Response of leaf water status, photosynthetic and fluorescence parameters to different irrigation lower limits in substrate culture of cucumber

doi:10.3969/j.issn.1004-1524.2020.12.07

Acta Agriculturae Zhejiangensis ›› 2020, Vol. 32 ›› Issue (12): 2162-2172.DOI: 10.3969/j.issn.1004-1524.2020.12.07

• Horticultural Science • Previous Articles Next Articles

Response of leaf water status, photosynthetic and fluorescence parameters to different irrigation lower limits in substrate culture of cucumber

JIN Ning(), LYU Jian^*(), YU Jihua, JIN Li, ZHANG Guobin, XIAO Xuemei, HU Linli

College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China

Received:2020-05-07 Online:2020-12-25 Published:2020-12-25
Contact: LYU Jian

Abstract

Abstract:

In order to find out the suitable lower limit of irrigation and realize agricultural water-saving, the cucumber variety Bote 209 was used as material in this experiment, and four irrigation water limit treatments were set in potted medium, 50%, 60%, 70%and 80% of field water holding capacity, which were represented by A, B, C and D, respectively. The upper limit of irrigation was set to 90% of field water holding capacity. TDR350 water velocimeter was used to control the water content of the substrate.The effects of different irrigation lower limit on water status, diurnal variation of photosynthesis and fluorescence parameters of cucumber leaves were studied.The results showed that the relative water content, free water content and free water/bound water of leaves increased gradually with the increase of irrigation lower limit, while the water saturation deficit, leaf cell sap concentration and bound water content showed a reverse trend.The relative water content and free water content of treatment C and D were significantly (P<0.05) higher than those of treatment A and B.The leaf cell sap concentration of treatment A was significantly(P<0.05) higher than those of treatment C and D, and the bound water content of treatment A and B was significantly (P<0.05)higher than treatment C and D. The content of chlorophyll a, chlorophyll b and chlorophyll a+b increased firstly and then decreased with the increase of irrigation lower limit, while the content of carotenoids decreased firstly and then increased. The content of chlorophyll a and chlorophyll a+b in treatment C was significantly(P<0.05)higher than that in treatment A and B.The change trend of C_i in treatment C and D was showed as V-shape, while that in treatment A and B was W-shaped.T_r in treatment A, B, C, D firstly increased and then declined, which showed a trend of gradually declining after 14:00 in treatment B, C, D, and a trend of rapid decline after 12:00 in treatment A. G_s showed a double peak curve of “M” type in treatment B, C, D, and a single peak curve in treatment A. The change trend of P_n in treatment A, B, C, D leaves were similar as G_s. During the whole process of daily change, P_n in treatment C and D were always higher than that in treatment A and B. F_v/F_m, Φ_PSⅡ, qP increased with the increase of irrigation lower limit, while NPQ decreased.It was concluded that the lower limit of irrigation was 70%-80% of the field water capacity. In this range, the water content of cucumber leaves under substrate cultivation was good, the content of photosynthetic pigment was high, and the net photosynthetic rate was high, which was beneficial to the photosynthesis and the growth and development of cucumber.

Key words: cucumber, irrigation lower limit, diurnal variation of photosynthesis, fluorescence parameters

CLC Number:

S642.2

JIN Ning, LYU Jian, YU Jihua, JIN Li, ZHANG Guobin, XIAO Xuemei, HU Linli. Response of leaf water status, photosynthetic and fluorescence parameters to different irrigation lower limits in substrate culture of cucumber[J]. Acta Agriculturae Zhejiangensis, 2020, 32(12): 2162-2172.

Figures/Tables 6

References 47

[1]	UNCCD. National report based on the United Nations convention to combat desertification in those countries experiencing serious drought and/ordesertification particularly in Africa. Paris[EB/OL]. [ 2020-05-07]. http://www.env.go.jp/press/files/jp1677.pdf.
[2]	黄萌田, 周佰铨, 翟盘茂. 极端天气气候事件变化对荒漠化、土地退化和粮食安全的影响[J]. 气候变化研究进展, 2020,16(1):17-27.
	HUANG M T, ZHOU B Q, ZHAI P M. Impacts of extreme weather and climate events on desertification, land degradation and food security[J]. Climate Change Research, 2020,16(1):17-27.(in Chinese with English abstract)
[3]	陈克恭, 师安隆. 戈壁农业是生态文明背景下的农业革命探索[J]. 农业经济问题, 2019,40(5):130-137.
	CHEN K G, SHI A L. Desert farming: exploration of agricultural revolution in the ecological civilization setting[J]. Issues in Agricultural Economy, 2019,40(5):130-137.(in Chinese with English abstract)
[4]	张宪法, 于贤昌, 张振贤. 土壤水分对温室嫁接和非嫁接黄瓜生长与生理特性的影响[J]. 应用生态学报, 2002,13(11):1399-1402. PMID
	ZHANG X F, YU X C, ZHANG Z X. Effect of soil water on the growth and physiological characteristics of grafted and non-grafted cucumber in greenhouse[J]. Chinese Journal of Applied Ecology, 2002,13(11):1399-1402.(in Chinese with English abstract) URL PMID
[5]	李建明, 邹志荣. 灌溉土壤水分上限对温室番茄开花坐果期生理指标的影响[J]. 西北农业学报, 2000,9(4):71-74.
	LI J M, ZOU Z R. Effects of different soil water irrigation maximum on physiological indexes of greenhouse tomato at flowering and fruit-bearing periods[J]. Acta Agriculturae Boreali-Occidentalis Sinica, 2000,9(4):71-74.(in Chinese with English abstract)
[6]	GALLÉ A, HALDIMANN P, FELLER U. Photosynthetic performance and water relations in young pubescent oak (Quercuspubescens) trees during drought stress and recovery[J]. New Phytologist, 2007,174(4):799-810. DOI URL
[7]	魏爱丽, 王志敏, 翟志席, 等. 土壤干旱对小麦旗叶和穗器官C4光合酶活性的影响[J]. 中国农业科学, 2003,36(5):508-512.
	WEI A L, WANG Z M, ZHAI Z X, et al. Effect of soil drought on C4 photosynjournal enzyme activities of flag leaf and ear in wheat[J]. Scientia Agricultura Sinica, 2003,36(5):508-512.(in Chinese with English abstract)
[8]	FLEXAS J, BOTA J, GALMÉS J, et al. Keeping a positive carbon balance under adverse conditions: responses of photosynjournal and respiration to water stress[J]. Physiologia Plantarum, 2006,127(3):343-352. DOI URL
[9]	孙东宝, 王庆锁. 水分对苜蓿叶片光合特性的影响[J]. 植物生态学报, 2012,36(1):72-80. DOI URL
	SUN D B, WANG Q S. Effects of water on the photosynthetic characteristics of alfalfa (Medicago sativa)[J]. Chinese Journal of Plant Ecology, 2012,36(1):72-80.(in Chinese with English abstract) DOI URL
[10]	刘明, QI H, 孙世贤, 等. 水分胁迫对玉米光合特性的影响[J]. 玉米科学, 2008,16(4):86-90.
	LIU M, QI H, SUN S X, et al. Effect of water stress on maize photosynthetic characteristics[J]. Journal of Maize Sciences, 2008,16(4):86-90.(in Chinese with English abstract)
[11]	张仁和, 郑友军, 马国胜, 等. 干旱胁迫对玉米苗期叶片光合作用和保护酶的影响[J]. 生态学报, 2011,31(5):1303-1311.
	ZHANG R H, ZHENG Y J, MA G S, et al. Effects of drought stress on photosynthetic traits and protective enzyme activity in maize seeding[J]. Acta Ecologica Sinica, 2011,31(5):1303-1311.(in Chinese with English abstract)
[12]	韩国君, 陈年来, 黄海霞, 等. 番茄叶片光合作用对快速水分胁迫的响应[J]. 应用生态学报, 2013,24(4):1017-1022. URL PMID
	HAN G J, CHEN N L, HUANG H X, et al. Responses of tomato leaf photosynjournal to rapid water stress[J]. Chinese Journal of Applied Ecology, 2013,24(4):1017-1022.(in Chinese with English abstract) URL PMID
[13]	ZHANG S Y, ZHANG G C, GU S Y, et al. Critical responses of photosynthetic efficiency of goldspur apple tree to soil water variation in semiarid loess hilly area[J]. Photosynthetica, 2010,48(4):589-595. DOI URL
[14]	丛雪, 齐华, 孟凡超, 等. 干旱胁迫对玉米叶绿素荧光参数及质膜透性的影响[J]. 华北农学报, 2010,25(5):141-144. DOI URL
	CONG X, QI H, MENG F C, et al. Effects of water strsses on chlorophyll fluorescence parameters and membrane permeability of different maize varieties[J]. Acta Agriculturae Boreali-Sinica, 2010,25(5):141-144.(in Chinese with English abstract) DOI URL
[15]	须晖, 高洁, 王蕊, 等. 番茄幼苗叶绿素荧光参数对水分胁迫的响应[J]. 中国农学通报, 2011,27(10):189-193.
	XU H, GAO J, WANG R, et al. Response of water stress on chlorophyll fluorescence parameters of tomato seedlings[J]. Chinese Agricultural Science Bulletin, 2011,27(10):189-193.(in Chinese with English abstract)
[16]	肖文静, 孙建磊, 王绍辉, 等. 适度水分胁迫提高黄瓜幼苗光合作用弱光适应性[J]. 园艺学报, 2010,37(9):1439-1448.
	XIAO W J, SUN J L, WANG S H, et al. Moderate water stress increased the photosynjournal adaptability of cucumber seedlings under low light[J]. Acta Horticulturae Sinica, 2010,37(9):1439-1448.(in Chinese with English abstract)
[17]	REYNOLDS M P, ACEVEDO E, SAYRE K D, et al. Yield potential in modern wheat varieties: its association with a less competitive ideotype[J]. Field Crops Research, 1994,37(3):149-160. DOI URL
[18]	李琨, 郁继华, 颉建明, 等. 不同灌水下限对日光温室有机生态型无土栽培辣椒生长指标的影响[J]. 甘肃农业大学学报, 2011,46(2):41-44.
	LI K, YU J H, XIE J M, et al. Effects of different lower limit of drip irrigation quantity on growth of green pepper under ecological organic soilless culture in greenhouse[J]. Journal of Gansu Agricultural University, 2011,46(2):41-44.(in Chinese with English abstract)
[19]	邹琦. 植物生理学实验指导[M]. 北京: 中国农业出版社, 2000.
[20]	高俊凤. 植物生理学实验指导[M]. 北京: 高等教育出版社, 2006.
[21]	王翠丽. 不同施肥方式对辣椒生长生理和养分利用的影响[D]. 兰州: 甘肃农业大学, 2017.
	WANG C L. Effect of different fertilizer methods on growth physiology and nutrient utilization of pepper[D]. Lanzhou: Gansu Agricultural University, 2017.(in Chinese with English abstract)
[22]	徐俊增, 彭世彰, 魏征, 等. 节水灌溉水稻叶片胞间CO₂浓度及气孔与非气孔限制[J]. 农业工程学报, 2010,26(7):76-80.
	XU J Z, PENG S Z, WEI Z, et al. Intercellular CO₂ concentration and stomatal or non-stomatal limitation of rice under water saving irrigation[J]. Transactions of the Chinese Society of Agricultural Engineering, 2010,26(7):76-80.(in Chinese with English abstract)
[23]	DAVIES W J, ZHANG J. Root signals and the regulation of growth and development of plants in drying soil[J]. Annual Review of Plant Physiology and Plant Molecular Biology, 1991,42(1):55-76. DOI URL
[24]	郝树荣, 郭相平, 张展羽. 作物干旱胁迫及复水的补偿效应研究进展[J]. 水利水电科技进展, 2009,29(1):81-84.
	HAO S R, GUO X P, ZHANG Z Y. Research progress on compensatory effects at crops in drought stress and rehydration[J]. Advances in Science and Technology of Water Resources, 2009,29(1):81-84.(in Chinese with English abstract)
[25]	CHAVES M M, PEREIRA J S, JP M, et al. How plants cope with water stress in the field? photosynjournal and growth[J]. Annals of Botany, 2002,89:907-916. URL PMID
[26]	康绍忠, 杜太生, 孙景生, 等. 基于生命需水信息的作物高效节水调控理论与技术[J]. 水利学报, 2007,38(6):661-667.
	KANG S Z, DU T S, SUN J S, et al. Theory and technology of improving irrigation water use efficiency based on crop growing water demand information[J]. Journal of Hydraulic Engineering, 2007,38(6):661-667.(in Chinese with English abstract)
[27]	曹正鹏, 刘玉汇, 张小静, 等. 亏缺灌溉对马铃薯生长产量及水分利用的影响[J]. 农业工程学报, 2019,35(4):114-123.
	CAO Z P, LIU Y H, ZHANG X J, et al. Effects of deficit irrigation on growth, yield and water use of potato plants[J]. Transactions of the Chinese Society of Agricultural Engineering, 2019,35(4):114-123.(in Chinese with English abstract)
[28]	刘小飞, 费良军, 段爱旺, 等. 调亏灌溉对冬小麦产量和品质及其关系的调控效应[J]. 水土保持学报, 2019,33(3):276-282.
	LIU X F, FEI L J, DUAN A W, et al. Effect of regulated deficit irrigation (RDI) on grain yield and quality traits of winter wheat and their relationship[J]. Journal of Soil and Water Conservation, 2019,33(3):276-282.(in Chinese with English abstract)
[29]	李彩霞, 周新国, 孙景生, 等. 交替隔沟灌溉下玉米根长密度分布及水分利用[J]. 灌溉排水学报, 2012,31(6):81-83.
	LI C X, ZHOU X G, SUN J S, et al. Distribution of root length density and water use efficiency of maize with alternative furrow irrigation[J]. Journal of Irrigation and Drainage, 2012,31(6):81-83.(in Chinese with English abstract)
[30]	MANGALASSERY S, REJANI R, SINGH V, et al. Impact of different irrigation regimes under varied planting density on growth, yield and economic return of cashew (Anacardium occidentale L.)[J]. Irrigation Science, 2019,37(4):483-494. DOI URL
[31]	SÁNCHEZ-RODRÍGUEZ E, RUBIO-WILHELMI M, CERVILLA L M, et al. Genotypic differences in some physiological parameters symptomatic for oxidative stress under moderate drought in tomato plants[J]. Plant Science, 2010,178(1):30-40. DOI URL
[32]	GHASSEMIGOLEZANI K, BAKHSHY J, ZEHTABSALMASI S, et al. Changes in leaf characteristics and grain yield of soybean (Glycine max L.) in response to shading and water stress[J]. International Journal of Biosciences, 2013,3(2):71-79.
[33]	鲍思伟. 水分胁迫对蚕豆(Vicia faba L.)叶片水分状况的影响[J]. 天津师范大学学报(自然科学版), 2001,21(3):67-70.
	BAO S W. Effects of water stress on the state of water in Vicia faba L. leaves[J]. Journal of Tianjin Normal University (Natural Science Edition), 2001,21(3):67-70.(in Chinese with English abstract)
[34]	柏成寿, 陆帼一. 水分胁迫对番茄幼苗生长影响的研究[J]. 园艺学报, 1991,18(4):340-344.
	BAI C S, LU G Y. Effects of water stress on tomato (Lycopersicon esculentum Mill.) seedling[J]. Acta Horticulturae Sinica, 1991,18(4):340-344.(in Chinese with English abstract)
[35]	吕剑. 日光温室基质栽培越冬茬番茄灌水下限研究[D]. 兰州: 甘肃农业大学, 2012.
	LYU J. The study of irrigation limits of overwintering tomato in heliogreenhouse under substrate culture[D]. Lanzhou: Gansu Agricultural University, 2012.(in Chinese with English abstract)
[36]	李银坤, 武雪萍, 吴会军, 等. 水氮条件对温室黄瓜光合日变化及产量的影响[J]. 农业工程学报, 2010,26(增刊):122-129.
	LI Y K, WU X P, WU H J, et al. Effects of water and nitrogen conditions on the diurnal variation of photosynjournal and yield of cucumber in greenhouse[J]. Transactions of the Chinese Society of Agricultural Engineering, 2010,26(Supp.):122-129.(in Chinese with English abstract)
[37]	邢英英, 张富仓, 张燕, 等. 滴灌施肥水肥耦合对温室番茄产量、品质和水氮利用的影响[J]. 中国农业科学, 2015,48(4):713-726. DOI URL
	XING Y Y, ZHANG F C, ZHANG Y, et al. Effect of irrigation and fertilizer coupling on greenhouse tomato yield, quality, water and nitrogen utilization under fertigation[J]. Scientia Agricultura Sinica, 2015,48(4):713-726.(in Chinese with English abstract) DOI URL
[38]	齐曼·尤努斯, 木合塔尔·扎热, 塔衣尔·艾合买提. 干旱胁迫下尖果沙枣幼苗的根系活力和光合特性[J]. 应用生态学报, 2011,22(7):1789-1795. URL PMID
	QIMAN Y, MUHTAR Z, TAYER A. Root activity and photosynthetic characteristics of Elaeagnus oxycarpa seedlings under drought stress[J]. Chinese Journal of Applied Ecology, 2011,22(7):1789-1795.(in Chinese with English abstract) URL PMID
[39]	吴顺, 张雪芹, 蔡燕. 干旱胁迫对黄瓜幼苗叶绿素含量和光合特性的影响[J]. 中国农学通报, 2014,30(1):133-137.
	WU S, ZHANG X Q, CAI Y. Effects of drought stress on chlorophyll contents and photosynthetic characteristics of cucumber seedlings[J]. Chinese Agricultural Science Bulletin, 2014,30(1):133-137.(in Chinese with English abstract)
[40]	翟胜, 梁银丽, 王巨媛, 等. 土壤水分对日光温室黄瓜生长发育及光合特性的影响[J]. 中国农学通报, 2005,21(2):187-191.
	ZHAI S, LIANG Y L, WANG J Y, et al. The effect of soil moisture on growth & development and photosynthetic characteristics of Cucumis statirus L. in solar greenhouse[J]. Chinese Agricultural Science Bulletin, 2005,21(2):187-191.(in Chinese with English abstract)
[41]	刘祖贵, 陈金平, 段爱旺, 等. 不同土壤水分处理对夏玉米叶片光合等生理特性的影响[J]. 干旱地区农业研究, 2006,24(1):90-95.
	LIU Z G, CHEN J P, DUAN A W, et al. Effects of different soil moisture treatments on physiological characteristics of summer maize leaves[J]. Agricultural Research in the Arid Areas, 2006,24(1):90-95.(in Chinese with English abstract)
[42]	刘帆, 申双和, 李永秀, 等. 不同生育期水分胁迫对玉米光合特性的影响[J]. 气象科学, 2013,33(4):378-383.
	LIU F, SHEN S H, LI Y X, et al. Effect of water stress on maize photosynthetic characteristics in different growth stages[J]. Journal of the Meteorological Sciences, 2013,33(4):378-383.(in Chinese with English abstract)
[43]	于文颖, 纪瑞鹏, 冯锐, 等. 不同生育期玉米叶片光合特性及水分利用效率对水分胁迫的响应[J]. 生态学报, 2015,35(9):2902-2909. DOI URL
	YU W Y, JI R P, FENG R, et al. Response of water stress on photosynthetic characteristics and water use efficiency of maize leaves in different growth stage[J]. Acta Ecologica Sinica, 2015,35(9):2902-2909.(in Chinese with English abstract)
[44]	FARQUHAR G D, SHARKEY T D. Stomatal conductance and photosynjournal[J]. Annual Review of Plant Physiology, 1982,33(1):317-345. DOI URL
[45]	高玉红, 牛俊义, 徐锐, 等. 不同覆膜方式对玉米叶片光合、蒸腾及水分利用效率的影响[J]. 草业学报, 2012,21(5):178-184. DOI URL
	GAO Y H, NIU J Y, XU R, et al. Effects of different film mulching on photosynjournal, transpiration rate and leaf water use efficiency of maize[J]. Acta Prataculturae Sinica, 2012,21(5):178-184.(in Chinese with English abstract) DOI URL
[46]	付秋实, 李红岭, 崔健, 等. 水分胁迫对辣椒光合作用及相关生理特性的影响[J]. 中国农业科学, 2009,42(5):1859-1866.
	FU Q S, LI H L, CUI J, et al. Effects of water stress on photosynjournal and associated physiological characters of Capsicum annuum L[J]. Scientia Agricultura Sinica, 2009,42(5):1859-1866.(in Chinese with English abstract)
[47]	路丙社, 白志英, 孙浩元, 等. 土壤含水量对阿月浑子叶片净光合速率及叶绿素荧光参数的影响[J]. 园艺学报, 2004,31(6):727-731.
	LU B S, BAI Z Y, SUN H Y, et al. Effects of soil water content level on net photosynthetic rate and chlorophyll fluorescence parameters in pistachio leaves[J]. Acta Horticulturae Sinica, 2004,31(6):727-731.(in Chinese with English abstract)

处理 Treatment	田间持水量 Field water holding capacity/%	每盆灌水量 Irrigation amount per pot/L
A	50	1.35
B	60	1.01
C	70	0.68
D	80	0.34

处理 Treatment	田间持水量 Field water holding capacity/%	每盆灌水量 Irrigation amount per pot/L
A	50	1.35
B	60	1.01
C	70	0.68
D	80	0.34

处理	相对含水量	水分饱和亏	细胞汁液浓度	自由水	束缚水	自由水/束缚水
Treatment	RWC/%	WSD/%	Cell sap concentrations/%	Free water/%	Bound water/%	Free water/ Bound water
A	78.463 ± 0.093 b	21.537 ± 0.093a	14.325 ± 0.562 a	66.846 ± 0.721c	17.080 ± 0.436 a	3.917 ± 0.081 c
B	80.021 ± 0.246 b	19.979 ± 0.246 a	13.875 ± 0.404 ab	72.980 ± 1.488 b	10.477 ± 2.171 b	7.876 ± 2.205 bc
C	82.806 ± 0.973 a	17.194 ± 0.973 b	12.750 ± 0.504 bc	78.801 ± 1.671 a	5.594 ± 0.942 c	14.989 ± 2.775ab
D	84.220 ± 0.534 a	15.780 ± 0.534 b	12.425 ± 0.321 c	83.218 ± 1.442 a	5.102 ± 1.027 c	17.672 ± 3.401 a

处理	相对含水量	水分饱和亏	细胞汁液浓度	自由水	束缚水	自由水/束缚水
Treatment	RWC/%	WSD/%	Cell sap concentrations/%	Free water/%	Bound water/%	Free water/ Bound water
A	78.463 ± 0.093 b	21.537 ± 0.093a	14.325 ± 0.562 a	66.846 ± 0.721c	17.080 ± 0.436 a	3.917 ± 0.081 c
B	80.021 ± 0.246 b	19.979 ± 0.246 a	13.875 ± 0.404 ab	72.980 ± 1.488 b	10.477 ± 2.171 b	7.876 ± 2.205 bc
C	82.806 ± 0.973 a	17.194 ± 0.973 b	12.750 ± 0.504 bc	78.801 ± 1.671 a	5.594 ± 0.942 c	14.989 ± 2.775ab
D	84.220 ± 0.534 a	15.780 ± 0.534 b	12.425 ± 0.321 c	83.218 ± 1.442 a	5.102 ± 1.027 c	17.672 ± 3.401 a

Response of leaf water status, photosynthetic and fluorescence parameters to different irrigation lower limits in substrate culture of cucumber

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 6

References 47

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	NIU Bo, LI Lina, PANG Guangchang, LU Dingqiang. Construction of plant root tip mesenchymal sensors and their effects on urea sensing dynamics [J]. , 2020, 32(8): 1466-1474.
[2]	ZHU Senlin, WANG Danmei, TANG Xiumei, CHEN Rui, YANG Rong, XUE Yuemei, JIN Luyi, REN Qingwen, LIU Peng, LUO Jun. Optimization of culture conditions of Trichoderma water dispersing granule and its biocontrol effects on cucumber fusarium wilt [J]. , 2020, 32(6): 1009-1018.
[3]	LIN Hui, ZHANG Jin, YUAN Qianyu, YE Jing, SUN Wanchun, YU Yijun, YU Qiaogang, MA Junwei. Improving microbial system of continuous cropping soil by addition of Trichoderma asperellum and ultrafine powder humus [J]. , 2020, 32(6): 1060-1069.
[4]	JIN Ning, LYU Jian, YU Jihua, JIN Li, ZHANG Guobin, XIAO Xuemei, HU Linli. Response of leaf water status, photosynthetic and fluorescence parameters to different irrigation lower limits in substrate culture of cucumber [J]. Acta Agriculturae Zhejiangensis, 2020, 32(12): 2162-2172.
[5]	WANG Yanyun, ZHAO Longjie, HAO Chunli, CAI Jinzhong. Effects of bio-organic fertilizer on soil microbial population and enzymes activities under different continuous cropping years of protected cucumber [J]. , 2019, 31(4): 631-638.
[6]	ZHOU Yanchao, WU Yanhong, TIAN Xingwu, ZHOU Haixia, HAN Zeyu, LIU Jiqing, LAN Zhiqian, ZHANG Xueyan. Effect of nano-carbon sol and Bacillus subtilis on cucumber growth and soil environment [J]. , 2019, 31(3): 392-400.
[7]	LI Qi, ZHAO Jie, YANG Liu, WANG Jun, GAO Yixing. Cucumber leaf lesion identification based on GA-BP neural network and feature vector optimization combination [J]. , 2019, 31(3): 487-495.
[8]	LI Yang, LIU Kai, WEI Jipeng, ZHANG Lan, LI Xin, HAN Wenyan, LI Qingyun. Effects of various concentrations of EGCG on seed germination and resistance in cucumber under NaCl stress [J]. , 2018, 30(7): 1160-1167.
[9]	HU Yuan, ZHANG Tao, CAI Jingbo, TANG Ming, LUO Kui, HU Lihua. Effect of culture density, size and depth layer on growth of Apostichopus japonicas in shallow cages [J]. , 2018, 30(4): 548-553.
[10]	ZHENG Xiang, JIANG Liangbo, DENG Bangliang, LIU Qian, LIU Xishuai, ZHENG Liya, GUO Xiaomin, LIU Yuanqiu, ZHANG Ling. Effects of enhanced UV-B radiation and nitrogen deposition on chlorophyll fluorescence parameters of invasive plant Triadica sebifera [J]. , 2018, 30(2): 248-254.
[11]	HU Zhihui, ZHANG Liqin, WANG Yanjie, LAN Hong, GUO Rui, CHEN Gao, CHEN Chanyou. Effects on spectral indexes and fluorescence parameters of cowpea leaves after spraying cytokinin [J]. , 2017, 29(6): 943-950.
[12]	XIANG Zhidan, ZHANG Zhenxiao, LI Chao, DU Zhiyou. Investigation of efficiency and stability of Cucumber mosaic virus-based gene silencing vector [J]. , 2017, 29(4): 625-630.
[13]	WANG Kelei, ZHOU Youhe, SHI Jianlei, HUANG Zong’an, ZHU Longjing, XU Jian. Application of dynamic water level management of ebb and flow irrigation for cucumber seedlings [J]. , 2017, 29(3): 408-413.
[14]	LU Jinda, LIAO Qiansheng, DU Zhiyou. Overcome the defect in virus replication conferred by heterogeneous combination of viral replicases through mutation and recombination [J]. , 2017, 29(3): 445-449.
[15]	ZHANG Xiaomei, HU Chaoyi, LIU Tao, ZHOU Yanhong. Effects of light quality on drought resistance of cucumber seedlings [J]. , 2017, 29(1): 58-63.