Effect of ferrous sulfate on fungal community of chicken manure compost and its application potential

doi:10.3969/j.issn.1004-1524.20240085

Acta Agriculturae Zhejiangensis ›› 2025, Vol. 37 ›› Issue (1): 169-177.DOI: 10.3969/j.issn.1004-1524.20240085

• Environmental Science • Previous Articles Next Articles

Effect of ferrous sulfate on fungal community of chicken manure compost and its application potential

WANG Lutong(), JIANG Lihong, DAI Jiapeng, CHEN Liang, ZENG Guangxi, LIU Erlun, ZHOU Xiangdan, XIAO Yunhua, FANG Jun^*()

College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410125, China

Received:2024-01-25 Online:2025-01-25 Published:2025-02-14

Abstract

Abstract:

In order to examine the impact of ferrous sulfate on the fungal community during the thermophilic phase of chicken manure composting, as well as the effects of the compost products on plant growth, chicken manure and rice straw were used as raw materials, with the control group being the heap without any exogenous additives and the treatment groups being the heap with 1% or 2% ferrous sulfate addition. The changes in the composition and structure of the fungal community, as well as the growth condition of pakchoi and its nutrient quality were monitored.It was shown that adding ferrous sulfate to chicken manure composting affected the structure of the fungal community during the thermophilic phase, enhanced the diversity of the fungal community, enriched most of the dominant fungi related to lignocellulose degradation, which was conducive to the efficient degradation of difficult-to-decompose organic matter in composting, and facilitated the composting humification process. Plant height, chlorophyll content, soluble protein content and soluble sugar content were significantly (P<0.05) higher in the treatment groups compared to the control group. The effect of compost product with addition of 2% ferrous sulfate was more pronounced.

Key words: chicken manure composting, ferrous sulfate, fungal community, pakchoi

CLC Number:

X705

WANG Lutong, JIANG Lihong, DAI Jiapeng, CHEN Liang, ZENG Guangxi, LIU Erlun, ZHOU Xiangdan, XIAO Yunhua, FANG Jun. Effect of ferrous sulfate on fungal community of chicken manure compost and its application potential[J]. Acta Agriculturae Zhejiangensis, 2025, 37(1): 169-177.

Figures/Tables 7

Table 1 Basic properties of raw materials for composting

原材料 Raw material	水分含量 Water content/%	总有机碳含量 Total organic carbon content/%	全氮含量 Total nitrogen content/%	碳氮比 C/N raio
鸡粪Chicken manure	68.53±2.75	57.06±16.04	4.99±0.61	11.31±2.25
稻草Straw	11.37±0.21	55.51±4.24	1.03±0.08	54.11±7.78

Fig.1 Changes of temperature during the composting (A) and the germination index (GI) (B) after composting under different treatmetns GI, Germination index. Bars marked without the same letters indicate significant difference at P<0.05. The same as below.

Fig.2 Fungal community composition at the phylum level (A), genus level (B), and structural analysis based on principal co-ordinates analysis (PCoA) (C) CK1, CK2 and CK3 were 3 parallel treatment groups of CK; G21, G22 and G23 were 3 parallel treatment groups of G21; G31, G32 and G33 were 3 parallel treatment groups of FS2.

Table 2 The alpha diversity indexes of the fungal community

处理 Treatment	丰富度指数 Richness	Shannon指数 Shaannon index	Simpson指数 Simpson index	Chao1指数 Chao1 index	ACE指数 ACE index
CK	178.00±34.83	3.77±1.03	0.90±0.12	180.33±33.61	182.07±33.39
FS1	172.00±16.56	4.21±0.35	0.96±0.03	175.09±15.79	179.31±14.10
FS2	975.00±1 233.38	5.22±0.74	0.99±0.01	978.98±1 233.88	989.43±1 244.98

Fig.3 Distribution of significant differences between LEfSe groups (A) and distribution of LDA values (B) p_, c_, o_, f_, g_ and s_ denote phylum, class, order, family, genus and species level, respectively.

Table 3 Effect of different organic fertilizers on growth indexes of pakchoi

处理 Treatment	株高 Plant height/cm	根长 Root length/cm	干重 Dry weight/g	SPAD	根系活力 Root activity/(μg·g^-1·h^-1)
TCK	3.35±0.07 c	7.65±0.07 a	0.07±0.01 b	4.80±0.00 d	255.18±11.60 c
VCK	6.65±0.07 b	4.85±0.07 a	0.10±0.01 ab	5.90±0.00 b	366.64±17.81 b
VFS1	8.20±0.50 a	9.15±0.92 a	0.07±0.01 b	5.55±0.07 c	259.24±35.40 c
VFS2	8.70±0.28 a	8.90±1.91 a	0.13±0.01 a	6.50±0.10 a	755.16±20.94 a

Fig.4 Effects of different treatments on contents of vitamin C, soluble sugar and soluble protein

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Effect of ferrous sulfate on fungal community of chicken manure compost and its application potential

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