Effects of oral Panax notoginseng saponins on immunity of large yellow croaker (Pseudosciaena crocea)

doi:10.3969/j.issn.1004-1524.2021.04.06

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (4): 610-617.DOI: 10.3969/j.issn.1004-1524.2021.04.06

• Animal Science • Previous Articles Next Articles

Effects of oral Panax notoginseng saponins on immunity of large yellow croaker (Pseudosciaena crocea)

CHEN Xiuxia(), CHI Hongshu, XU Binfu, XIE Anhua, FANG Donglan, GONG Hui^*()

Biotechnology Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China

Received:2020-11-10 Online:2021-04-25 Published:2021-04-25
Contact: GONG Hui

Abstract

Abstract:

In this study, large yellow croaker (Pseudosciaena crocea) were fed with diets containing 0,10,50,250 mg·kg^-1 Panax notoginseng saponins (PNS) for 14 d. To evaluate the effects of PNS on the immunity of large yellow croaker, the expression of some immune-related genes in the liver, spleen and head kidney tissues, the activities of some serum immune factors and the resistance against Pseudomonas plecoglossicida were determined. Results showed that the activities of serum superoxide dismutase (SOD), lysozyme (LZM), acp acid phosphatase (ACP) and alkaline phosphatase (AKP) in the experimental groups were not significantly different from the control group; the expression of Mx in spleen of fish fed with 250 mg·kg ^-1 PNS was significantly higher than that of control; the expression of Mx in spleen of fish fed with 50 mg·kg ^-1 PNS and in head-kidney of fish fed with 10 mg·kg^-1 PNS were higher than that of control, but not significantly; the expression of TNF-α in all the exprimental groups were lower than that of control, but not significantly; the expression of IL-10 and MHCⅡ-α in all the exprimental groups were not significantly different from the control. The mortalities of the exprimental fish challenged with P. plecoglossicida were not significantly different from the control. It was indicated that feeding diets containing 10-250 mg·kg ^-1 PNS for 14 d did not improve the immunity of large yellow croaker

Key words: Panax notoginseng saponins, large yellow croaker (Pseudosciaena crocea), immunity

CLC Number:

S965.322

CHEN Xiuxia, CHI Hongshu, XU Binfu, XIE Anhua, FANG Donglan, GONG Hui. Effects of oral Panax notoginseng saponins on immunity of large yellow croaker (Pseudosciaena crocea)[J]. Acta Agriculturae Zhejiangensis, 2021, 33(4): 610-617.

Figures/Tables 6

Table 1 Primers used for real-time PCR

基因 Genes	引物核酸序列 Nucleic acid sequence of primers(5'-3')
Mx	上游引物 Forward primer: AGTTTTCCTCTTATTCGCCTGATTTTT
	下游引物 Reverse primer: TAAAAAGAACAAGTCAGCCAGGTGC
MHCⅡ-α	上游引物 Forward primer: TCAGCTGCACTCGTTCC
	下游引物 Reverse primer: CCAGCATCAACGTTCCTC
TNF-α	上游引物 Forward primer: TAAGAACACCTACGAGACCATCCTGC
	下游引物 Reverse primer: CTCTTCTCCATCACCGTCTTCAGTTT
IL-10	上游引物 Forward primer: CATCAACAGCATTTTAGATTTTTACC
	下游引物 Reverse primer: CTGAGTGTAAGTGGAGTTTAGGTTTG
β-actin	上游引物 Forward primer: GACCTGACAGACTACCTCATG
	下游引物 Reverse primer: AGTTGAAGGTGGTCTCGTGGA

Fig.1 Activities of serum nonspecific immune parameters of large yellow croaker Different lowercase letters above the columns represent statistically significant (P<0.05) differences among treatments. The treatment group 0, 1, 2, 3 represented the treatment with diets containing 0, 10, 50, 250 mg·kg-1 PNS. The same as below.

Fig.2 Relative expression levels of immune related factors in liver

Fig.3 Relative expression levels of immune related factors in spleen

Fig.4 Relative expression levels of immune related factors in head-kidney

Fig.5 Mortality per day of large yellow croaker challenged with Pseudomonas plecoglossicida

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Effects of oral Panax notoginseng saponins on immunity of large yellow croaker (Pseudosciaena crocea)

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