Cloning and bioinformatics analysis of MSTN gene of Tianzhu white yak

doi:10.3969/j.issn.1004-1524.2017.04.15

›› 2017, Vol. 29 ›› Issue (4): 618-624.DOI: 10.3969/j.issn.1004-1524.2017.04.15

• Plant Protection • Previous Articles Next Articles

Cloning and bioinformatics analysis of MSTN gene of Tianzhu white yak

ZHANG Li, LIU Lixia, LI Qiangzi, CHEN Hong

College of Life Science and Engineering, Northwest University for Nationalities, Lanzhou 730030, China

Received:2016-09-26 Online:2017-04-20 Published:2017-04-27

Abstract

Abstract: In order to elucidate the structure of MSTN gene, the complete MSTN gene sequence of Tianzhu white yak was amplified and analyzed through PCR, cloning, sequencing and the bioinformatics tools. The results showed that the sequence of MSTN gene was 1 128 bp in length and encoded 375 amino acids with a molecular weight of 42.55 ku and pI of 6.14. Bioinformatics prediction found that MSTN protein of Tianzhu white yak is unstable hydrophilic protein and mRNA secondary structure of minimum free energy is -1 135.16 kJ·mol^-1. Protein secondary and tertiary structure were based on extended strand and random coil-based mixed-type protein. The results laid a foundation for further studying on the genetic characteristics and physiological mechanisms of MSTN.

Key words: Tianzhu white yak, MSTN gene, clone, bioinformatics

CLC Number:

S823.8⁺5

ZHANG Li, LIU Lixia, LI Qiangzi, CHEN Hong. Cloning and bioinformatics analysis of MSTN gene of Tianzhu white yak[J]. , 2017, 29(4): 618-624.

References

[1] 乌日罕,付艳茹,格日勒图.肌肉生长抑制素基因(MSTN)的研究进展[J].畜牧与饲料科学, 2015,36(8):30-32.
WU R H, FU Y R, GERELT B. Research progress on myostatin gene (MSTN)[J]. Animal Husbandry and Feed Science , 2015,36(8):30-32.(in Chinese with English abstract)
[2] OLDHAM J M, MARTYN JA K, SHARMA M, et al. Molecular expression of myostatin and MyoD is greater in double-muscled than normal-muscled cattle fetuses[J]. American Journal of Physiology-Regulatory Integrative and Comparative Physiology , 2001, 280(5): 1488-1493.
[3] KAMBADUR R,SHARMA M,SMITH T P, et al. Mutationsin myostatin(GDF-8)in double-muscled Belgian blue and piedmontese cattle[J]. Genome Research , 1997, 7(9):910-916.
[4] 沈丽红,熊良伟,唐永凯,等.奥利亚罗非鱼MSTN基因结构及其SNPs的筛选[J].上海海洋大学学报,2010,19(2):157-161.
SHEN L H, XIONG L W, TANG Y K, et al. The gene structure and identification of single nucleotide polymorphisms of MSTN in Oreochrom is aureus[J]. Journal of Shanghai Ocean University ,2010,19(2):157-161. (in Chinese with English abstract)
[5] 毛亮, 徐亚欧, 麻丽霞. 藏鸡肌肉生长抑制素基因克隆及序列分析[J].西南民族大学学报(自然科学版),2009,35(4):772-775.
MAO L, XU Y O, MA L X. Study on cloning of the MSTN gene in the Tibetan gallus[J]. Journal of Southwest University for Nationalities ( Natural Science Edition ),2009,35(4):772-775. (in Chinese with English abstract)
[6] 巴恒星.东北马鹿 MSTN 基因克隆序列分析和多态性研究[D].北京:中国农业科学院, 2007.
BA H X. Cloning and sequence analysis of Manehurian wapiti MSTN gene and polymorphism studies[D].Beijing: Chinese Academy of Agricultural Sciences,2007. (in Chinese with English abstract)
[7] 关学敏, 张海燕, 杨晴,等.马MSTN蛋白的生物信息学分析[C]//Proceedings of 2010 First International Conference on Cellular,Molecular Biology, Biophysics and Bioengineering(Volume 5). 武汉:湖北省智能信息技术应用学会,2010.
[8] 杨海龙,黄向阳,王梦云,等.美洲黑貂 MSTN 基因的克隆及序列分析[J].黑龙江畜牧兽医, 2013(11):158-160.
YANG H L, HUANG X Y, WANG M Y, et al. Cloning and sequencing of the Americas sable MSTN gene[J]. Heilongjiang Animal Science and Veterinary , 2013(11): 158-160. (in Chinese)
[9] 金鑫燕.山羊肌生成抑制素基因的克隆及生物信息学分析[J].中国畜牧兽医, 2011, 38(9):111-114.
JIN X Y. Cloning and bioinformatics analysis of MSTN gene of goat[J]. China Animal Husbandry and Veterinary Medicine , 2011, 38(9):111-114. (in Chinese with English abstract)
[10] 王伟,连林生,李继中.猪 MSTN 基因生物信息学分析[J].安徽农业科学, 2012, 40(10):897-900.
WANG W, LIAN L S, LI J Z. Bioinformatics analysis of pig MSTN gene[J]. Anhui Agricultural Sciences , 2012, 40(10):897-900. (in Chinese with English abstract)
[11] 杜晓华,李积友,杨阳,等.甘肃西门塔尔牛高台类群 MSTN 基因多态性与生物信息学研究[J].畜牧兽医杂志, 2015, 34(1):13-17.
DU X H, LI J Y, YANG Y, et al. Polymorphism and Bioinformatics Analysis of MSTN Gene in Gaotai Simmental Cattle of Gansu[J]. Animal Husbandry and Veterinary Medicine , 2015, 34(1):13-17. (in Chinese with English abstract)
[12] 梁春年,丁学智,包鹏甲,等.牦牛 MSTN 基因分子克隆及序列分析[J].华北农学报, 2013, 28(1):76-81.
LIANG C N, DING X Z, BAO P J, et al. Cloning and sequence analysis of yak MSTN gene[J]. Acta Agriculturae Boreali-Sinica , 2013, 28(1):76-81. (in Chinese with English abstract)
[13] 刘丽霞,张丽,赵生国,等.八眉猪DQA基因编码区多态性及生物信息学分析[J].华北农学报,2015,30(1):103-108.
LIU L X, ZHANG L, ZHAO S G, et al. Cloning and bioinformatics analysis on coding regions of DQA gene in Bamei pigs[J]. Acta Agriculturae Boreali-Sinica , 2015, 30(1):103-108. (in Chinese with English abstract)
[14] 盘道兴,王振,杨茂林,等.江口萝卜猪FABPs主要家族基因SNPs筛选及生物信息学分析[J].基因组学与应用生物学, 2015(1):47-52.
PAN D X, WANG Z, YANG M L, et al.SNP screening and bioinformatics analysis of the main family genes of FABPs in Jiangkou Luobo pig[J]. Genomics and Applied Biology , 2015(1):47-52. (in Chinese with English abstract)
[15] 吴祖建,高芳銮,沈建国.生物信息学分析实践[M].北京:科学出版社,2012:93.
[16] 徐飞,成述儒,罗玉柱.绵羊 DRB 1基因生物信息学分析[J].生物技术通报,2011,27(1):113-118.
XU F, CHENG S R,LUO Y Z. Bioinformatics analysis of sheep DRB 1 gene[J]. Biotechnology Bulletin , 2011, 27(1):113-118. (in Chinese with English abstract)
[17] 罗轶.鸡 FATP 1基因cDNA的克隆、组织表达及其生物信息学分析[D].成都:四川农业大学,2008.
LUO Y. cDNA cloning, tissue expression and bioinformatic analysis of fatty acid transport Protrin 1 in chicken[D].Chengdu: Sichuan Agricultural University,2008. (in Chinese with English abstract)
[18] 蒋彦.基础生物信息学及应用[M].北京:清华大学出版社,2003:150-160.
[19] SKOLNICK J, FETROW J S. From genes to protein structure and function: novel applications of computational approaches in the genomic era[J]. Trends in Biotechnology , 2000, 18(1):34-39.
[20] 高峰,邢沈阳,于海滨,等.牛 MSTN 基因克隆及编码区E1-224-A>C定点突变前后生物信息学对比分析[J].中国兽医学报, 2014, 34(5):825-830.
GAO F,XING S Y,YU H B, et al. Cloning of bovine MSTN gene and bioinformatics comparative analysis between coding region with E1-224-A>C mutation site and normal type[J]. Chinese Journal of Veterinary Science , 2014, 34(5):825-830. (in Chinese with English abstract)
[21] 孙涛.基因突变对蛋白质影响的几种不同情况[J].中学生物教学, 2011(7):41-42.
SUN T. Several mutations of the protein on the different circumstances[J]. Middle School Biology Teaching , 2011(7):41-42. (in Chinese)
[22] DUNNER S, MIRANDA M E, AMIGUES Y, et al. Haplotype diversity of the myostatin gene among beef cattle breeds[J]. Genetics Selection Evolution , 2003, 35(1):103-118.
[23] GROBET L, PONCELET D, ROYO L J, et al. Molecular definition of an allelic series of mutations disrupting the myostatin function and causing double-muscling in cattle[J]. Mammalian Genome Official Journal of the International Mammalian Genome Society , 1998, 9(3):210-213.
[24] 冀德君,常洪,常春芳,等.中国牛属4个物种 MSTN 基因的遗传变异研究[J].畜牧兽医学报, 2008, 39(6):701-704.
JI D J,CHANG H,CHANG C F, et al. Genetic variation of myostatin gene in 4 bos species in China[J]. Acta Veterinaria et Zootechnica Sinica , 2008, 39(6):701-704. (in Chinese with English abstract)
[25] 贾浩,张小白,宋晓峰. 人类胞内蛋白半衰期与其亚细胞定位的相关性研究[J]. 计算机与应用化学,2011,28(4):411-414.
JIA H, ZHANG X B, SONG X F. Relationship between intracellular protein half-life and subcellular localization in human cells[J]. Computer and Applied Chemistry ,2011, 28(4):411-414. (in Chinese with English abstract)
[26] GURUPRASAD K,REDDY B,PANDIT M. Correlation between stability of a protein and its dipeptide composition: a novel approach for predicting in vivo stability of a protein from its primary sequence[J]. Protein Engineering ,1990,4(2):155-161.
[27] 郑海军,朱荣,葛春蕾,等. 人白细胞介素-29的生物信息学分析[J]. 中国生物制品学杂志,2013,26(2):209-212, 217.
ZHENG H J, ZHU R, GE C L, et al. Bioinformatics of human interleukin-29[J]. Chinese Journal of Biologicals , 2013,26(2):209-212, 217. (in Chinese with English abstract)

Cloning and bioinformatics analysis of MSTN gene of Tianzhu white yak

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHAO Xiuping, WANG Shuang, YAN Xingyi, DUAN Qiang, ZHANG Shuai, CHEN Yongsheng, LI Guorui. Expression, purification and bioinformatics analysis of Magnaporthe oryzae MGG-01005 [J]. Acta Agriculturae Zhejiangensis, 2021, 33(3): 470-478.
[2]	HE Jiaqi, ZHAI Ying, ZHANG Jun, QIU Shuang, LI Mingyang, ZHAO Yan, ZHANG Meijuan, MA Tianyi. Cloning and expression analysis of GmDof1.5 in soybean under abiotic stress [J]. Acta Agriculturae Zhejiangensis, 2021, 33(1): 1-7.
[3]	WANG Weike, LU Na, YAN Jing, SONG Jiling, YUAN Weidong, ZHOU Zufa. Cloning and expression analysis of PpSAMS gene of Pleurotus pulmonarius [J]. Acta Agriculturae Zhejiangensis, 2021, 33(1): 62-68.
[4]	LIANG Liqin, YANG Rui, GAO Gang. Bioinformatics analysis of StUOXs gene family in potato [J]. , 2020, 32(9): 1523-1532.
[5]	DU Yanbin, ZHANG Gangchen, WANG Yuxin, LIU Baobao, GONG Shenglong, DONG Xiao, WANG Yang. Cloning and bioinformatic analysis of rpoE gene of Streptococcus suis [J]. , 2020, 32(7): 1149-1154.
[6]	WU Jia, CHEN Lang, JIANG Tao, HUANG Guoming, LI Zhuo, LI Yaodong, ZHANG Li, LIU Lixia. Genetic polymorphism screening of CSF3 gene in dairy cow and its bioinformatics analysis [J]. , 2020, 32(6): 986-993.
[7]	WANG Yuanhong, XING Xue, LI Chuanfeng, ZHU Jie, WANG Yong, LIU Guangqing. Bioinformatics analysis and prokaryotic expression of FIPV AH1905 strain N gene [J]. , 2020, 32(3): 406-414.
[8]	WANG Xiaopeng, ZHAO Liang, LIU Zimin, BAI Caixia, YANG Kankan, ZHANG Da, SUN Pei, JIANG Shudong, LI Yongdong, WANG Yong. Prokaryotic expression and bioinformatics analysis of PPV7 Cap gene [J]. , 2020, 32(2): 200-209.
[9]	TAN Guanghui, QIN Yuanyu, LI Jiezhang, WU Lei, YUE Yong, ZHANG Yiyu. Single nucleotide polymorphism screening and bioinformatics analysis of ATF4 gene in Cherry Valley ducks [J]. , 2020, 32(2): 218-225.
[10]	QIN Ling, ZHANG Xin, RONG Chunxiao, MO Chuanyuan, FAN Lu, YAN Jie, MENG Ying, ZHANG Manrang. Identification and expression analysis of polyamine oxidase (PAO) gene family in apple [J]. , 2020, 32(2): 262-273.
[11]	ZHANG Haojie, LIU Mei, LI Chunyan, HE Ran, LAN Jingchao, LUO Li, GU Xiaobin, XIE Yue, YANG Guangyou. Prokaryotic expression and immunofluorescence localization of nucleoside diphosphate kinase gene from Dirofilaria immitis [J]. , 2019, 31(9): 1453-1460.
[12]	LIU Jialin, LIU Shili, JIANG Wenping, CHENG Shun, CHI Meili, ZHENG Jianbo, JIA Yongyi, ZHAO Jinliang, YIN Shaowu, GU Zhimin. Cloning and bioinformatics analysis of GH gene and its flanking region in Odontobutis potamophila [J]. , 2019, 31(9): 1461-1470.
[13]	YUAN Xiaolong, LI Yunqin, WANG Yi. Cloning and expression of three chalcone synthase-like genes from Paeonia delavayi [J]. , 2019, 31(9): 1478-1484.
[14]	PENG Shuai, CHEN Lang, ZHENG Tianyu, LU Huining, ZHANG Li, LIU Lixia. SNP site screening and bioinformatics analysis of TLR2 gene in Datong yak [J]. , 2019, 31(8): 1280-1285.
[15]	LIU Zhengkui, WU Yuan, CHEN Lin, WANG Lei, MU Hongye, ZHU Xuhang, WANG Xiaodu. Prokaryotic expression and bioinformatics analysis of Nsp5 gene of porcine epidemic diarrhea virus [J]. , 2019, 31(4): 532-538.