Effects of γ-polyglutamic acid fermentation broth on growth of pakchoi and utilization rate of nitrogen and phosphorus fertilizer

doi:10.3969/j.issn.1004-1524.2023.02.10

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (2): 329-337.DOI: 10.3969/j.issn.1004-1524.2023.02.10

• Horticultural Science • Previous Articles Next Articles

Effects of γ-polyglutamic acid fermentation broth on growth of pakchoi and utilization rate of nitrogen and phosphorus fertilizer

HE Yu¹^,²(), LYU Weiguang²^,³^,⁴^,⁵, LI Shuangxi²^,³^,⁴^,⁵, ZHENG Xianqing²^,³^,⁴^,⁵, ZHANG Hanlin²^,³^,⁴, ZHANG Juanqin²^,³, ZHANG Haiyun²^,⁴, BAI Naling²^,³^,⁴^,⁵^,^*(), LIU Shanliang⁶

1. Faculty of Marine Ecology and Environment, Shanghai Ocean University, Shanghai 201306, China
2. Eco-Environmental Protection Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China
3. Shanghai Agri-Environmental and Cultivated Land Conservation of Scientific Observation and Experiment Station, Ministry of Agriculture and Rural Affairs, Shanghai 201403, China
4. Shanghai Agri-Environmental Protection Monitoring Station, Shanghai 201403, China
5. Shanghai Key Laboratory of Protected Horticulture Technology, Shanghai 201403, China
6. SEEK Bio-Technology (Shanghai) Co., Ltd., Shanghai 201108, China

Received:2021-09-19 Online:2023-02-25 Published:2023-03-14
Contact: BAI Naling

Abstract

Abstract:

In order to study the effects of γ-polyglutamic acid (γ-PGA) and its fermentation broth as microbial fertilizer on pakchoi growth and soil nutrients, so as to provide basis for chemical fertilizer reduction and sustainable agricultural development, Brassica rapa L.ssp.chinensis L. was taken as the research object, the pot experiment was carried out by using the γ-PGA producing bacterium A-5 and its fermentation product. Then, the effects of γ-PGA and γ-PGA fermentation broth on the quality of pakchoi, soil physicochemical properties and soil enzyme activities were measured, and the N/P fertilizer use efficiencies and economic benefits were calculated. The pot experiment proved that the γ-PGA and γ-PGA fermentation broth produced by A-5 had better growth-promoting and synergistic effects. Compared with the treatment of nitrogen fertilizer reduced by 30% (-N), pure γ-PGA (-N+PGA) and γ-PGA fermentation broth (-N+FJY) increased vegetable yield by 20.19% and 37.63% respectively, and the -N+FJY treatment had better growth promoting effect. Under the treatment of γ-PGA fermentation broth, containing a large amount of B.subtilis sp. A-5, the fertilizer utilization rate of N/P were significantly higher than other treatments, which had higher economic benefits than γ-PGA. In summary, γ-PGA acid fermentation produced by B.subtilis sp. A-5 has the significant effects on promoting vegetable growth and improving fertilizer utilization ratio, which can provide a basis for its application in agriculture.

Key words: γ-polyglutamic acid, pakchoi, Bacillus subtilis, fermentation, fertilizer utilization rate, economic benefit

CLC Number:

S634

HE Yu, LYU Weiguang, LI Shuangxi, ZHENG Xianqing, ZHANG Hanlin, ZHANG Juanqin, ZHANG Haiyun, BAI Naling, LIU Shanliang. Effects of γ-polyglutamic acid fermentation broth on growth of pakchoi and utilization rate of nitrogen and phosphorus fertilizer[J]. Acta Agriculturae Zhejiangensis, 2023, 35(2): 329-337.

Figures/Tables 6

References 33

[1]	FAO. FAOSTA T Data[DB/OL].http://www.fao.org/faostat/en/, 2020/2022-03-03.
[2]	ROXANA-GABRIELA P O P A. Soil pollution with chemical fertilizers[J]. Fiabilitate Si Durabilitate, 2018, 1(21):369-374.
[3]	BAI Y C, CHANG Y Y, HUSSAIN M, et al. Soil chemical and microbiological properties are changed by long-term chemical fertilizers that limit ecosystem functioning[J]. Microorganisms, 2020, 8(5):694.
[4]	JIN S Q, ZHOU F. Zero growth of chemical fertilizer and pesticide use: China’s objectives, progress and challenges[J]. Journal of Resources and Ecology, 2018, 9(sp1): 50-58.
[5]	STAMENKOVIĆ S, BEŠKOSKI V, KARABEGOVIĆ I, et al. Microbial fertilizers: a comprehensive review of current findings and future perspectives[J]. Spanish Journal of Agricultural Research, 2018, 16(1): e09R01.
[6]	MEYNELL G G, MEYNELL E. The biosynthesis of poly d-glutamic acid, the capsular material of Bacillus anthracis[J]. Journal of General Microbiology, 1966, 43(1): 119-138.
[7]	PRADEEPKUMAR P, SANGEETHA R, GUNASEELAN S, et al. Folic acid conjugated polyglutamic acid drug vehicle synthesis through deep eutectic solvent for targeted release of paclitaxel[J]. ChemistrySelect, 2019, 4(35): 10225-10235.
[8]	BAJAJ I B, SINGHAL R S. Flocculation properties of poly( γ-glutamic acid) produced from Bacillus subtilis isolate[J]. Food and Bioprocess Technology, 2011, 4(5): 745-752.
[9]	张文, 张树清, 王学江. γ-聚谷氨酸的微生物合成及其在农业生产中的应用[J]. 中国农学通报, 2014, 30(6): 40-45.
	ZHANG W, ZHANG S Q, WANG X J. The microbial synthesis of γ-polyglutamic acid and its application in agricultural production[J]. Chinese Agricultural Science Bulletin, 2014, 30(6): 40-45. (in Chinese with English abstract)
[10]	王进. 产聚谷氨酸菌种的筛选以及其在肥料增效剂方面的应用研究[D]. 武汉: 武汉工程大学, 2018.
	WANG J. Screening of strains producing γ-poly glutamic acid and research on fertilizer efficiency[D]. Wuhan: Wuhan Institute of Technology, 2018. (in Chinese with English abstract)
[11]	BAI N L, ZHANG H L, LI S X, et al. Effects of application rates of poly-γ-glutamic acid on vegetable growth and soil bacterial community structure[J]. Applied Soil Ecology, 2020, 147: 103405.
[12]	高俊凤. 植物生理学实验指导[M]. 北京: 高等教育出版社, 2006.
[13]	张水华. 食品分析实验[M]. 北京: 化学工业出版社, 2006.
[14]	鲍士旦. 土壤农化分析[M]. 3版. 北京: 中国农业出版社, 2000.
[15]	鲁如坤. 土壤农业化学分析方法[M]. 北京: 中国农业科技出版社, 2000.
[16]	王阳, 赵兰坡, 朱孟龙, 等. 长期单施化肥对土壤有机质的影响[J]. 吉林农业科学, 2015, 40(1): 45-50.
	WANG Y, ZHAO L P, ZHU M L, et al. Effects of long-term application of chemical fertilizer on soil organic matter[J]. Journal of Jilin Agricultural Sciences, 2015, 40(1): 45-50. (in Chinese with English abstract)
[17]	张智, 乔艳, 刘东海, 等. 氮肥减施对油菜产量、氮素吸收与利用的影响[J]. 中国土壤与肥料, 2020(2): 140-145, 183.
	ZHANG Z, QIAO Y, LIU D H, et al. Effects of reducing nitrogen application on yield, nitrogen absorption and utilization of oilseed rape[J]. Soil and Fertilizer Sciences in China, 2020(2): 140-145, 183. (in Chinese with English abstract)
[18]	王亚玲. 单控和组配施肥模式对设施黄瓜养分利用、品质及土壤性状的影响[D]. 保定: 河北农业大学, 2020.
	WANG Y L. Effects of single control and combined fertilization on nutrient utilization, quality and soil properties of greenhouse cucumber[D]. Baoding: Hebei Agricultural University, 2020. (in Chinese with English abstract)
[19]	ZHANG L, YANG X, GAO D, et al. Effects of poly-γ-glutamic acid(γ-PGA)on plant growth and its distribution in a controlled plant-soil system[J]. Scientific Reports, 2017, 7: 6090.
[20]	黄天悦, 高鹏, 王进, 等. γ-聚谷氨酸的发酵优化及其对辣椒生长的影响[J]. 武汉工程大学学报, 2020, 42(2): 143-152.
	HUANG T Y, GAO P, WANG J, et al. Fermentation optimization of gamma-polyglutamic acid and its effect on growth of pepper[J]. Journal of Wuhan Institute of Technology, 2020, 42(2): 143-152. (in Chinese with English abstract)
[21]	贾艳萍, 殷爱鸣, 孙艳梅, 等. 产聚谷氨酸菌株的筛选及菌株发酵液对玉米幼苗抗旱性的作用[J]. 生物技术通报, 2017, 33(10): 135-142.
	JIA Y P, YIN A M, SUN Y M, et al. Screen of poly-γ-gamma glutamic acid producing bacteria and their glutamic acid fermentation broth’s function on the drought resistance of maize[J]. Biotechnology Bulletin, 2017, 33(10): 135-142. (in Chinese with English abstract)
[22]	乔长晟, 王凡, 李雪, 等. 含聚谷氨酸高产菌的复合微生物肥料及其制备方法和应用: CN103204732B[P]. 2015-02-25.
[23]	蔡树美, 诸海焘, 朱恩, 等. 上海地区典型设施菜地的生菜适宜施氮量研究[J]. 中国土壤与肥料, 2014(2): 49-52, 71.
	CAI S M, ZHU H T, ZHU E, et al. The optimum N fertilization rate of lettuce of typical plastic greenhouse in Shanghai[J]. Soil and Fertilizer Sciences in China, 2014(2): 49-52, 71. (in Chinese with English abstract)
[24]	蒯佳琳, 马彦霞, 侯栋, 等. 稳定性肥料配施微生物菌剂对莴笋生长及品质的影响研究[J]. 干旱地区农业研究, 2021, 39(2): 24-30.
	KUAI J L, MA Y X, HOU D, et al. Study on the effects of stabilized fertilizer combined with microbial agent on growth and quality of lettuce[J]. Agricultural Research in the Arid Areas, 2021, 39(2): 24-30. (in Chinese with English abstract)
[25]	王振. 复合微生物菌剂对水稻生长发育影响研究[D]. 沈阳: 沈阳农业大学, 2017: 1-71.
	WANG Z. Effects of compound microbial agents on the growth and development of rice[D]. Shenyang: Shenyang Agricultural University, 2017: 1-71. (in Chinese with English abstract)
[26]	王文静, 魏静, 马文奇, 等. 氮肥用量和秸秆根茬碳投入对黄淮海平原典型农田土壤有机质积累的影响[J]. 生态学报, 2010, 30(13): 3591-3598.
	WANG W J, WEI J, MA W Q, et al. Effect of nitrogen amendment and straw-stubble input on accumulation of soil organic matter in typical farmlands of Huang-Huai-Hai Plain[J]. Acta Ecologica Sinica, 2010, 30(13): 3591-3598. (in Chinese with English abstract)
[27]	刘学彤, 郑春莲, 曹薇, 等. 长期定位施肥对土壤有机质、不同形态氮含量及作物产量的影响[J]. 作物杂志, 2021(4): 130-135.
	LIU X T, ZHENG C L, CAO W, et al. Effects of long-term located fertilization on soil organic matter, nitrogen forms and crop yields[J]. Crops, 2021(4): 130-135. (in Chinese with English abstract)
[28]	褚义红. 不同微生物菌肥对温室生菜生长、品质、产量及氮素积累的影响[D]. 呼和浩特: 内蒙古农业大学, 2014: 1-53.
	CHU Y H. Effects of different microbial fertilizers on the growth, the quality, the production and the nitrogen accumulation of[D]. Hohhot: Inner Mongolia Agricultural University, 2014: 1-53. (in Chinese with English abstract)
[29]	王超, 李刚, 黄思杰, 等. 枯草芽胞杆菌菌肥对有机冬瓜根区土壤微生态的影响[J]. 微生物学通报, 2019, 46(3): 563-576.
	WANG C, LI G, HUANG S J, et al. Effect of Bacillus subtilis microbial fertilizer on root-zone soil microbial ecology of organic Chinese watermelon[J]. Microbiology China, 2019, 46(3): 563-576. (in Chinese with English abstract)
[30]	XU Z, WAN C, XU X, et al. Effect of poly(γ-glutamic acid) on wheat productivity, nitrogen use efficiency and soil microbes[J]. Journal of Soil Science and Plant Nutrition, 2013, 13(3): 744-755.
[31]	田蜜蜜. 过氧化氢酶在拟南芥生长发育中的功能及作用机制分析[D]. 合肥: 合肥工业大学, 2021.
	TIAN M M. Analysis of the function and mechanism of catalase in the growth and development in Arabidopsis thaniala[D]. Hefei: Hefei University of Technology, 2021. (in Chinese with English abstract)
[32]	刘乐, 费良军, 陈琳, 等. γ-聚谷氨酸对土壤结构、养分平衡及菠菜产量的影响[J]. 水土保持学报, 2019, 33(1): 277-282, 287.
	LIU L, FEI L J, CHEN L, et al. Effects of γ-polyglutamic acid on soil structure, nutrient balance and spinach yield[J]. Journal of Soil and Water Conservation, 2019, 33(1): 277-282, 287. (in Chinese with English abstract)
[33]	吴珏, 王伟群, 瞿洁, 等. 化肥减量配施微生物肥料对青菜生长的影响[J]. 上海蔬菜, 2020(2): 52-54.
	WU J, WANG W Q, QU J, et al. Effects of chemical fertilizer reduction combined with microbial fertilizer on the growth of vegetables[J]. Shanghai Vegetables, 2020(2): 52-54. (in Chinese)

处理 Treatment	尿素 Urea/ (kg·hm^-2)	过磷酸钙 Super- phosphate/ (kg·hm^-2)	氯化钾 Potassium chloride/ (kg·hm^-2)
CK	0	0	0
CF	285.96	350.88	219.30
-N	200.00	350.88	219.30
-N+PGA	200.00	350.88	219.30
-N+FJY	200.00	350.88	219.30

处理 Treatment	尿素 Urea/ (kg·hm^-2)	过磷酸钙 Super- phosphate/ (kg·hm^-2)	氯化钾 Potassium chloride/ (kg·hm^-2)
CK	0	0	0
CF	285.96	350.88	219.30
-N	200.00	350.88	219.30
-N+PGA	200.00	350.88	219.30
-N+FJY	200.00	350.88	219.30

处理 Treatment	产量 Yield/(kg· hm^-2)	叶绿素相对含量 SPAD	叶面积 Leaf area/ cm²	V_C/ (μg·g^-1)	硝态氮 NO₃-N/ (mg·kg^-1)	总氮 P-TN/ (g·kg^-1)	总磷 P-TP/ (g·kg^-1)
CK	16 106.73±33.08 d	51.74±2.42 b	40.87±1.62 d	342.53±46.53 a	50.11±11.08 c	9.50±1.30 b	0.81±0.24 c
CF	22 923.10±50.30 b	59.46±2.65 a	53.30±1.94 c	330.35±29.78 a	439.03±20.76 a	21.28±0.81 a	2.08±0.25 b
-N	19 171.35±127.85 c	61.80±3.05 a	52.40±2.30 c	367.76±27.46 a	69.59±14.92 c	19.87±0.74 a	2.22±0.26 b
-N+PGA	23 042.98±149.80 b	59.53±3.25 a	64.45±2.85 b	355.27±27.04 a	131.67±5.62 b	21.99±1.28 a	2.47±0.29 ab
-N+FJY	26 385.09±92.02 a	62.15±2.72 a	94.22±2.90 a	376.77±6.17 a	95.65±13.35 bc	22.35±0.91 a	2.92±0.22 a

处理 Treatment	产量 Yield/(kg· hm^-2)	叶绿素相对含量 SPAD	叶面积 Leaf area/ cm²	V_C/ (μg·g^-1)	硝态氮 NO₃-N/ (mg·kg^-1)	总氮 P-TN/ (g·kg^-1)	总磷 P-TP/ (g·kg^-1)
CK	16 106.73±33.08 d	51.74±2.42 b	40.87±1.62 d	342.53±46.53 a	50.11±11.08 c	9.50±1.30 b	0.81±0.24 c
CF	22 923.10±50.30 b	59.46±2.65 a	53.30±1.94 c	330.35±29.78 a	439.03±20.76 a	21.28±0.81 a	2.08±0.25 b
-N	19 171.35±127.85 c	61.80±3.05 a	52.40±2.30 c	367.76±27.46 a	69.59±14.92 c	19.87±0.74 a	2.22±0.26 b
-N+PGA	23 042.98±149.80 b	59.53±3.25 a	64.45±2.85 b	355.27±27.04 a	131.67±5.62 b	21.99±1.28 a	2.47±0.29 ab
-N+FJY	26 385.09±92.02 a	62.15±2.72 a	94.22±2.90 a	376.77±6.17 a	95.65±13.35 bc	22.35±0.91 a	2.92±0.22 a

处理 Treatment	酸碱度 pH	有机质含量 SOM/%	蔗糖酶活性 S-SC/(mg·d^-1·g^-1)	脲酶活性 S-UE/(μg·d^-1·g^-1)	过氧化氢酶活性 S-CAT/(μmol·d^-1·g^-1)
CK	8.08±0.02 a	1.69±0.06 b	41.02±5.57 b	458.37±74.53 d	45.29±3.11 a
CF	8.11±0.04 a	1.59±0.12 b	50.83±7.56 b	787.26±84.89 c	46.54±1.56 a
-N	8.08±0.05 a	2.17±0.16 a	58.16±4.73 ab	843.55±45.57 bc	45.49±1.63 a
-N+PGA	8.04±0.04 b	2.04±0.27 a	68.29±7.53 a	941.80±43.89 b	45.96±2.14 a
-N+FJY	8.04±0.03 a	1.51±0.03 b	64.63±5.04 a	1087.43±81.54 a	47.65±1.87 a

Effects of γ-polyglutamic acid fermentation broth on growth of pakchoi and utilization rate of nitrogen and phosphorus fertilizer

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处理 Treatment	氮肥N fertilizer			磷肥P fertilizer
处理 Treatment	偏生产力 PFPN/(g·g^-1)	农学效率 AEN/(g·g^-1)	当季利用率 NUE/%	偏生产力 PFPP/(g·g^-1)	农学效率 AEP/(g·g^-1)	当季利用率 PUE/%
CF	174.21±3.82 d	51.80±3.82 c	28.00±2.03 c	261.32±5.73 b	77.70±5.73 b	4.40±0.63 b
-N	208.15±13.88 c	33.27±13.88 c	28.48±4.67 c	218.55±14.57 c	34.93±14.57 c	3.90±0.53 b
-N+PGA	250.18±16.27 b	75.31±16.27 b	42.55±3.21 b	262.69±17.08 b	79.07±17.08 b	5.22±1.56 b
-N+FJY	286.47±9.99 a	111.59±9.99 a	52.15±2.90 a	300.79±10.49 a	117.17±10.49 a	8.03±0.73 a

处理 Treatment	产值/(万元·hm^-2) Output value/ (10⁴ yuan·hm^-2)	肥料成本/(万元·hm^-2) Fertilizer cost/ (10⁴ yuan·hm^-2)	菌剂成本/(万元·hm^-2) Microbial fertilizer cost/ (10⁴ yuan·hm^-2)	净产值/(万元·hm^-2) Net output value/ (10⁴ yuan·hm^-2)	较CK增收/(万元·hm^-2) Value-added than CK/ (10⁴ yuan·hm^-2)
CK	9.66	0	0	9.66	—
CF	13.75	0.13	0	13.62	3.96
-N	11.50	0.10	0	11.40	1.74
-N+PGA	13.83	0.10	1.58	12.15	2.49
-N+FJY	15.83	0.10	1.26	14.47	4.81

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