Effects of sulfamonomethoxine on bacterial community structure in feces of laying hens

doi:10.3969/j.issn.1004-1524.2022.02.09

Acta Agriculturae Zhejiangensis ›› 2022, Vol. 34 ›› Issue (2): 284-292.DOI: 10.3969/j.issn.1004-1524.2022.02.09

• Animal Science • Previous Articles Next Articles

Effects of sulfamonomethoxine on bacterial community structure in feces of laying hens

WANG Jingge¹^,²^,³(), JI Xiaofeng²^,³, WU Jing²^,³, YANG Hua²^,³, TANG Biao²^,³^,^*(), DING Bao'an¹^,^*()

1. College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China
2. Institute of Agro-Product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
3. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2021-03-15 Online:2022-02-25 Published:2022-03-02
Contact: TANG Biao,DING Bao'an

Abstract

Abstract:

In order to explore the effects of sulfamonomethoxine (SMM) addition into the drinking water during breeding on the structure of fecal bacterial community of laying hens, a total of 260 Hailan brown laying hens were randomly divided into two groups (group A1: no SMM was added into drinking water; group B1: SMM was added into drinking water), and fresh feces were collected on the 5th day of feeding. Then, no SMM was added in group B1, and this group was renamed as group B2, and group A1 was renamed as A2 (as the control of group B2), and fresh feces were collected again after 7 days of feeding. The total DNA was extracted and sequenced by Illumina MiSeq platform. Based on the variable region of V3-V4 of 16S rDNA, diversity and fecal microbial community structure were analyzed. The addition of SMM reduced the bacterial diversity and richness of the laying hens, but the abundance of Lactobacillus and Acinetobacter were significantly (P<0.05) increased. One week after stopping SMM addtiion, the bacterial diversity and richness of the laying hens recovered, and the abundance of Acinetobacter was significantly (P<0.05) decreased. It was concluded that feeding SMM could change the structure of the intestinal community of laying hens, yet the intestinal community could recover after discontinuation of SMM feeding. The results increased insight into the harm of antibiotics and provided references for the standard use of antibiotics.

Key words: sulfamonomethoxine, laying hen, bacterial community structure

CLC Number:

S811.6

WANG Jingge, JI Xiaofeng, WU Jing, YANG Hua, TANG Biao, DING Bao'an. Effects of sulfamonomethoxine on bacterial community structure in feces of laying hens[J]. Acta Agriculturae Zhejiangensis, 2022, 34(2): 284-292.

Figures/Tables 6

Fig.1 Venn diagram of sample OTUs

Table 1 Diversity statistics

样品 Sample	Coverage指数 Coverage index	Ace指数 Ace index	Simpson指数 Simpson index	Sobs指数 Sobs index
A1	0.996	683.674	0.092	569.727
B1	0.997	610.480	0.133	442.667
A2	0.995	746.428	0.080	615.636
B2	0.995	745.682	0.113	589.533

Fig.2 Circos diagram of bacterial communities composition of different samples at phylum level

Fig.3 Heat map of bacterial communities composition of different samples at genus level

Fig.4 Differences within groups at genus level

Fig.5 Differences within groups at genus level between group A1 and B1, group B1 and B2 The value corresponding to the dot represents the difference in the average relative abundance of species in the two groups. The color of the dot shows the grouping color with a relatively larger species abundance, and the type I interval on the dot is the upper and lower limit of the difference. The P value is on the right. *, 0.01<P≤0.05; **, 0.001<P≤0.01; ***, P≤0.001.

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Effects of sulfamonomethoxine on bacterial community structure in feces of laying hens

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