Analysis on phenotypic traits of red claw crayfish (Cherax quadricarinatus von Martens) in different growth stages

doi:10.3969/j.issn.1004-1524.2020.12.06

Acta Agriculturae Zhejiangensis ›› 2020, Vol. 32 ›› Issue (12): 2154-2161.DOI: 10.3969/j.issn.1004-1524.2020.12.06

• Animal Science • Previous Articles Next Articles

Analysis on phenotypic traits of red claw crayfish (Cherax quadricarinatus von Martens) in different growth stages

CHEN Honglin¹(), QIN Gaochan², LOU Bao¹^,^*(), QIAN Haojie³, YAO Zhenhai⁴

1. Institute of Hydrobiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
2. School of Fishery, Zhejiang Ocean University, Zhoushan 316022, China
3. Honghai Culture Co., Ltd., Haining 314400, China
4. Haining Aquaculture Technology Service Station of Zhejiang Province, Haining 314400, China

Received:2020-06-16 Online:2020-12-25 Published:2020-12-25
Contact: LOU Bao

Abstract

Abstract:

In order to study the phenotypic characteristics of red claw crayfish (Cherax quadricarinatus von Martens) in different growth stages, nine phenotypic traits including body weight (Y₁), hepatopancreas weight (Y₂), full-length (X₁), cephalothorax length (X₂), cephalothorax width (X₃), total abdominal segment length (X₄), first abdominal segment length (X₅), first abdominal segment width (X₆), propodite length (X₇) in three growth stages of 3 months, 4.5 months and 6 months were measured and analyzed. The differences of the phenotypic traits in the three stages were compared, and the correlations of the phenotypic traits in different growth stages were analyzed. Multiple regression equations of phenotypic traits to body weight of male and female red claw crayfish populations in different growth stages were constructed to measure the relevance of phenotypic traits and body weight. The results showed that the proportion of cephalothorax or abdominal segments to full-length remained the same in juvenile and adult stage, while the width index increased significantly (P<0.05) at 6 months of age. With the growth of red claw crayfish, the correlations among the majority of the traits decreased, and the differences in phenotypic characteristics between male and female populations gradually increased. The regression equation in 3 months of age was Y₁=-31.33+0.73X₂+0.91X₃. The regression equation in 4.5 months of age was Y₁=-65.25+1.29X₃+0.89X₄+1.46X₆ for female and Y₁=-69.67+1.17X₃+0.40X₇+1.77X₆+0.59X₄ for male, respectively. The regression equation in 6 months of age was Y₁=-79.77+1.01X₁-1.89X₆+1.15X₇ for female and Y₁=-98.02+0.72X₁+1.22X₇ for male, respectively. These results could provide theoretical basis for the selective breeding of red claw crayfish.

Key words: red claw crayfish, phenotypic characters, sexual dimorphism, correlation coefficient, regression analysis

CLC Number:

S966.12

CHEN Honglin, QIN Gaochan, LOU Bao, QIAN Haojie, YAO Zhenhai. Analysis on phenotypic traits of red claw crayfish (Cherax quadricarinatus von Martens) in different growth stages[J]. Acta Agriculturae Zhejiangensis, 2020, 32(12): 2154-2161.

Figures/Tables 6

References 25

[1]	MAC LOUGHLIN C, CANOSA I S, SILVEYRA G R, et al. Effects of atrazine on growth and sex differentiation, in juveniles of the freshwater crayfish Cherax quadricarinatus[J]. Ecotoxicology and Environmental Safety, 2016,131:96-103. URL PMID
[2]	ROUSE D B, AUSTIN C M, MEDLEY P B. Progress toward profits?: information on the Australian crayfish[J]. Aquaculture Magazine, 1991,17(3):46-56.
[3]	WICKINS J F, O’C LEE D. Crustacean farming, ranching and culture[J]. Aquaculture Research, 2003,34(3):269-270. DOI URL
[4]	TIERNEY L J, WILD C H, FURSE J M. Total incombustible (mineral) content of Cherax quadricarinatus differs between feral populations in Central-Eastern Australia[J]. PeerJ, 2019,7:e6351. URL PMID
[5]	王广军, 孙悦, 郁二蒙, 等. 澳洲淡水龙虾与克氏原螯虾肌肉营养成分分析与品质评价[J]. 动物营养学报, 2019,31(9):4339-4348.
	WANG G J, SUN Y, YU E M, et al. Analysis and quality evaluation of nutrient components in muscle of Cherax quadricarinatus and Procambarus clarkii[J]. Chinese Journal of Animal Nutrition, 2019,31(9):4339-4348.(in Chinese with English abstract)
[6]	顾志敏, 许谷星, 黄鲜明, 等. 红螯螯虾的室内人工育苗[J]. 水产学报, 2003,27(1):32-37.
	GU Z M, XU G X, HUANG X M, et al. Indoor artificial breeding and juvenile nursing of Cherax quadricarinatus[J]. Journal of Fisheries of China, 2003,27(1):32-37.(in Chinese with English abstract)
[7]	CORTÉS-JACINTO E, VILLARREAL-COLMENARES H, CIVERA-CERECEDO R, et al. Effect of dietary protein level on growth and survival of juvenile freshwater crayfish Cherax quadricarinatus(Decapoda: Parastacidae)[J]. Aquaculture Nutrition, 2003,9(4):207-213. DOI URL
[8]	GAO M L, LI F, XU L M, et al. White spot syndrome virus strains of different virulence induce distinct immune response in Cherax quadricarinatus[J]. Fish & Shellfish Immunology, 2014,39(1):17-23. DOI URL PMID
[9]	LIU H P, CHEN R Y, ZHANG Q X, et al. Differential gene expression profile from haematopoietic tissue stem cells of red claw crayfish, Cherax quadricarinatus, in response to WSSV infection[J]. Developmental and Comparative Immunology, 2011,35(7):716-724. DOI URL PMID
[10]	WANG D L, ZUO D, WANG L M, et al. Effects of white spot syndrome virus infection on immuno-enzyme activities and ultrastructure in gills of Cherax quadricarinatus[J]. Fish & Shellfish Immunology, 2012,32(5):645-650. DOI URL PMID
[11]	DUAN H, JIN S J, ZHANG Y, et al. Granulocytes of the red claw crayfish Cherax quadricarinatus can endocytose beads, E. coli and WSSV, but in different ways[J]. Developmental & Comparative Immunology, 2014,46(2):186-193. DOI URL PMID
[12]	BRYLAWSKI B J, MILLER T J. Temperature-dependent growth of the blue crab (Callinectes sapidus): a molt process approach[J]. Canadian Journal of Fisheries and Aquatic Sciences, 2006,63(6):1298-1308. DOI URL
[13]	MANOR R, AFLALO E D, SEGALL C, et al. Androgenic gland implantation promotes growth and inhibits vitellogenesis in Cherax quadricarinatus females held in individual compartments[J]. Invertebrate Reproduction & Development, 2004,45(2):151-159.
[14]	JONES C M. Production of juvenile redclaw crayfish, Cherax quadricarinatus(Von Martens) (Decapoda, Parastacidae) I: development of hatchery and nursery procedures[J]. Aquaculture, 1995,138(1/2/3/4):221-238. DOI URL
[15]	CURTIS M C, JONES C M. Observations on monosex culture of redclaw crayfish Cherax quadricarinatus von Martens (Decapoda: Parastacidae) in earthen ponds[J]. Journal of the World Aquaculture Society, 1995,26(2):154-159. DOI URL
[16]	费志良, 宋胜磊, 唐建清, 等. 克氏原螯虾含肉率及蜕皮周期中微量元素分析[J]. 水产科学, 2005,24(10):8-11.
	FEI Z L, SONG S L, TANG J Q, et al. Dressed crayfish and microelement levels in Procambarus clarkii during ecdysis[J]. Fisheries Science, 2005,24(10):8-11.(in Chinese with English abstract)
[17]	NISKANEN H, MANNONEN A, JUSSILA J. Variation in pleonite width in wild noble crayfish, Astacus astacus, females in relation to glair gland development[J]. Freshwater Crayfish, 1996 (11):378-383.
[18]	HOLLAND D L. Lipid reserves and energy metabolism in the larvae of benthic marine invertebrates[M]//SARGENT D C, MALINS J R. Biochemical and biophysical perspectives in marine biology. London: Academic Press, 1978: 85-123.
[19]	COCCIA E, DE LISA E, DI CRISTO C, et al. Effects of estradiol and progesterone on the reproduction of the freshwater crayfish Cherax albidus[J]. The Biological Bulletin, 2010,218(1):36-47. URL PMID
[20]	VOGT G. Functional cytology of the hepatopancreas of decapod crustaceans[J]. Journal of Morphology, 2019,280(9):1405-1444. URL PMID
[21]	RODRÍGUEZ-GONZÁLEZ H, HERNÁNDEZ-LLAMAS A, VILLARREAL H, et al. Gonadal development and biochemical composition of female crayfish Cherax quadricarinatus(Decapoda: Parastacidae) in relation to the gonadosomatic index at first maturation[J]. Aquaculture, 2006,254(1/2/3/4):637-645. DOI URL
[22]	RODRÍGUEZ-GONZÁLEZ H, VILLARREAL H, GARCÍA-ULLOA M, et al. Dietary lipid requirements for optimal egg quality of redclaw crayfish, Cherax quadricarinatus[J]. Journal of the World Aquaculture Society, 2009,40(4):531-539. DOI URL
[23]	SAGI A, KHALAILA I, ABDU U, et al. A newly established ELISA showing the effect of the androgenic gland on secondary-vitellogenic-specific protein in the hemolymph of the crayfish Cherax quadricarinatus[J]. General and Comparative Endocrinology, 1999,115(1):37-45. URL PMID
[24]	ABDU U, YEHEZKEL G, SAGI A. Oocyte development and polypeptide dynamics during ovarian maturation in the red-claw crayfish Cherax quadricarinatus[J]. Invertebrate Reproduction & Development, 2000,37(1):75-83.
[25]	TSENG D Y, CHEN Y N, KOU G H, et al. Hepatopancreas is the extraovarian site of vitellogenin synjournal in black tiger shrimp, Penaeus monodon[J]. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 2001,129(4):909-917. DOI URL

性状 Trait	月龄 Month	平均值 Mean	变异系数 CV/%
Y₁/g	3	19.30	28.03
	4.5	40.86	21.39
	6	62.75	24.57
Y₂/g	3	1.32	0.29
	4.5	2.75	0.26
	6	4.30	0.24
X₁/mm	3	94.44	10.11
	4.5	122.65	8.10
	6	138.72	7.76
X₂/mm	3	45.67	9.81
	4.5	59.17	8.01
	6	67.66	8.20
X₃/mm	3	18.93	12.04
	4.5	24.57	8.22
	6	29.10	8.52
X₄/mm	3	32.93	9.75
	4.5	42.57	7.56
	6	48.14	7.19
X₅/mm	3	7.62	10.37
	4.5	9.79	8.07
	6	11.72	14.51
X₆/mm	3	18.13	9.93
	4.5	23.97	9.01
	6	27.99	9.90
X₇/mm	3	28.44	11.60
	4.5	39.32	12.03
	6	50.35	12.95

性状 Trait	月龄 Month	平均值 Mean	变异系数 CV/%
Y₁/g	3	19.30	28.03
	4.5	40.86	21.39
	6	62.75	24.57
Y₂/g	3	1.32	0.29
	4.5	2.75	0.26
	6	4.30	0.24
X₁/mm	3	94.44	10.11
	4.5	122.65	8.10
	6	138.72	7.76
X₂/mm	3	45.67	9.81
	4.5	59.17	8.01
	6	67.66	8.20
X₃/mm	3	18.93	12.04
	4.5	24.57	8.22
	6	29.10	8.52
X₄/mm	3	32.93	9.75
	4.5	42.57	7.56
	6	48.14	7.19
X₅/mm	3	7.62	10.37
	4.5	9.79	8.07
	6	11.72	14.51
X₆/mm	3	18.13	9.93
	4.5	23.97	9.01
	6	27.99	9.90
X₇/mm	3	28.44	11.60
	4.5	39.32	12.03
	6	50.35	12.95

生长时期	X₂/X₁	X₃/X₁	X₄/X₁	X₅/X₁	X₆/X₁	X₇/X₁	Y₂/Y₁
Growth stage
3月龄3 months	48.89±0.97 a	20.31±0.73 b	34.33±0.99 a	7.99±0.50 b	18.84±0.63 b	31.26±2.27 c	7.03±1.05 a
4.5月龄4.5 months	48.28±1.11 a	20.05±0.65 b	34.72±1.08 a	7.99±0.48 b	19.54±0.74 b	32.07±2.76 b	6.79±1.29 a
6月龄 6 months	48.77±1.54 a	21.00±1.20 a	34.74±1.30 a	8.47±1.21 a	20.20±1.55 a	36.21±2.80 a	6.98±1.20 a

生长时期	X₂/X₁	X₃/X₁	X₄/X₁	X₅/X₁	X₆/X₁	X₇/X₁	Y₂/Y₁
Growth stage
3月龄3 months	48.89±0.97 a	20.31±0.73 b	34.33±0.99 a	7.99±0.50 b	18.84±0.63 b	31.26±2.27 c	7.03±1.05 a
4.5月龄4.5 months	48.28±1.11 a	20.05±0.65 b	34.72±1.08 a	7.99±0.48 b	19.54±0.74 b	32.07±2.76 b	6.79±1.29 a
6月龄 6 months	48.77±1.54 a	21.00±1.20 a	34.74±1.30 a	8.47±1.21 a	20.20±1.55 a	36.21±2.80 a	6.98±1.20 a

性状Trait	性别Sex	3月龄3 months	4.5月龄4.5 months	6月龄6 months
Y₁	雌Female	24.44±7.07	41.04±8.34	57.93±17.24^*
	雄Male	24.77±6.70	40.44±9.31	65.45±13.92
Y₂	雌Female	1.72±0.61	2.96±0.72^**	4.13±0.99
	雄Male	1.74±0.50	2.53±0.65	4.4±1.03
X₁	雌Female	103.02±11.47	123.99±9.44	137.94±14.09
	雄Male	103.05±10.63	121.34±10.41	139.42±8.41
X₂	雌Female	50.24±5.82	59.48±4.66	66.81±6.30
	雄Male	50.75±5.78	58.79±4.90	68.42±4.62
X₃	雌Female	20.87±2.62	24.92±1.94	29.25±3.21
	雄Male	21.17±2.67	24.19±2.10	29.04±2.03
X₄	雌Female	35.44±3.53	42.94±3.04	49.05±4.43
	雄Male	35.03±3.33	42.06±3.39	47.66±2.74
X₅	雌Female	8.21±1.00	9.81±0.81	12.66±2.37^**
	雄Male	8.24±0.88	9.73±0.79	11.19±0.86
X₆	雌Female	19.49±2.02	24.65±2.20^**	29.96±3.40^**
	雄Male	19.07±1.74	23.23±1.93	26.92±1.57
X₇	雌Female	31.92±4.60	38.58±4.74	48.16±6.92^*
	雄Male	32.95±4.94	40.07±4.78	51.60±6.08

Analysis on phenotypic traits of red claw crayfish (Cherax quadricarinatus von Martens) in different growth stages

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性状Trait	Y₁	Y₂	X₁	X₂	X₃	X₄	X₅	X₆	X₇
Y₁	1.00	0.81	0.98^**	0.98^**	0.98^**	0.97^**	0.84	0.97^**	0.54
Y₂	0.79	1.00	0.79	0.79	0.82	0.72	0.63	0.77	0.47
X₁	0.96^**	0.70	1.00	0.98^**	0.95^**	0.97^**	0.84	0.97^**	0.46
X₂	0.95^**	0.68	0.97^**	1.00	0.96^**	0.97^**	0.84	0.98^**	0.49
X₃	0.94^**	0.70	0.94^**	0.94^**	1.00	0.94^**	0.81	0.95^**	0.52
X₄	0.96^**	0.79	0.94^**	0.92^**	0.91^**	1.00	0.87^**	0.98^**	0.44
X₅	0.82	0.71	0.81	0.82	0.78	0.86^**	1.00	0.87^**	0.39
X₆	0.94^**	0.80	0.94^**	0.91^**	0.90^**	0.95^**	0.84	1.00	0.44
X₇	0.77	0.79	0.81	0.76	0.70	0.68	0.70	0.52	1.00

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