Residue dynamic and risk assessment of three fungicides in broccoli

doi:10.3969/j.issn.1004-1524.2021.07.15

Acta Agriculturae Zhejiangensis ›› 2021, Vol. 33 ›› Issue (7): 1292-1299.DOI: 10.3969/j.issn.1004-1524.2021.07.15

• Quality and Safety of Agricultural Products • Previous Articles Next Articles

Residue dynamic and risk assessment of three fungicides in broccoli

SUN Caixia¹(), OUYANG Zhizhou²^,³, LIU Yuhong¹, YU Guoguang¹

1. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products(SKLQSAP), Institute of Agro-Product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
2. School of Environment Science and Engineering, Tianjin University, Tianjin 300072, China
3. Environmental Science and Engineering School, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250000, China

Received:2020-07-26 Online:2021-07-25 Published:2021-08-06

Abstract

Abstract:

Pesticide residue is the key impact factor that affect vegetable quality and safety. In this article, broccoli was studied and the residue dynamic trial of three triazole pesticides including difenoconazole, tebuconazole and myclobutanil was carried out. The GC-MS method was established to detect triazole pesticides residue and dietary intake riskassessment of the three pesticides was carried out. The results showed that, according to the dietary intake data, the usage of difenoconazole and myclobutanil should be controlled and post-harvest interval should be longer than 28 days, application of tebuconazole in broccoli should be assessed further.

Key words: broccoli, pesticide residue, triazole fungicide, risk assessment, dietary intake, preharvest-interval

CLC Number:

S481.8

SUN Caixia, OUYANG Zhizhou, LIU Yuhong, YU Guoguang. Residue dynamic and risk assessment of three fungicides in broccoli[J]. Acta Agriculturae Zhejiangensis, 2021, 33(7): 1292-1299.

Figures/Tables 5

References 15

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农药 Pesticide	施用量Application amount		有效成分用量Active ingredient dosage
农药 Pesticide	1.0倍用药 1.0 time the pesticide	1.5倍用药 1.5 times the pesticide	1.0倍用药 1.0 time the pesticide	1.5倍用药 1.5 times the pesticide
苯醚甲环唑(10%水分散剂)	40.00	60.00	4.00	6.00
Difenoconazole (10% water dispersant)
戊唑醇(30%乳油) Tebuconazole (30% EC)	15.00	22.50	4.50	6.75
腈菌唑(25%乳油) Myclobutanil (25% EC)	40.00	60.00	10.00	15.00

农药 Pesticide	施用量Application amount		有效成分用量Active ingredient dosage
农药 Pesticide	1.0倍用药 1.0 time the pesticide	1.5倍用药 1.5 times the pesticide	1.0倍用药 1.0 time the pesticide	1.5倍用药 1.5 times the pesticide
苯醚甲环唑(10%水分散剂)	40.00	60.00	4.00	6.00
Difenoconazole (10% water dispersant)
戊唑醇(30%乳油) Tebuconazole (30% EC)	15.00	22.50	4.50	6.75
腈菌唑(25%乳油) Myclobutanil (25% EC)	40.00	60.00	10.00	15.00

农药 Pesticide	线性范围 Linear range/ (μg·L^-1)	线性方程 Linear equation	相关系数 Correlation coefficient	检出限 Detection limit/ (μg·L^-1)	定量限 Limit of quantitation/ (μg·L^-1)	RSD/% (n=7)
苯醚甲环唑Difenoconazole	5~100	y=427.52x-29.9	0.994 0	1.04	3.46	13.2
戊唑醇Tebuconazole	1~100	y=309.12x+27.5	0.995 4	0.24	0.79	11.4
腈菌唑Myclobutanil	1~100	y=643.24x-45.1	0.997 6	0.14	0.46	10.3

农药 Pesticide	线性范围 Linear range/ (μg·L^-1)	线性方程 Linear equation	相关系数 Correlation coefficient	检出限 Detection limit/ (μg·L^-1)	定量限 Limit of quantitation/ (μg·L^-1)	RSD/% (n=7)
苯醚甲环唑Difenoconazole	5~100	y=427.52x-29.9	0.994 0	1.04	3.46	13.2
戊唑醇Tebuconazole	1~100	y=309.12x+27.5	0.995 4	0.24	0.79	11.4
腈菌唑Myclobutanil	1~100	y=643.24x-45.1	0.997 6	0.14	0.46	10.3

农药 Pesticide	加标浓度 Spiked concentration/(μg·L^-1)	西兰花样品Broccoli sample(n=3)
农药 Pesticide	加标浓度 Spiked concentration/(μg·L^-1)	回收率Recovery rate/%	相对标准偏差RSD/%
苯醚甲环唑Difenoconazole	20	88.4	12.9
	50	117.8	2.8
	100	115.1	15.6
戊唑醇Tebuconazole	20	82.8	8.7
	50	109.8	14.9
	100	116.4	10.3
腈菌唑Myclobutanil	20	103.4	13.1
	50	107.6	10.3
	100	111.8	16.1

Residue dynamic and risk assessment of three fungicides in broccoli

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剂量 Dose	用药次数 Number of medications	栽培模式 Cultivation mode		苯醚甲环唑 Difenoconazole	戊唑醇 Tebuconazole	腈菌唑 Myclobutanil
1.0倍剂量 1.0 time the dose	1次用药 1 dose	露地 Open field	降解方程 Degradation equation	y=0.855+0.154x	y=0.872+0.172x	y=0.877+0.194x
			r	0.968	0.914	0.964
			T_1/2/d	4.50	4.03	3.29
		大棚 Greenhouse	降解方程 Degradation equation	y=0.810+0.150x	y=0.805+0.166x	y=0.839+0.16x
			r	0.973	0.951	0.934
			T_1/2/d	4.62	4.17	4.33
	2次用药 2 dose	露地 Open field	降解方程 Degradation equation	y=0.875+0.127x	y=0.836+0.094x	y=0.738+0.112x
			r	0.962	0.984	0.906
			T_1/2/d	5.45	7.37	6.18
		大棚 Greenhouse	降解方程 Degradation equation	y=0.766+0.122x	y=0.852+0.067x	y=0.821+0.101x
			r	0.947	0.978	0.969
			T_1/2/d	5.68	10.34	6.86
1.5倍剂量 1.5 times the dose	1次用药 1 dose	露地 Open field	降解方程 Degradation equation	y=0.522+0.136x	y=0.476+0.226x	y=0.539+0.166x
			r	0.934	0.925	0.942
			T_1/2/d	5.09	3.06	4.17
		大棚 Greenhouse	降解方程 Degradation equation	y=0.529+0.125x	y=0.519+0.196x	y=0.533+0.133x
			r	0.958	0.913	0.961
			T_1/2/d	4.58	3.53	5.21
	2次用药 2 dose	露地 Open field	降解方程 Degradation equation	y=0.571+0.11x	y=0.632+0.063x	y=0.558+0.078x
			r	0.901	0.924	0.949
			T_1/2/d	6.30	11	8.88
		大棚 Greenhouse	降解方程 Degradation equation	y=0.556+0.105x	y=0.566+0.059x	y=0.616+0.069x
			r	0.922	0.947	0.933
			T_1/2/d	6.60	11.74	10.04

剂量 Dose	安全间隔期 PHI/d	栽培模式 Cultivation mode	苯醚甲环唑Difenoconazole			戊唑醇Tebuconazole			腈菌唑Myclobutanil
剂量 Dose	安全间隔期 PHI/d	栽培模式 Cultivation mode	CRL/ (mg·kg^-1)	NEDI	RQ	CRL/ (mg·kg^-1)	NEDI	RQ	CRL/ (mg·kg^-1)	NEDI	RQ
1.0倍剂量	14	露地Open field	0.104	0.047 3	4.73	0.106	0.0482	1.61	0.142	0.064 6	2.15
1.0 times		大棚Greenhouse	0.114	0.051 8	5.18	0.132	0.0601	2.01	0.124	0.056 4	1.88
the dose	21	露地Open field	0.046	0.020 9	2.09	0.076	0.0345	1.15	0.085	0.038 6	1.28
		大棚Greenhouse	0.054	0.024 5	2.45	0.108	0.0491	1.638	0.069	0.031 4	1.04
	28	露地Open field	0.010	0.004 6	0.455	0.0280	0.0127	0.425	0.012	0.005 5	0.182
		大棚Greenhouse	0.011	0.005 0	0.500	0.071	0.0323	1.076	0.022	0.0100	0.333
1.5倍剂量	14	露地Open field	0.142	0.064 6	6.46	0.202	0.0919	3.06	0.186	0.0846	2.82
1.5 times		大棚Greenhouse	0.165	0.075 1	7.51	0.226	0.102 0	3.42	0.196	0.0891	2.97
the dose	21	露地Open field	0.106	0.048 2	4.82	0.176	0.080 0	2.67	0.136	0.0618	2.06
		大棚Greenhouse	0.102	0.046 4	4.64	0.208	0.0946	3.15	0.126	0.0573	1.91
	28 d	露地Open field	0.018	0.004 9	0.491	0.088	0.0400	1.33	0.062	0.0282	0.940
		大棚Greenhouse	0.022	0.0100	1.01	0.101	0.0459	1.53	0.086	0.0391	1.304
ADI(mg·kg^-1 bw)			0.01			0.03			0.03

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