Effects of microplastics on growth and antioxidant system of ectomycorrhizal fungi

doi:10.3969/j.issn.1004-1524.2022.05.20

Acta Agriculturae Zhejiangensis ›› 2022, Vol. 34 ›› Issue (5): 1049-1060.DOI: 10.3969/j.issn.1004-1524.2022.05.20

• Environmental Science • Previous Articles Next Articles

Effects of microplastics on growth and antioxidant system of ectomycorrhizal fungi

LI Yuting¹(), LI Sha¹, CAO Jie¹, LI Jiaoyang¹, ZHANG Liang¹^,^*(), XU Xiaofeng¹^,²

1. School of Chemistry and Bioengineering, Nanjing Normal University Taizhou College, Taizhou 225300, Jiangsu, China
2. School of Life Sciences, Nanjing Normal University, Nanjing 210023, China

Received:2021-06-04 Online:2022-05-25 Published:2022-06-06
Contact: ZHANG Liang

Abstract

Abstract:

Microplastics pollution in soil and its toxicological effects have gradually attracted wide attention, but the toxicity of microplastics to ectomycorrhizal fungi (ECMF) is not clear yet. Therefore, Pisolithus tinctorius(Pt) and Lactarius delicius(Ld) were used as test strains in the present study, and monodisperse polystyrene microspheres (PS-MPs) with particle sizes of 80 nm and 4 μm were used as experimental materials. The effects of PS-MPs with different particle sizes and different concentrations (10-300 mg·L^-1) on the growth, malondialdehyde (MDA) content, soluble protein content, antioxidant enzymes activities and tissue conductivity of ECMF were explored by solid plate method and liquid culture method. The results showed that 200-300 mg·L^-1 PS-MPs significantly (P<0.05) inhibited the growth of Pt and Ld, and the PS-MPs with particle size of 4 μm showed stronger inhibition effect than PS-MPs with particle size of 80 nm. With the elevated concentration of PS-MPs, the superoxide dismutase activities of Pt and Ld exhibited an increasing first and then decreasing trend, and were all significantly (P<0.05) higher than that of control. When exposed to PS-MPs with different particle sizes, the catalase activity reached the lowest point under 300 mg·L^-1 PS-MPs treatment for Pt and Ld, and was significantly (P<0.05) lower than that of the control, while peroxidase activity was significantly (P<0.05) higher than that of control under all treatments. Besides, compared with the control, MDA content of Pt and Ld was significantly (P<0.05) elevated under other treatments (excluding Pt under 10 mg·L^-1 4 μm PS-MPs and Ld under 200 mg·L^-1 4 μm PS-MPs), soluble protein content was significantly (P<0.05) decreased (excluding Pt under 10, 20 mg·L^-1 80 nm PS-MPs and Ld under 10 mg·L^-1 80 nm PS-MPs), and the electrical conductivity of mycelium tissue was significantly (P<0.05) increased. Thus, it was inferred that the influence mechanism of PS-MPs on ECMF may involve oxidative stress, and different strains have different responses to PS-MPs with different particle sizes. These results would provide basis for the study regarding acute toxicity of microplastics on soil ECMF.

Key words: microplastics, ectomycorrhizal fungi, antioxidant enzymes

CLC Number:

X172

LI Yuting, LI Sha, CAO Jie, LI Jiaoyang, ZHANG Liang, XU Xiaofeng. Effects of microplastics on growth and antioxidant system of ectomycorrhizal fungi[J]. Acta Agriculturae Zhejiangensis, 2022, 34(5): 1049-1060.

Figures/Tables 7

Fig.1 Morphology and particle size distribution of polystyrene microspheres (PS-MPs) A,Transmission electron microscopy image of 80 nm PS-MPs; B,Scanning electron microscopy image of 4 μm PS-MPs; C,Particle size distribution of 80 nm PS-MPs; D, Particle size distribution of 4 μm PS-MPs.

Fig.2 Effect of polystyrene microspheres (PS-MPs) on growth of ectomycorrhizal fungi Pisolithus tinctorius (A) and Lactarius delicious (B)

Fig.3 Effect of polystyrene microspheres (PS-MPs) on biomass of ectomycorrhizal fungi Pisolithus tinctorius (A) and Lactarius delicious (B) Bars marked without the same letters indicated significant difference at P<0.05. The same as below.

Table 1 Effects of microplastics on SOD, CAT and POD activities of extomycorrhizal fangi

菌株 Strain	PS-MPs质量浓度 PS-MPs concentration/(mg·L^-1)	4 μm PS-MPs			80nm PS-MPs
菌株 Strain	PS-MPs质量浓度 PS-MPs concentration/(mg·L^-1)	SOD/ (U·g^-1)	CAT/ (U·g^-1)	POD/ (U·g^-1)	SOD/ (U·g^-1)	CAT/ (U·g^-1)	POD/ (U·g^-1)
Pt	0	14.23 i	118.65 a	39.15 d	14.23 i	118.65 b	39.15 h
	10	35.32 h	96.69 b	39.67 d	40.82 h	50.80 i	116.20 e
	20	45.21 f	79.90 c	62.59 c	39.63 h	81.25 f	142.15 c
	50	76.34 b	41.48 g	90.67 a	58.54 f	94.35 e	278.18 a
	100	67.86 c	37.67 g	91.81 a	87.42 c	48.21 i	132.82 cd
	200	62.12 d	55.81 e	11.22 f	70.17 d	36.77 j	101.43 f
	300	45.21 f	9.19 j	10.60 f	44.25 gh	20.02 k	122.68 de
Ld	0	17.56 i	59.26 e	22.45 e	17.56 i	59.26 h	22.45 i
	10	68.52 c	49.62 f	19.01 e	117.26 b	98.34 d	186.99 b
	20	84.22 ab	68.03 d	41.93 d	120.43 ab	109.64 c	130.68 d
	50	89.32 a	54.68 e	39.24 d	126.67 a	129.65 a	136.24 c
	100	69.82 c	46.71 f	82.76 b	91.46 c	127.04 a	138.22 c
	200	53.47 e	15.38 h	44.29 d	62.18 e	75.38 g	126.90 d
	300	40.36 g	5.36 i	13.30 f	48.91 g	14.01 k	67.55 g

Fig.4 Effect of polystyrene microspheres (PS-MPs) on malondialdehyde (MDA) content of ectomycorrhizal fungi Pisolithus tinctorius (A) and Lactarius delicious (B)

Fig.5 Effect of polystyrene microspheres (PS-MPs) on soluble protein content of ectomycorrhizal fungi Pisolithus tinctorius (A) and Lactarius delicious (B)

Fig.6 Effect of polystyrene microspheres (PS-MPs) on electrical conductivity of mycelium tissue of ectomycorrhizal fungi Pisolithus tinctorius (A) and Lactarius delicious (B)

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Effects of microplastics on growth and antioxidant system of ectomycorrhizal fungi

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