳, 史海滨, 闫建文, 等. 秸秆和氮肥耦合管控对盐渍化土壤地力综合效应影响研究[J]. 农业环境科学学报, 2024, 43(1):1672-1680.
	MU Y F, SHI H B, YAN J W, et al. Study on the comprehensive effect of straw and nitrogen fertilizer coupling control on soil fertility in salinized soil[J]. Journal of Agro-Environment Science, 2024, 43(1): 1672-1680. (in Chinese with English abstract)
[20]	张明杰. 连续翻压黑麦草对连作植烟土壤特性和烟株生长发育的影响[D]. 郑州: 河南农业大学, 2022.
	ZHANG M J. Effects of continuous rolling ryegrass on characteristics of continuous tobacco planting soil and tobacco growth and development[D]. Zhengzhou: Henan Agricultural University, 2022. (in Chinese with English abstract)
[21]	高嵩涓, 曹卫东, 白金顺, 等. 长期冬种绿肥改变红壤稻田土壤微生物生物量特性[J]. 土壤学报, 2015, 52(4): 902-910.
	GAO S J, CAO W D, BAI J S, et al. Long-term application of winter green manures changed the soil microbial biomass properties in red paddy soil[J]. Acta Pedologica Sinica, 2015, 52(4): 902-910. (in Chinese with English abstract)
[22]	汤宏, 沈健林, 张杨珠, 等. 秸秆还田与水分管理对稻田土壤微生物量碳、氮及溶解性有机碳、氮的影响[J]. 水土保持学报, 2013, 27(1): 240-246.
	TANG H, SHEN J L, ZHANG Y Z, et al. Effect of rice straw incorporation and water management on soil microbial biomass carbon, nitrogen and dissolved organic carbon, nitrogen in a rice paddy field[J]. Journal of Soil and Water Conservation, 2013, 27(1): 240-246. (in Chinese with English abstract)
[23]	LIU L, DING M J, ZHOU L K, et al. Effects of different rice straw on soil microbial community structure[J]. Agronomy Journal, 2021, 113(2): 794-805.
[24]	解雪峰, 项琦, 吴涛, 等. 滨海湿地生态系统土壤微生物及其影响因素研究综述[J]. 生态学报, 2021, 41(1): 1-12.
	XIE X F, XIANG Q, WU T, et al. Progress and prospect of soil microorganisms and their influencing factors in coastal wetland ecosystem[J]. Acta Ecologica Sinica, 2021, 41(1): 1-12. (in Chinese with English abstract)
[25]	郑敏娜, 梁秀芝, 韩志顺, 等. 不同改良措施对盐碱土土壤细菌群落多样性的影响[J]. 草地学报, 2021, 29(6): 1200-1209.
	ZHENG M N, LIANG X Z, HAN Z S, et al. Effects of different improvement measures on the diversity of soil bacteria communities in salt-alkali soil[J]. Acta Agrestia Sinica, 2021, 29(6): 1200-1209. (in Chinese with English abstract)
[26]	TIAN W, ZHANG Z H, HU X F, et al. Short-term changes in total heavy metal concentration and bacterial community composition after replicated and heavy application of pig manure-based compost in an organic vegetable production system[J]. Biology and Fertility of Soils, 2015, 51(5): 593-603.
[27]	FIERER N, LAUBER C L, RAMIREZ K S, et al. Comparative metagenomic, phylogenetic and physiological analyses of soil microbial communities across nitrogen gradients[J]. The ISME Journal, 2012, 6(5): 1007-1017.
[28]	YAN S S, SONG J M, FAN J S, et al. Changes in soil organic carbon fractions and microbial community under rice straw return in Northeast China[J]. Global Ecology and Conservation, 2020, 22: e00962.
[29]	杨思, 杨文平, 景豆豆, 等. 麦后复种苜蓿压青还田改善土壤微生物群落结构[J]. 应用与环境生物学报, 2021, 27(4): 978-987.
	YANG S, YANG W P, JING D D, et al. Improvement of soil microbial community structure by multiple cropping alfalfa after wheat[J]. Chinese Journal of Applied and Environmental Biology, 2021, 27(4): 978-987. (in Chinese with English abstract)
[30]	SUN R B, ZHANG X X, GUO X S, et al. Bacterial diversity in soils subjected to long-term chemical fertilization can be more stably maintained with the addition of livestock manure than wheat straw[J]. Soil Biology and Biochemistry, 2015, 88: 9-18.

处理 Treatments	生物量 Biomass/(kg·hm^-2)	碳含量 Carbon content/(g·kg^-1)	氮含量 Nitrogen content/(g·kg^-1)	碳氮比 C/N ratio
CK	36 991±3 923 d	499±7 c	15.3±0.3 d	32.7±1.8 a
LN1	46 418±1 703 c	514±9 b	17.0±0.1 b	30.3±2.8 a
LN2	52 498±2 292 b	523±9 a	18.1±0.4 a	28.9±2.5 a
LN3	54 444±3 113 b	510±5 b	17.6±0.2 a	29.0±2.1 a
LN4	64 227±897 a	507±3 b	16.3±0.2 c	31.1±1.7 a

处理 Treatments	生物量 Biomass/(kg·hm^-2)	碳含量 Carbon content/(g·kg^-1)	氮含量 Nitrogen content/(g·kg^-1)	碳氮比 C/N ratio
CK	36 991±3 923 d	499±7 c	15.3±0.3 d	32.7±1.8 a
LN1	46 418±1 703 c	514±9 b	17.0±0.1 b	30.3±2.8 a
LN2	52 498±2 292 b	523±9 a	18.1±0.4 a	28.9±2.5 a
LN3	54 444±3 113 b	510±5 b	17.6±0.2 a	29.0±2.1 a
LN4	64 227±897 a	507±3 b	16.3±0.2 c	31.1±1.7 a

处理 Treatment	有机碳含量 Organic carbon content/(g·kg^-1)	全氮含量 Total nitrogen content/(g·kg^-1)	微生物生物量碳含量 Microbial biomass carbon content/(mg·kg^-1)	微生物生物量氮含量 Microbial biomass nitrogen content/(mg·kg^-1)	微生物熵 Microbial quotient/%
CK	5.74±0.07 e	0.424±0.007 d	220±5 c	30.7±0.9 c	3.80±0.11 c
LN1	6.07±0.05 d	0.493±0.008 c	239±4 b	35.7±1.2 b	3.94±0.10 b
LN2	6.30±0.04 c	0.539±0.003 b	254±9 a	38.2±0.8 ab	4.03±0.17 a
LN3	6.51±0.03 b	0.588±0.007 a	258±8 a	39.0±0.7 a	3.97±0.13 b
LN4	6.85±0.02 a	0.506±0.009 c	263±7 a	36.5±1.3 b	3.84±0.11 c

处理 Treatment	有机碳含量 Organic carbon content/(g·kg^-1)	全氮含量 Total nitrogen content/(g·kg^-1)	微生物生物量碳含量 Microbial biomass carbon content/(mg·kg^-1)	微生物生物量氮含量 Microbial biomass nitrogen content/(mg·kg^-1)	微生物熵 Microbial quotient/%
CK	5.74±0.07 e	0.424±0.007 d	220±5 c	30.7±0.9 c	3.80±0.11 c
LN1	6.07±0.05 d	0.493±0.008 c	239±4 b	35.7±1.2 b	3.94±0.10 b
LN2	6.30±0.04 c	0.539±0.003 b	254±9 a	38.2±0.8 ab	4.03±0.17 a
LN3	6.51±0.03 b	0.588±0.007 a	258±8 a	39.0±0.7 a	3.97±0.13 b
LN4	6.85±0.02 a	0.506±0.009 c	263±7 a	36.5±1.3 b	3.84±0.11 c

处理Treatment	香农指数Shannon index	辛普森指数Simpson index	Ace指数Ace index	Chao1指数Chao1 index
CK	10.1±0.1 a	0.997±0.033 a	5 881±83 d	5 965±74 c
LN1	10.5±0.4 a	0.997±0.014 a	5 985±26 c	6 027±67 b
LN2	10.4±0.2 a	0.997±0.012 a	6 583±61 a	6 530±47 a
LN3	10.4±0.4 a	0.997±0.006 a	6 413±53 b	6 463±46 a
LN4	10.1±0.3 a	0.996±0.024 a	6 043±14 c	6 025±31 b

Effects of overturning Lolium perenne under different nitrogen rates on carbon, nitrogen and bacterial community structure in saline soil of coastal area

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优势菌门 Dominant phylum	优势菌门与各土壤性状的相关系数Correlation coefficient of dominant phylum with soil proporties
优势菌门 Dominant phylum	pH	有机碳含量 Organic carbon content	全氮含量 Total nitrogen content	微生物生物量碳含量 Microbial biomass carbon content	微生物生物量氮含量 Microbial biomass nitrogen content	水溶性盐总量 Total water-soluble salt content
Pro	-0.026	0.141	0.017	0.008	-0.157	0.413
Aci	-0.275	-0.149	0.171	0.311	0.608	-0.342
Bac	-0.077	0.480	0.192	0.046	-0.252	0.523
Act	0.257	0.139	-0.126	-0.290	-0.513	0.163
Chl	-0.502	0.150	0.419	0.539	0.762	-0.107
Gem	0.667	-0.915^*	-0.729	-0.645	-0.358	-0.928^*
Fir	0.213	0.174	-0.107	-0.262	-0.540	0.408
Pat	-0.613	0.300	0.520	0.657	0.817	0.052
Nit	-0.292	-0.062	0.129	0.352	0.510	-0.109
Ver	0.939^*	-0.898^*	-0.944^*	-0.918^*	-0.811	-0.889^*
Rok	0.035	-0.339	-0.203	0.033	0.187	-0.344
Cya	-0.863	0.789	0.908^*	0.837	0.832	0.617

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[2]	WANG Hong-yan, SONG Bing-bing, NIE Ying, SUN Yan. Effect of organic farming on emissions of carbon dioxide and nitrogen oxide in alkali saline soil [J]. , 2016, 28(9): 1580-1587.
[3]	YU Haiping1， GUO Bin2，*， FU Qinglin2， DING Nengfei2， LIU Chen2， LIN Yicheng2. Coupling effects of salt stress and fertilizer application on rice yield and fertilizer using efficiency in coastal saline soil [J]. , 2016, 28(7): 1193-.
[4]	ZHENG Zhi1，2，LIU Chen2，FU Qing\\|lin2，*，GUO Bin2，LIN Yi\\|cheng2，DING Neng\\|fei2. Effects of salinity and soil moisture on microbial composition and community diversity in a coastal saline soil [J]. , 2015, 27(2): 240-.
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