Isolation and identification of fungi from mildewed feed corn and study on anti-mildew and antifungal effects of lactobacillin

doi:10.3969/j.issn.1004-1524.2021.07.14

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (7): 1283-1291.DOI: 10.3969/j.issn.1004-1524.2021.07.14

• Environmental Science • Previous Articles Next Articles

Isolation and identification of fungi from mildewed feed corn and study on anti-mildew and antifungal effects of lactobacillin

JIANG Yuhang¹^,²(), XIN Weigang¹^,², ZHANG Qili¹^,², DENG Xianyu¹^,², WANG Feng¹^,², LIN Lianbing¹^,²^,^*()

1. Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China
2. Engineering Research Center for Replacement Technology of Feed Antibiotics of Yunnan College, Kunming 650500, China

Received:2020-10-12 Online:2021-07-25 Published:2021-08-06
Contact: LIN Lianbing

Abstract

Abstract:

To clarify composition of fungi species in mildewed feed corn and explore antifungal effect of lactobacillin on mildewed fungi and the effect of delaying feed corn mildewing, in this study, mildew fungi were isolated from mildewed feeding corn by using traditional fungi culture method, and the taxonomic status of culturable fungi in mildewed feed corn was determined by sequencing of ITS amplicons. The inhibitory effect of lactobacillin on mildew fungi was evaluated using the Oxford Cup method, and crude lactobacillin with different proportions (10%, 20%, 30%) were set to explore mildew cycle and mildew degree of lactobacillin on delayed feeding corn mildew (caking, mildew spot, mildew taste, and mildew number were taken as observation indices). The results showed that diversity of fungi species in mildewed feeding corn was high, and five genera of fungus (Aspergillus, Penicillium, Mucor, Fusarium and Geotrichum) belonging to three phyla were screened. Lactobacillin had a good inhibitory effects on mildew fungi of various genera in mildewed feed corn, it had the best antifungal activity against Penicillium crustosum, the diameter of the inhibition circle could reach (16.54 ± 0.23) mm. The antifungal activity against Geotrichum candidum was the worst, only with the diameter of inhibition zone of (9.26±0.16) mm, but their diameter of inhibition zones were significantly (P <0.05) higher than that of the control group. Different proportions of lactobacillin crude extract were added to feeding corn, there was no obvious mildew phenomen within 10 days, and there was obvious difference in the degree of mildew between the treatment groups and the control group on the 30th day. In addition, with the increasing of proportion of added lactobacillin, the mildewing degree of feed corn gradually decreased, and the fungi colonies were only (9.28±0.59) × 10⁷ CFU·g^-1 in the treatment group with 30% addition proportion. In conclusion, there were rich species of fungi in moldy feed corn, and there were a variety of toxin-producing molds; Lactobacillin could effectively inhibit mildew fungi, slow down the cycle and degree of mildew in feed corn.

Key words: feed corn, mildew fungi, strain identification, lactobacillin, antibacterial activity

CLC Number:

S513

JIANG Yuhang, XIN Weigang, ZHANG Qili, DENG Xianyu, WANG Feng, LIN Lianbing. Isolation and identification of fungi from mildewed feed corn and study on anti-mildew and antifungal effects of lactobacillin[J]. Acta Agriculturae Zhejiangensis, 2021, 33(7): 1283-1291.

Figures/Tables 5

References 36

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门 Phylum	菌株 Strain	分子鉴定 Molecular identification	序列一致性 Sequence identity/%	GenBank 登录号 GenBank accession No.
子囊菌门Ascomycota	MR-1	黄曲霉Aspergillus flavus	100	MG818938
半知菌亚门Deuteromycotina	MR-4	米曲霉 Aspergillus oryzae	100	MK748312
	MR-6	黑曲霉 Aspergillus niger	100	MN509035
	MR-7	皮壳青霉 Penicillium crustosum	99	MG975627
	MR-10	拟轮枝镰孢菌 Fusarium verticillioides	100	MN429250
	MR-12	白地霉 Geotrichum candidum	99	KT828737
接合菌亚门Zygomycotina	MR-8	卷枝毛霉 Mucor circinelloides	99	MK559692

门 Phylum	菌株 Strain	分子鉴定 Molecular identification	序列一致性 Sequence identity/%	GenBank 登录号 GenBank accession No.
子囊菌门Ascomycota	MR-1	黄曲霉Aspergillus flavus	100	MG818938
半知菌亚门Deuteromycotina	MR-4	米曲霉 Aspergillus oryzae	100	MK748312
	MR-6	黑曲霉 Aspergillus niger	100	MN509035
	MR-7	皮壳青霉 Penicillium crustosum	99	MG975627
	MR-10	拟轮枝镰孢菌 Fusarium verticillioides	100	MN429250
	MR-12	白地霉 Geotrichum candidum	99	KT828737
接合菌亚门Zygomycotina	MR-8	卷枝毛霉 Mucor circinelloides	99	MK559692

菌株 Strains	抑菌圈直径 Inhibition zone diameter	对照组 Control group
MR-1	11.36±0.06^*	0.30±0.03
MR-4	12.24±0.18^*	0.16±0.06
MR-6	15.65±0.22^*	0.12±0.04
MR-7	16.54±0.23^*	0.31±0.10
MR-8	13.36±0.12^*	0.52±0.06
MR-10	14.45±0.13^*	0.23±0.01
MR-12	9.26±0.16^*	0.41±0.02

菌株 Strains	抑菌圈直径 Inhibition zone diameter	对照组 Control group
MR-1	11.36±0.06^*	0.30±0.03
MR-4	12.24±0.18^*	0.16±0.06
MR-6	15.65±0.22^*	0.12±0.04
MR-7	16.54±0.23^*	0.31±0.10
MR-8	13.36±0.12^*	0.52±0.06
MR-10	14.45±0.13^*	0.23±0.01
MR-12	9.26±0.16^*	0.41±0.02

t/d	处理组Treatment group			对照组Control group
t/d	10%	20%	30%	CA10%	CA20%		CA30%
5	—,—,—	—,—,—	—,—,—	—,—,—		—,—,+		—,*,+
10	—,—,—	—,—,—	—,—,—	—,*,+		〇,*,++		〇〇,**,++
15	〇,*,—	—,*,+	—,*,—	〇,*,+		〇〇,**,++		〇〇〇,***,+++
20	〇,*,++	〇,*,++	〇,*,+	〇,**,++		〇〇〇,***,+++		〇〇〇〇,****,+++
25	〇〇,*,++	〇,**,++	〇,**,+	〇〇,***,++		〇〇〇〇,****,+++		〇〇〇〇〇,*****,++++
30	〇〇,**,++	〇,**,++	〇,**,+	〇〇〇,***,+++		〇〇〇〇〇,*****,++++		〇〇〇〇〇,*****,++++

Isolation and identification of fungi from mildewed feed corn and study on anti-mildew and antifungal effects of lactobacillin

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