Investigation of efficiency and stability of Cucumber mosaic virus-based gene silencing vector

doi:10.3969/j.issn.1004-1524.2017.04.16

›› 2017, Vol. 29 ›› Issue (4): 625-630.DOI: 10.3969/j.issn.1004-1524.2017.04.16

• Plant Protection • Previous Articles Next Articles

Investigation of efficiency and stability of Cucumber mosaic virus-based gene silencing vector

XIANG Zhidan, ZHANG Zhenxiao, LI Chao, DU Zhiyou^*

College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, China

Received:2016-11-30 Online:2017-04-20 Published:2017-04-27

Abstract

Abstract: Virus-induced gene silencing (VIGS) has been widely used for analysis of plant gene function. In this work, using phytoene desaturase (PDS) as an indicator, efficiency of Cucumber mosaic virus(CMV) as a VIGS vector was investigated to silence PDS mRNA in Nicotiana benthamiana. Recombinant viruses were generated by replacing the 2b ORF with a set of PDS fragments, and tested in N. benthamiana plants. Results showed that these recombinants induced photobleaching phenotype in the upper systemic leaves and silencing efficiency was correlated with the length of the PDS inserts in some extents, but they did not cause obvious photobleaching phenotype in the newly top leaves and oversized inserts (≥518 nt) reduced genetic stability of viral genome, leading to partial deletion of the inserts. Thus, the optimal insert is about 350 nt in length for CMV-based VIGS vector.

Key words: virus-induced gene silencing, Cucumber mosaic virus, 2b, phytoene desaturase

CLC Number:

Q933

XIANG Zhidan, ZHANG Zhenxiao, LI Chao, DU Zhiyou. Investigation of efficiency and stability of Cucumber mosaic virus-based gene silencing vector[J]. , 2017, 29(4): 625-630.

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Investigation of efficiency and stability of Cucumber mosaic virus-based gene silencing vector

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