Effects of different soil disinfection methods on soil fungal diversity and community structure

doi:10.3969/j.issn.1004-1524.2023.03.17

Acta Agriculturae Zhejiangensis ›› 2023, Vol. 35 ›› Issue (3): 639-646.DOI: 10.3969/j.issn.1004-1524.2023.03.17

• Environmental Science • Previous Articles Next Articles

Effects of different soil disinfection methods on soil fungal diversity and community structure

ZHU Shijun¹(), WANG Lili¹, JIN Shuquan¹^,^*(), ZHOU Jinbo¹, WANG Feng¹, LU Xiaohong²

1. Ningbo Academy of Agricultural Science, Ningbo 315101, Zhejiang, China
2. Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received:2022-01-29 Online:2023-03-25 Published:2023-04-07

Abstract

Abstract:

In the present study, strawberry greenhouse with severe continuous cropping obstacles was used as the research object, the effects of four soil disinfection methods of high temperature control (T1), quicklime disinfection (T2), calcium cyanamide disinfection (T3), and dazomet disinfection (T4) on soil fungal diversity and soil fungal community structure were compared. It was shown that the soil fungal diversity under different soil disinfection methods were reduced to varying degrees as compared with the control (CK), and various alpha diversity indexes decreased as T4>T3>T2>T1. For soil dominant fungi at genus level (relative abundance>1%), the relative abundance of Fusarium, Thielavia, Mortierella, Verticillium and Acremonium were decreased under four soil disinfection treatments compared with CK, while the relative abundance of Chaetomium and Penicillium were increased. According to the impact of soil disinfection methods on soil fungal diversity and soil fungal community structure, the performance of dazomet disinfection was the best and was followed by calcium cyanamide disinfection. Given the economic cost, calcium cyanamide disinfection was a preferable choice.

Key words: continuous cropping obstacles, soil disinfection, fungal diversity, community structure

CLC Number:

S154.3
S158

ZHU Shijun, WANG Lili, JIN Shuquan, ZHOU Jinbo, WANG Feng, LU Xiaohong. Effects of different soil disinfection methods on soil fungal diversity and community structure[J]. Acta Agriculturae Zhejiangensis, 2023, 35(3): 639-646.

Figures/Tables 5

References 28

[1]	郭世荣, 孙锦, 束胜, 等. 我国设施园艺概况及发展趋势[J]. 中国蔬菜, 2012(18): 1-14.
	GUO S R, SUN J, SHU S, et al. Analysis of general situation, characteristics, existing problems and development trend of protected horticulture in China[J]. China Vegetables, 2012(18): 1-14. (in Chinese with English abstract)
[2]	王芳. 茄子连作障碍机理研究[D]. 北京: 中国农业大学, 2003: 9-11.
	WANG F. The mechanism of eggplant (Solanum melongena L.) replanting problem[D]. Beijing: China Agricultural University, 2003: 9-11. (in Chinese with English abstract)
[3]	FANG W S, WANG X L, HUANG B, et al. Comparative analysis of the effects of five soil fumigants on the abundance of denitrifying microbes and changes in bacterial community composition[J]. Ecotoxicology and Environmental Safety, 2020, 187: 109850. DOI URL
[4]	LI K, DILEGGE M J, MINAS I S, et al. Soil sterilization leads to re-colonization of a healthier rhizosphere microbiome[J]. Rhizosphere, 2019, 12: 100176. DOI URL
[5]	张大琪, 颜冬冬, 方文生, 等. 生物熏蒸: 环境友好型土壤熏蒸技术[J]. 农药学学报, 2020, 22(1): 11-18.
	ZHANG D Q, YAN D D, FANG W S, et al. Biofumigation: an environment-friendly soil fumigation technology[J]. Chinese Journal of Pesticide Science, 2020, 22(1): 11-18. (in Chinese with English abstract)
[6]	曹坳程, 郭美霞, 王秋霞, 等. 世界土壤消毒技术进展[J]. 中国蔬菜, 2010(21): 17-22.
	CAO A C, GUO M X, WANG Q X, et al. Progress of soil disinfection technology in the world[J]. China Vegetables, 2010(21): 17-22. (in Chinese)
[7]	SUZUKI K, KASHIWA N, NOMURA K, et al. Impacts of application of calcium cyanamide and the consequent increase in soil pH on N₂O emissions and soil bacterial community compositions[J]. Biology and Fertility of Soils, 2021, 57(2): 269-279. DOI
[8]	MAO L G, JIANG H Y, WANG Q X, et al. Efficacy of soil fumigation with dazomet for controlling ginger bacterial wilt (Ralstonia solanacearum) in China[J]. Crop Protection, 2017, 100: 111-116. DOI URL
[9]	PERVAIZ Z H, IQBAL J, ZHANG Q M, et al. Continuous cropping alters multiple biotic and abiotic indicators of soil health[J]. Soil Systems, 2020, 4(4): 59. DOI URL
[10]	DONG L L, XU J, ZHANG L J, et al. High-throughput sequencing technology reveals that continuous cropping of American ginseng results in changes in the microbial community in arable soil[J]. Chinese Medicine, 2017, 12: 18. DOI PMID
[11]	张子龙, 王文全. 植物连作障碍的形成机制及其调控技术研究进展[J]. 生物学杂志, 2010, 27(5): 69-72.
	ZHANG Z L, WANG W Q. Progress on formation mechanism and control measurements of continuous cropping obstacles in plants[J]. Journal of Biology, 2010, 27(5): 69-72. (in Chinese with English abstract)
[12]	MESZKA B, MALUSÀ E. Effects of soil disinfection on health status, growth and yield of strawberry stock plants[J]. Crop Protection, 2014, 63: 113-119. DOI URL
[13]	MA L J, GEISER D M, PROCTOR R H, et al. Fusarium pathogenomics[J]. Annual Review of Microbiology, 2013, 67: 399-416. DOI URL
[14]	ZHAO J, MEI Z, ZHANG X, et al. Suppression of Fusarium wilt of cucumber by ammonia gas fumigation via reduction of Fusarium population in the field[J]. Scientific Reports, 2017, 7: 43103. DOI
[15]	SUTTON J C. Epidemiology of wheat head blight and maize ear rot caused by Fusarium graminearum[J]. Canadian Journal of Plant Pathology, 1982, 4(2): 195-209. DOI URL
[16]	ZHU J H, REN Z J, HUANG B, et al. Effects of fumigation with allyl isothiocyanate on soil microbial diversity and community structure of tomato[J]. Journal of Agricultural and Food Chemistry, 2020, 68(5): 1226-1236. DOI PMID
[17]	NICOLA L, TURCO E, ALBANESE D, et al. Fumigation with dazomet modifies soil microbiota in apple orchards affected by replant disease[J]. Applied Soil Ecology, 2017, 113: 71-79. DOI URL
[18]	LI F, CHEN L, REDMILE-GORDON M, et al. Mortierella elongata’s roles in organic agriculture and crop growth promotion in a mineral soil[J]. Land Degradation & Development, 2018, 29(6): 1642-1651. DOI URL
[19]	UEHLING J, GRYGANSKYI A, HAMEED K, et al. Comparative genomics of Mortierella elongata and its bacterial endosymbiont Mycoavidus cysteinexigens[J]. Environmental Microbiology, 2017, 19(8): 2964-2983. DOI URL
[20]	IBRAHIM S R M, ALTYAR A E, MOHAMED S G A, et al. Genus Thielavia: phytochemicals, industrial importance and biological relevance[J]. Natural Product Research, 2022, 36(19):5108-5123. DOI URL
[21]	MTIBAÀ R, EZZANAD A, ARANDA E, et al. Biodegradation and toxicity reduction of nonylphenol, 4-tert-octylphenol and 2,4-dichlorophenol by the ascomycetous fungus Thielavia sp HJ22: identification of fungal metabolites and proposal of a putative pathway[J]. Science of the Total Environment, 2020, 708: 135129. DOI URL
[22]	DANGI S R, GERIK J S, TIRADO-CORBALÁ R, et al. Soil microbial community structure and target organisms under different fumigation treatments[J]. Applied and Environmental Soil Science, 2015, 2015: 673264.
[23]	NIELSEN J C, GRIJSEELS S, PRIGENT S, et al. Global analysis of biosynthetic gene clusters reveals vast potential of secondary metabolite production in Penicillium species[J]. Nature Microbiology, 2017, 2: 17044. DOI URL
[24]	SOYTONG K, KANOKMEDHAKUL S, KUKONGVIRIYAPA V, et al. Application of Chaetomium species (Ketomium) as a new broad spectrum biological fungicide for plant disease control: a review article[J]. Fungi Diversity, 2001, 7: 1-15.
[25]	DUPONNOIS R, KISA M, PLENCHETTE C. Phosphate-solubilizing potential of the nematophagous fungus Arthrobotrys oligospora[J]. Journal of Plant Nutrition and Soil Science, 2006, 169(2): 280-282. DOI URL
[26]	NIU X M, ZHANG K Q. Arthrobotrys oligospora: a model organism for understanding the interaction between fungi and Nematodes[J]. Mycology, 2011, 2(2): 59-78. DOI URL
[27]	LEANDRO L F S, GUZMAN T, FERGUSON L M, et al. Population dynamics of Trichoderma in fumigated and compost-amended soil and on strawberry roots[J]. Applied Soil Ecology, 2007, 35(1): 237-246. DOI URL
[28]	XUE C, SHEN Z, HAO Y, et al. Fumigation coupled with bio-organic fertilizer for the suppression of watermelon Fusarium wilt disease re-shapes the soil microbiome[J]. Applied Soil Ecology, 2019, 140: 49-56. DOI URL

处理 Treatment	OTUs	Chao1指数 Chao1 index	物种数 Observed species	香农指数 Shannon index	辛普森指数 Simpson index
CK	465	546.98	455.06	5.24	0.94
T1	446	521.58	439.27	5.17	0.93
T2	425	509.33	423.53	5.10	0.93
T3	391	465.71	388.98	4.82	0.91
T4	370	432.06	369.03	4.77	0.90

处理 Treatment	OTUs	Chao1指数 Chao1 index	物种数 Observed species	香农指数 Shannon index	辛普森指数 Simpson index
CK	465	546.98	455.06	5.24	0.94
T1	446	521.58	439.27	5.17	0.93
T2	425	509.33	423.53	5.10	0.93
T3	391	465.71	388.98	4.82	0.91
T4	370	432.06	369.03	4.77	0.90

菌门Phylum	菌属Species	CK	T1	T2	T3	T4
子囊菌门Ascomycota	镰刀菌属Fusarium	5.77	2.74	1.88	1.05	0.47
	梭孢壳属Thielavia	5.14	3.95	3.71	4.90	1.86
	棒囊壳属Corynascus	3.28	2.32	2.91	7.73	2.55
	轮枝菌属Verticillium	2.45	1.29	0.99	0.88	0.40
	脉孢菌属Neurospora	1.63	1.66	5.66	2.69	1.19
	枝顶孢霉属Acremonium	1.61	0.84	0.86	0.94	1.51
	毛壳菌属Chaetomium	1.31	6.82	1.83	2.45	16.78
	曲霉菌属Aspergillus	1.04	1.09	0.78	0.93	1.59
	青霉属Penicillium	0.79	1.90	1.67	2.65	1.51
	腐质霉属Humicola	0.53	1.52	1.23	0.93	0.20
	爪甲白癣菌属Arthrographis	0.48	0.32	0.17	0.40	0.07
	柄孢壳属Zopfiella	0.30	0.17	0.45	0.28	0.14
	赛多孢子菌属Scedosporium	0.32	0.76	0.95	0.35	0.17
	木霉属Trichoderma	0.36	0.33	0.83	0.96	1.37
	Phaeoacremonium	0.22	0.25	0.06	0.09	0.16
	Balsamia	0.49	0.19	<0.01	<0.01	<0.01
	球形密格孢属Junewangia	0.20	0.18	0.12	0.08	0.10
	篮状菌属Talaromyces	0.19	0.17	0.03	0.28	<0.01
	德巴利酵母属Debaryomyces	0.20	0.16	<0.01	0.01	0.02
	拟青霉属Paecilomyces	0.45	0.61	0.43	0.75	0.43
	Pseudaleuria	0.40	0.14	0.45	<0.01	<0.01
	头梗霉属Cephaliophora	0.15	0.12	<0.01	<0.01	0.01
	Xylomyces	0.12	0.12	0.13	0.18	<0.01
	Polyschema	0.11	1.49	0.09	0.01	0.01
	赭霉属Ochroconis	0.14	0.11	0.03	0.02	0.03
	节丛孢属Arthrobotrys	0.62	0.68	0.27	0.12	2.96
	假散囊菌属Pseudeurotium	0.11	0.11	0.07	0.14	0.11
菌门Phylum	菌属Species	CK	T1	T2	T3	T4
	柱霉属Scytalidium	0.10	0.09	0.25	0.07	0.02
	嗜热链球菌属Mycothermus	0.06	0.03	0.20	0.07	0.03
	Corallomycetella	0.03	0.04	0.03	0.18	0.10
	嗜热真菌属Thermomyces	0.02	0.03	0.26	0.04	0.04
	小画线壳属Monographella	0.02	0.03	0.03	0.01	0.25
	单胞瓶霉属Phialemonium	0.01	0.01	0.12	0.06	<0.01
	微囊菌属Microascus	0.11	0.01	0.14	<0.01	0.04
	柄孢壳菌属Podospora	0.11	0.01	0.02	0.01	0.10
	枝鼻菌属Cladorrhinum	0.21	0.01	0.12	0.20	0.07
	Menispora	<0.01	<0.01	<0.01	0.01	0.14
	Sagenomella	<0.01	<0.01	<0.01	0.05	0.21
	Mycoleptodiscus	0.10	<0.01	<0.01	<0.01	0.11
担子菌门Basidiomycota	蜡质菌属Ceriporia	0.91	0.98	11.76	5.21	<0.01
	锁瑚菌属Clavulina	0.46	0.11	0.04	0.21	0.07
	小脆柄菇属Psathyrella	0.33	0.18	0.25	0.42	0.23
	锥盖伞属Conocybe	0.33	0.04	0.20	0.63	0.78
	Waitea	<0.01	0.26	<0.01	0.00	<0.01
	齿菌属Hydnum	0.16	0.24	0.02	0.02	0.10
	肉齿菌属Sarcodon	0.16	0.13	<0.01	0.05	0.02
	Micropsalliota	<0.01	0.72	0.82	<0.01	<0.01
	Tricholosporum	0.09	0.07	<0.01	<0.01	0.25
	Arachnion	0.06	0.06	0.07	0.13	<0.01
	牛肝菌属Chalciporus	0.03	0.04	0.04	0.11	0.01
	Fibulochlamys	0.02	0.02	0.02	0.03	0.12
	拟鬼伞属Coprinopsis	0.16	0.01	0.01	0.01	0.10
	裸伞属Gymnopilus	0.55	1.68	<0.01	<0.01	0.11
	Globulicium	0.20	<0.01	<0.01	<0.01	0.44
	Biatoropsis	0.10	<0.01	0.02	0.51	<0.01
壶菌门Chytridiomycota	小壶菌属Spizellomyces	0.61	1.32	0.39	0.41	0.13
	根囊壶菌属Rhizophlyctis	0.50	0.15	0.21	0.87	1.33
	油壶菌属Olpidium	0.12	0.13	0.01	0.04	0.17
	囊壶菌属Phlyctochytrium	0.01	0.01	<0.01	<0.01	0.56
	Triparticalcar	<0.01	<0.01	0.02	0.02	0.15
接合菌门Zygomycota	被孢霉属Mortierella	16.22	8.71	6.20	4.64	5.46
	长孢菌属Ramicandelaber	0.13	0.05	1.82	0.87	<0.01

菌门Phylum	菌属Species	CK	T1	T2	T3	T4
子囊菌门Ascomycota	镰刀菌属Fusarium	5.77	2.74	1.88	1.05	0.47
	梭孢壳属Thielavia	5.14	3.95	3.71	4.90	1.86
	棒囊壳属Corynascus	3.28	2.32	2.91	7.73	2.55
	轮枝菌属Verticillium	2.45	1.29	0.99	0.88	0.40
	脉孢菌属Neurospora	1.63	1.66	5.66	2.69	1.19
	枝顶孢霉属Acremonium	1.61	0.84	0.86	0.94	1.51
	毛壳菌属Chaetomium	1.31	6.82	1.83	2.45	16.78
	曲霉菌属Aspergillus	1.04	1.09	0.78	0.93	1.59
	青霉属Penicillium	0.79	1.90	1.67	2.65	1.51
	腐质霉属Humicola	0.53	1.52	1.23	0.93	0.20
	爪甲白癣菌属Arthrographis	0.48	0.32	0.17	0.40	0.07
	柄孢壳属Zopfiella	0.30	0.17	0.45	0.28	0.14
	赛多孢子菌属Scedosporium	0.32	0.76	0.95	0.35	0.17
	木霉属Trichoderma	0.36	0.33	0.83	0.96	1.37
	Phaeoacremonium	0.22	0.25	0.06	0.09	0.16
	Balsamia	0.49	0.19	<0.01	<0.01	<0.01
	球形密格孢属Junewangia	0.20	0.18	0.12	0.08	0.10
	篮状菌属Talaromyces	0.19	0.17	0.03	0.28	<0.01
	德巴利酵母属Debaryomyces	0.20	0.16	<0.01	0.01	0.02
	拟青霉属Paecilomyces	0.45	0.61	0.43	0.75	0.43
	Pseudaleuria	0.40	0.14	0.45	<0.01	<0.01
	头梗霉属Cephaliophora	0.15	0.12	<0.01	<0.01	0.01
	Xylomyces	0.12	0.12	0.13	0.18	<0.01
	Polyschema	0.11	1.49	0.09	0.01	0.01
	赭霉属Ochroconis	0.14	0.11	0.03	0.02	0.03
	节丛孢属Arthrobotrys	0.62	0.68	0.27	0.12	2.96
	假散囊菌属Pseudeurotium	0.11	0.11	0.07	0.14	0.11
菌门Phylum	菌属Species	CK	T1	T2	T3	T4
	柱霉属Scytalidium	0.10	0.09	0.25	0.07	0.02
	嗜热链球菌属Mycothermus	0.06	0.03	0.20	0.07	0.03
	Corallomycetella	0.03	0.04	0.03	0.18	0.10
	嗜热真菌属Thermomyces	0.02	0.03	0.26	0.04	0.04
	小画线壳属Monographella	0.02	0.03	0.03	0.01	0.25
	单胞瓶霉属Phialemonium	0.01	0.01	0.12	0.06	<0.01
	微囊菌属Microascus	0.11	0.01	0.14	<0.01	0.04
	柄孢壳菌属Podospora	0.11	0.01	0.02	0.01	0.10
	枝鼻菌属Cladorrhinum	0.21	0.01	0.12	0.20	0.07
	Menispora	<0.01	<0.01	<0.01	0.01	0.14
	Sagenomella	<0.01	<0.01	<0.01	0.05	0.21
	Mycoleptodiscus	0.10	<0.01	<0.01	<0.01	0.11
担子菌门Basidiomycota	蜡质菌属Ceriporia	0.91	0.98	11.76	5.21	<0.01
	锁瑚菌属Clavulina	0.46	0.11	0.04	0.21	0.07
	小脆柄菇属Psathyrella	0.33	0.18	0.25	0.42	0.23
	锥盖伞属Conocybe	0.33	0.04	0.20	0.63	0.78
	Waitea	<0.01	0.26	<0.01	0.00	<0.01
	齿菌属Hydnum	0.16	0.24	0.02	0.02	0.10
	肉齿菌属Sarcodon	0.16	0.13	<0.01	0.05	0.02
	Micropsalliota	<0.01	0.72	0.82	<0.01	<0.01
	Tricholosporum	0.09	0.07	<0.01	<0.01	0.25
	Arachnion	0.06	0.06	0.07	0.13	<0.01
	牛肝菌属Chalciporus	0.03	0.04	0.04	0.11	0.01
	Fibulochlamys	0.02	0.02	0.02	0.03	0.12
	拟鬼伞属Coprinopsis	0.16	0.01	0.01	0.01	0.10
	裸伞属Gymnopilus	0.55	1.68	<0.01	<0.01	0.11
	Globulicium	0.20	<0.01	<0.01	<0.01	0.44
	Biatoropsis	0.10	<0.01	0.02	0.51	<0.01
壶菌门Chytridiomycota	小壶菌属Spizellomyces	0.61	1.32	0.39	0.41	0.13
	根囊壶菌属Rhizophlyctis	0.50	0.15	0.21	0.87	1.33
	油壶菌属Olpidium	0.12	0.13	0.01	0.04	0.17
	囊壶菌属Phlyctochytrium	0.01	0.01	<0.01	<0.01	0.56
	Triparticalcar	<0.01	<0.01	0.02	0.02	0.15
接合菌门Zygomycota	被孢霉属Mortierella	16.22	8.71	6.20	4.64	5.46
	长孢菌属Ramicandelaber	0.13	0.05	1.82	0.87	<0.01

Effects of different soil disinfection methods on soil fungal diversity and community structure

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[6]	GUI Guohong, YANG Hua, ZHU Jiangqun, ZHU Jianfeng, XIAO Yingping, XU E. Study on microbial community structure in chilled chicken during cold storage [J]. , 2019, 31(1): 47-55.
[7]	WU Yuefu, GU Aixing, WANG Hongkai. Isolation and identification of halophilic and halotolerant fungi from roots of Suaeda plants [J]. , 2018, 30(4): 649-655.
[8]	CHEN Yun, LIU Qi, DENG Junliang, YANG Yanyi, GAO Shuang, CHEN Chong, YAO Shuhua. Effects of composite antimicrobial peptide on rumen bacteria community structure of goat [J]. , 2017, 29(11): 1800-1808.
[9]	LI Yuan-yuan, SHI Kai, DELIGEER. Community structure and fauna of Lygus complex in Inner Mongolia [J]. , 2016, 28(9): 1558-1563.
[10]	ZHANG Aiju1，2，3， LIU Jindian1，2，3,，YANG Yuanjie1，2，3，GUO Aihuan1，2，3， GU Zhimin1，2，3，. Analysis of community characteristics of macrozoobenthos in enhancement and releasing zone in Tonglu section of Qiantang River [J]. , 2016, 28(8): 1323-.
[11]	LIU Dan, WU Feng-zhi. Effect of phenylalanine ammonialyase transgenic Arabidopsis thaliana on bacterial community in rhizosphere soil [J]. , 2016, 28(12): 2068-2075.
[12]	WANG Yan-yun, GUO Du-fa. Soil fungal diversity and its relationship with soil physical and chemical properties in saline alkali soil of Yellow River Delta [J]. , 2016, 28(11): 1901-1907.
[13]	HAN Li-na, DING Zhe-li, ZENG Hui-cai, ZHENG Wei, HE Ying-dui, GE Yu. Effect of functional organic fertilizer on growth of Chinese cabbage [J]. , 2016, 28(10): 1718-1723.
[14]	YU You-ping;CAI Xin-zhong;ZHU Xiao-xiang;*. Comparative analysis of microbial community structures in soils from rice-upland crop rotation fields by PLFA profile technique [J]. , 2013, 25(5): 0-1061.