Effects of oral Panax notoginseng saponins on immunity of large yellow croaker (Pseudosciaena crocea)

doi:10.3969/j.issn.1004-1524.2021.04.06

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (4): 610-617.DOI: 10.3969/j.issn.1004-1524.2021.04.06

• Animal Science • Previous Articles Next Articles

Effects of oral Panax notoginseng saponins on immunity of large yellow croaker (Pseudosciaena crocea)

CHEN Xiuxia(), CHI Hongshu, XU Binfu, XIE Anhua, FANG Donglan, GONG Hui^*()

Biotechnology Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China

Received:2020-11-10 Online:2021-04-25 Published:2021-04-25
Contact: GONG Hui

Abstract

Abstract:

In this study, large yellow croaker (Pseudosciaena crocea) were fed with diets containing 0,10,50,250 mg·kg^-1 Panax notoginseng saponins (PNS) for 14 d. To evaluate the effects of PNS on the immunity of large yellow croaker, the expression of some immune-related genes in the liver, spleen and head kidney tissues, the activities of some serum immune factors and the resistance against Pseudomonas plecoglossicida were determined. Results showed that the activities of serum superoxide dismutase (SOD), lysozyme (LZM), acp acid phosphatase (ACP) and alkaline phosphatase (AKP) in the experimental groups were not significantly different from the control group; the expression of Mx in spleen of fish fed with 250 mg·kg ^-1 PNS was significantly higher than that of control; the expression of Mx in spleen of fish fed with 50 mg·kg ^-1 PNS and in head-kidney of fish fed with 10 mg·kg^-1 PNS were higher than that of control, but not significantly; the expression of TNF-α in all the exprimental groups were lower than that of control, but not significantly; the expression of IL-10 and MHCⅡ-α in all the exprimental groups were not significantly different from the control. The mortalities of the exprimental fish challenged with P. plecoglossicida were not significantly different from the control. It was indicated that feeding diets containing 10-250 mg·kg ^-1 PNS for 14 d did not improve the immunity of large yellow croaker

Key words: Panax notoginseng saponins, large yellow croaker (Pseudosciaena crocea), immunity

CLC Number:

S965.322

CHEN Xiuxia, CHI Hongshu, XU Binfu, XIE Anhua, FANG Donglan, GONG Hui. Effects of oral Panax notoginseng saponins on immunity of large yellow croaker (Pseudosciaena crocea)[J]. Acta Agriculturae Zhejiangensis, 2021, 33(4): 610-617.

Figures/Tables 6

Table 1 Primers used for real-time PCR

基因 Genes	引物核酸序列 Nucleic acid sequence of primers(5'-3')
Mx	上游引物 Forward primer: AGTTTTCCTCTTATTCGCCTGATTTTT
	下游引物 Reverse primer: TAAAAAGAACAAGTCAGCCAGGTGC
MHCⅡ-α	上游引物 Forward primer: TCAGCTGCACTCGTTCC
	下游引物 Reverse primer: CCAGCATCAACGTTCCTC
TNF-α	上游引物 Forward primer: TAAGAACACCTACGAGACCATCCTGC
	下游引物 Reverse primer: CTCTTCTCCATCACCGTCTTCAGTTT
IL-10	上游引物 Forward primer: CATCAACAGCATTTTAGATTTTTACC
	下游引物 Reverse primer: CTGAGTGTAAGTGGAGTTTAGGTTTG
β-actin	上游引物 Forward primer: GACCTGACAGACTACCTCATG
	下游引物 Reverse primer: AGTTGAAGGTGGTCTCGTGGA

Fig.1 Activities of serum nonspecific immune parameters of large yellow croaker Different lowercase letters above the columns represent statistically significant (P<0.05) differences among treatments. The treatment group 0, 1, 2, 3 represented the treatment with diets containing 0, 10, 50, 250 mg·kg-1 PNS. The same as below.

Fig.2 Relative expression levels of immune related factors in liver

Fig.3 Relative expression levels of immune related factors in spleen

Fig.4 Relative expression levels of immune related factors in head-kidney

Fig.5 Mortality per day of large yellow croaker challenged with Pseudomonas plecoglossicida

References 17

[1]	谭亮, 汤秋凯, 王守章, 等. 三七皂苷R1药理作用的研究进展[J]. 中国药理学通报, 2018,34(5):604-607.
	TAN L, TANG Q K, WANG S Z, et al. Research progress on pharmacological action of notoginsenoside R1[J]. Chinese Pharmacological Bulletin, 2018,34(5):604-607.(in Chinese with English abstract)
[2]	方鹏飞, 常丽霞, 宋渊. 三七总皂苷临床应用研究进展[J]. 中医药学报, 2016,44(3):120-123.
	FANG P F, CHANG L X, SONG Y. Progress in clinical application of Panax notoginseng total saponins[J]. Acta Chinese Medicine and Pharmacology, 2016,44(3):120-123.(in Chinese)
[3]	张海燕. 5种皂甙对小鼠免疫功能的影响对比试验[J]. 山西农业科学, 2013,41(5):458-459.
	ZHANG H Y. Effect contrast experiment of 5 saponins on mice immunity[J]. Journal of Shanxi Agricultural Sciences, 2013,41(5):458-459.(in Chinese with English abstract)
[4]	赵鹏, 李彬, 何为涛, 等. 三七皂甙对小鼠免疫功能影响的实验研究[J]. 中国热带医学, 2004(4):522-524.
	ZHAO P, LI B, HE W T, et al. Experimental study on the effect of Panax notoginoside on enhancment of mice’s immune function[J]. China Tropical Medicine, 2004(4):522-524.(in Chinese with English abstract)
[5]	杨志刚, 忻晨, 郑剑伟, 等. 三七总皂苷在罗非鱼饲料中的应用试验[J]. 饲料研究, 2008(10):51-52.
	YANG Z G, XIN C, ZHENG J W, et al. Application of Panax notoginseng saponins in tilapia feed[J]. Feed Research, 2008(10):51-52.(in Chinese)
[6]	杨志刚, 陈志刚, 刘志伟, 等. 三七总皂苷对异育银鲫非特异免疫及抗氧化性能的影响[J]. 饲料研究, 2010(8):66-68.
	YANG Z G, CHEN Z G, LIU Z W, et al. Effects of Panax notoginseng saponins on the nonspecific immunity and oxidation resistance in Carassius auratus gibelio[J]. Feed Research, 2010(8):66-68.(in Chinese)
[7]	CHEN X X, CHI H S, XU B F, et al. Oral bovine serum albumin immune-stimulating complexes improve the immune responses and resistance of large yellow croaker (Pseudosciaena crocea, Richardson, 1846) against Vibrio alginolyticus[J]. Aquaculture Research, 2019,50(10):2802-2809.
[8]	LIVAK K J, SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2^-ΔΔCT method[J]. Methods, 2001,25(4):402-408. DOI URL PMID
[9]	SUN H X, PAN H J, PAN Y J. Haemolytic activities and immunologic adjuvant effect of Panax notoginseng saponins[J]. Acta Pharmacologica Sinica, 2003,24(11):1150-1154. URL PMID
[10]	周小玲, 李永伟, 李逢春, 等. 三七总皂甙对大鼠免疫功能影响的实验研究[J]. 广西医科大学学报, 2001,18(3):360-361.
	ZHOU X L, LI Y W, LI F C, et al. The experimental study of Panax natoginseng saponins on immune functions of rats[J]. Journal of Guangxi Medical University, 2001,18(3):360-361.(in Chinese with English abstract)
[11]	秦枫, 陈玉勇, 冒美丽, 等. 三七皂苷PNS-5的溶血性及生物免疫活性研究[J]. 中国医药导报, 2008,29(5):10-11.
	QIN F, CHEN Y Y, MAO M L, et al. Study on hemolytic characteristics and immunocompetence of biology of PNS-5[J]. China Medical Herald, 2008,29(5):10-11.(in Chinese with English abstract)
[12]	顾国嵘, 黄培志, 费国强, 等. 三七总皂甙预处理对肿瘤坏死因子表达的影响[J]. 中国临床医学, 2005,12(4):721-723.
	GU G R, HUANG P Z, FEI G Q, et al. The effect of preconditioning with Panax notoginseng saponins on the expression of tumor necrosis factor-Α[J]. Clinical Medical Journal of China, 2005,12(4):721-723.(in Chinese with English abstract)
[13]	姚茹冰, 赵智明, 蔡辉. 活血化瘀中药三七抗炎及免疫调节作用研究进展[J]. 中西医结合心脑血管病杂志, 2009,7(6):720-721.
	YAO R B, ZHAO Z M, CAI H. Research progress on anti-inflammatory and immunomodulatory effects of Panax notoginseng, a traditional Chinese medicine for promoting blood circulation and removing blood stasis[J]. Chinese Journal of Integrative Medicine on Cardio-/Cerebrovascular Disease, 2009,7(6):720-721.(in Chinese)
[14]	周兴宏, 刘晓瑜, 温子云, 等. 三七皂苷R1上调miR-146a水平和减轻ApoE-/-小鼠肝脏炎症损伤的研究[J]. 中药新药与临床药理, 2016,27(3):317-321.
	ZHOU X H, LIU X Y, WEN Z Y, et al. Study of notoginsenoside R1 relieving liver inflammatory injury by up-regulating miR-146a level in ApoE-/- mice[J]. Traditional Chinese Drug Research and Clinical Pharmacology, 2016,27(3):317-321.(in Chinese with English abstract)
[15]	BEAMER C A, SHEPHERD D M. Inhibition of TLR ligand-and interferon gamma-induced murine microglial activation by Panax notoginseng[J]. Journal of Neuroimmune Pharmacology, 2012,7(2):465-476. URL PMID
[16]	HU Y Y, HE K W, WANG X M. Role of Chinese herbal medicinal ingredients in secretion of cytokines by PCV2-induced endothelial cells[J]. Journal of Immunotoxicology, 2016,13(2):141-147. DOI URL PMID
[17]	陈秀霞, 郑在予, 黄河, 等. 三七总皂甙对大黄鱼肾细胞生长及部分免疫相关基因表达的影响[J]. 福建畜牧兽医, 2017,39(6):7-9.
	CHEN X X, ZHENG Z Y, HUANG H, et al. The effects of Panax notoginseng saponins on the growth and the immune related genes expression of large yellow croaker kidney cells[J]. Fujian Journal of Animal Husbandry and Veterinary Medicine, 2017,39(6):7-9.(in Chinese with English abstract)

Effects of oral Panax notoginseng saponins on immunity of large yellow croaker (Pseudosciaena crocea)

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