Comparison of tolerance to high temperature of excellent strains of Sargassum fusiforme

doi:10.3969/j.issn.1004-1524.20221808

Acta Agriculturae Zhejiangensis ›› 2024, Vol. 36 ›› Issue (1): 148-155.DOI: 10.3969/j.issn.1004-1524.20221808

• Horticultural Science • Previous Articles Next Articles

Comparison of tolerance to high temperature of excellent strains of Sargassum fusiforme

ZUO Xiaojie(), WU Mingjiang, LUO Lin, MA Zengling, PANG Guanfeng, CHEN Binbin()

a. College of Life and Environmental Science, Wenzhou University,Wenzhou 325035, Zhejiang, China
b. Zhejiang Provincial Key Lab for Subtropical Water Environment and Marine Biological Resources Protection, Wenzhou University,Wenzhou 325035, Zhejiang, China

Received:2022-12-22 Online:2024-01-25 Published:2024-02-18

Abstract

Abstract:

Three excellent strains of Sargassum fusiforme and wild-type S. fusiforme were cultured at 25 ℃ to compare the growth status of S. fusiforme under high temperature conditions, and to provided data support for the screening of high temperature resistance strains. The results showed that long-term high temperature culture could reduce the photosynthetic performance of each strain of S. fusiforme, inhibit the activity of nitrate nitrogen absorption and assimilation related enzymes, reduce the content of soluble protein and soluble carbohydrate, enhance its dark respiration rate, and ultimately inhibit the growth of algae. Under high temperature condition, compared with other strains, the maximum electron transfer rate and saturated light intensity oxygen release rate of spindle vesicle type S. fusiforme were the highest, the peroxidase activity was the highest and increased significantly, while its malondialdehyde content did not increase significantly. In general, spindle vesicle type of S. fusiforme was less affected by high temperature and had strong tolerance. Spindle vesicle type of S. fusiforme could be popularized in actual breeding production, and the research results could provide a basis for subsequent variety improvement.

Key words: Sargassum fusiforme, seedlings breeding, high temperature stress, photosynthesis, seaweed aquaculture

CLC Number:

S968.425

ZUO Xiaojie, WU Mingjiang, LUO Lin, MA Zengling, PANG Guanfeng, CHEN Binbin. Comparison of tolerance to high temperature of excellent strains of Sargassum fusiforme[J]. Acta Agriculturae Zhejiangensis, 2024, 36(1): 148-155.

Figures/Tables 5

Fig.1 Morphological and relative growth rate of different Sargassum fusiforme strains under 20 ℃ and 25 ℃ A is the morphological change, B and C are relative growth rates of S. fusiforme strains cultured at 20 ℃ and 25 ℃, respectively. WT is wild type of S. fusiforme, SF-1 is round vesicle type of S. fusiforme, SF-2 is slender vesicle type of S. fusiforme, and SF-3 is spindle vesicle type of S. fusiforme. The same as below.

Fig.2 Photosynthesis characteristics of different Sargassum fusiforme strains under 20 ℃ and 25 ℃ Data on the bars marked without the same letter indicated significant differences at P<0.05. *, Represents the differences in various indicators of differen S. fusiforme strains under the same temperature treatment (*, P <0.05; **, P <0.01). The same as below.

Fig.3 Nitrate absorption and utilization characteristics of S. fusiforme strains under 20 ℃ and 25 ℃

Fig.4 Soluble protein and soluble carbohydrate content of different S. fusiforme strains under 20 ℃ and 25 ℃

Fig.5 Superoxide dismutase activity, peroxidase activity and malondialdehyde content of different S. fusiforme strains under 20 ℃ and 25 ℃

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Comparison of tolerance to high temperature of excellent strains of Sargassum fusiforme

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