Isolation and identification of a Pseudomonas strain and its application potential in rape straw composting in Qinghai, China

doi:10.3969/j.issn.1004-1524.2022.02.16

Acta Agriculturae Zhejiangensis ›› 2022, Vol. 34 ›› Issue (2): 343-351.DOI: 10.3969/j.issn.1004-1524.2022.02.16

• Environmental Science • Previous Articles Next Articles

Isolation and identification of a Pseudomonas strain and its application potential in rape straw composting in Qinghai, China

ZHANG Xinpeng(), WANG Xin, SUN Jian, YI Guoyun, LI Songling^*()

Academy of Agricultural and Forestry Sciences, Qinghai University, Xining 810016, China

Received:2021-05-06 Online:2022-02-25 Published:2022-03-02
Contact: LI Songling

Abstract

Abstract:

In order to accelerate the cellulose degradation of rape straw and improve the composting quality in Qinghai, China, a cellulose degrading strain was isolated from the soil of Beishan, Xining City, Qinghai Province, and was classified as the genus of Pseudomonas sp. Its carboxymethyl cellulase (CMC) activity, exo-β-glucanases activity, and filter paper activity (FPA) were 25.35, 22.33, 20.58 U·mL ^-1, respectively. The strain was made into a liquid microbial inoculum and applied in a composting test at the rate of 10 mL·kg^-1 as the experiment group. Compared with the control group without application of microbial inoculum, the stack of the experiment group heated up quickly and the high temperature lasted longer. At the end of composting, the pH value and electrical conductivity of the experiment group were within a reasonable range, the seed germination index of the experiment group was 90%, which met the maturity standard, the moisture content of the stack dropped to 30% below, and the contents of total nitrogen and organic matter were 1.39% and 49.16%, respectively. Therefore, the isolated strain could be used in the rape straw composting, which could both accelerate the rate and improve the quality of composting in Qinghai.

Key words: cellulose-degrading strain, microbial inoculum, composting

CLC Number:

S154.39

ZHANG Xinpeng, WANG Xin, SUN Jian, YI Guoyun, LI Songling. Isolation and identification of a Pseudomonas strain and its application potential in rape straw composting in Qinghai, China[J]. Acta Agriculturae Zhejiangensis, 2022, 34(2): 343-351.

Figures/Tables 11

References 19

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原料 Raw material	pH	含水率 Moisture content/%	有机质 Organic matter/%	全氮 Total N/%	全磷 Total P/%	全钾 Total K/%	C/N
猪粪 Pig manure	7.57	65.59	72.41	1.850	2.100	1.170	22.7∶1
油菜秸秆Rape straw	—	8.86	67.69	0.755	0.095	1.074	52.0∶1

原料 Raw material	pH	含水率 Moisture content/%	有机质 Organic matter/%	全氮 Total N/%	全磷 Total P/%	全钾 Total K/%	C/N
猪粪 Pig manure	7.57	65.59	72.41	1.850	2.100	1.170	22.7∶1
油菜秸秆Rape straw	—	8.86	67.69	0.755	0.095	1.074	52.0∶1

菌株Strain	D/cm	d/cm	D/d
HK-15-4	0.69±0.04	0.42±0.03	1.64±0.16
HK-15-5	0.59±0.04	0.38±0.03	1.57±0.07
HK-15-6	2.01±0.04	0.42±0.02	4.72±0.20
HK-15-7	1.27±0.04	0.60±0.02	2.11±0.03
HK-15-8	0.56±0.03	0.32±0.01	1.77±0.04

菌株Strain	D/cm	d/cm	D/d
HK-15-4	0.69±0.04	0.42±0.03	1.64±0.16
HK-15-5	0.59±0.04	0.38±0.03	1.57±0.07
HK-15-6	2.01±0.04	0.42±0.02	4.72±0.20
HK-15-7	1.27±0.04	0.60±0.02	2.11±0.03
HK-15-8	0.56±0.03	0.32±0.01	1.77±0.04

菌株 Strain	CMC酶活 CMC activity	滤纸酶活 FPA activity	外切-β-葡聚糖酶活 Exo-β-glucanases activity
HK-15-6	25.35±0.98	20.58±1.29	22.33±1.14
HK-15-7	16.22±1.12	15.81±1.34	17.36±1.17
HK-15-8	15.83±1.32	14.96±1.55	16.46±2.13

Isolation and identification of a Pseudomonas strain and its application potential in rape straw composting in Qinghai, China

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处理 Treatment	全氮Total nitrogen		有机质Organic matter
处理 Treatment	堆肥前 Before composting	堆肥后 After composting	堆肥前 Before composting	堆肥后 After composting
试验组Experiment group	1.36±0.09 aA	1.39±0.01 aA	68.05±1.61 aA	49.16±0.77 bB
对照组Control group	1.32±0.02 aA	1.29±0.04 aB	68.03±1.98 aA	55.53±0.65 bA

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