Development and application of KASP marker of BoCAL gene related to curd development in cauliflower

doi:10.3969/j.issn.1004-1524.2022.06.09

Acta Agriculturae Zhejiangensis ›› 2022, Vol. 34 ›› Issue (6): 1183-1192.DOI: 10.3969/j.issn.1004-1524.2022.06.09

• Horticultural Science • Previous Articles Next Articles

Development and application of KASP marker of BoCAL gene related to curd development in cauliflower

SHENG Xiaoguang(), SHEN Yusen, YU Huifang, WANG Jiansheng, ZHAO Zhenqing, GU Honghui()

Institute of Vegetable, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2022-02-15 Online:2022-06-25 Published:2022-06-30
Contact: GU Honghui

Abstract

Abstract:

BoCAL is one of the key genes controlling the curd formation in cauliflower. In order to improve the efficiency of BoCAL genotype identification of cauliflower, a competitive allele specific PCR (KASP) marker (BoCAL-KASP1) was developed according to the functional single nucleotide polymorphism (SNP) mutation of the 16th base (Pe5 + 16) G-T in the fifth exon of BoCAL. Using this KASP marker, BoCAL alleles were genotyped in 86 core accessions from seven variants of Brassica. oleracea and related wild species. It turned out that a total of thirty-six accessions showed the genotype of T:T, including twenty-eight cauliflower, six broccoli and two imported landraces; forty-three accessions had the genotype of G:G, including two cauliflower, nine broccoli, six cabbage, five Chinese kale, three kohlrabi, seven kale, two Brussels sprouts, four imported landraces and five related wild species; Seven accessions showed heterozygous genotype T:G, including two broccoli, two kale and three imported landraces. The results of KASP identification of BoCAL genotype of these 86 accessions were completely consistent with those of cleaved amplified polymorphic sequences (BoCAL-CAPS1) identification. Thus, the KASP marker developed in this study could efficiently and accurately identify the genotype of BoCAL target locus, so as to provide reliable functional markers for cauliflower curd development research and molecular breeding.

Key words: cauliflower, curd development, BoCAL gene, KASP marker

CLC Number:

S635.3

SHENG Xiaoguang, SHEN Yusen, YU Huifang, WANG Jiansheng, ZHAO Zhenqing, GU Honghui. Development and application of KASP marker of BoCAL gene related to curd development in cauliflower[J]. Acta Agriculturae Zhejiangensis, 2022, 34(6): 1183-1192.

Figures/Tables 6

References 28

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编号 No.	种质名称 Name	类型 Type	SNP	编号 No.	种质名称 Name	类型 Type	SNP	编号 No.	种质名称 Name	类型 Type	SNP
1/1	松花55-4 SH55-4	花椰菜 Cauliflower	T:T	30/8	极晚180 JW180	花椰菜 Cauliflower	G:G	59/35	苤蓝1号 PL1	苤蓝 Kohlrabi	G:G
2/2	农乐55 NL55	花椰菜 Cauliflower	T:T	31/9	青花60-2 QH60-2	青花菜 Broccoli	G:G	60/49	苤蓝2号 PL2	苤蓝 Kohlrabi	G:G
3/3	松花58-1 SH58-1	花椰菜 Cauliflower	T:T	32/10	阳光60-1 YG60-1	青花菜 Broccoli	G:G	61/39	苤蓝3号 PL3	苤蓝 Kohlrabi	G:G
4/4	松花60-2 SH60-2	花椰菜 Cauliflower	T:T	33/12	报春60 BC60	青花菜 Broccoli	G:G	62/41	羽甘3号 YG3	羽衣甘蓝 Kale	G:G
5/5	优松60-8 YS60-8	花椰菜 Cauliflower	T:T	34/13	秀美65 XM65	青花菜 Broccoli	G:G	63/38	羽甘6号YG6	羽衣甘蓝 Kale	G:T
6/6	松花65-1 SH65-1	花椰菜 Cauliflower	T:T	35/72	浙青65-4 ZQ65-4	青花菜 Broccoli	T:T	64/48	粉欧1号 FOU1	羽衣甘蓝 Kale	G:G
7/11	松花65-4 SH65-4	花椰菜 Cauliflower	T:T	36/73	青美70 QM70	青花菜 Broccoli	T:T	65/42	白鸥1号 BOU1	羽衣甘蓝 Kale	G:G
8/23	庆农65-3 QN65-3	花椰菜 Cauliflower	T:T	37/14	绿贵75 LG75	青花菜 Broccoli	G:G	66/80	紫鸽1号 ZGE1	羽衣甘蓝 Kale	G:G
9/24	佳丽佳65 JLJ65	花椰菜 Cauliflower	T:T	38/74	浙青75-9 ZQ75-9	青花菜 Broccoli	T:T	67/50	白珊瑚 White coral	羽衣甘蓝 Kale	G:T
10/28	松花68 SH68	花椰菜 Cauliflower	T:T	39/15	浙青80 ZQ80	青花菜 Broccoli	G:G	68/47	红珊瑚 Red coral	羽衣甘蓝 Kale	G:G
11/29	松花70-2 SH70-2	花椰菜 Cauliflower	T:T	40/16	喜鹊80 XQ80	青花菜 Broccoli	G:G	69/44	Kale-2	羽衣甘蓝 Kale	G:G
12/30	松花70-3 SH70-3	花椰菜 Cauliflower	T:T	41/17	报春85 BC85	青花菜 Broccoli	G:G	70/46	Kale-8	羽衣甘蓝 Kale	G:G
13/37	农胜70-1 NS70-1	花椰菜 Cauliflower	T:T	42/75	野崎90-6 YQ90-6	青花菜 Broccoli	T:T	71/45	王子 Prince	抱子甘蓝 Brussels sprout	G:G
14/55	优松70-1 YS70-1	花椰菜 Cauliflower	T:T	43/22	浙青95-4 ZQ95-4	青花菜 Broccoli	G:T	72/53	绿宝石 Emerald	抱子甘蓝 Brussels sprout	G:G
15/56	松花75-1 SH75-1	花椰菜 Cauliflower	T:T	44/18	野崎100 YQ100	青花菜 Broccoli	G:G	73/51	SG1913	地方种 Landrace	G:T
16/57	宝岛75 BD75	花椰菜 Cauliflower	T:T	45/40	浙青100-6 ZQ100-6	青花菜 Broccoli	G:T	74/64	SG1915	地方种 Landrace	G:G
17/58	松花78 SH78	花椰菜 Cauliflower	T:T	46/76	台绿3号 TL3	青花菜 Broccoli	T:T	75/54	SG1917	地方种 Landrace	G:T
18/59	橙松80-3 CS80-3	花椰菜 Cauliflower	T:T	47/82	台绿5号 TL5	青花菜 Broccoli	T:T	76/77	SG1401	地方种 Landrace	G:T
19/60	越优80-9 YY80-9	花椰菜 Cauliflower	T:T	48/19	中甘15号 ZG15	甘蓝 Cabbage	G:G	77/52	SG1501	地方种 Landrace	G:G
20/61	浙松80-5 ZS80-5	花椰菜 Cauliflower	T:T	49/20	中甘21号 ZG21	甘蓝 Cabbage	G:G	78/68	SG1521	地方种 Landrace	G:G
21/62	越优85-2 YY85-2	花椰菜 Cauliflower	T:T	50/21	浙甘85 ZG85	甘蓝 Cabbage	G:G	79/85	SG1528	地方种 Landrace	T:T
22/63	浙松88 ZS88	花椰菜 Cauliflower	T:T	51/25	苏甘8号 SG8	甘蓝 Cabbage	G:G	80/78	SG1539	地方种 Landrace	G:G
23/65	庆农90-4 QN90-4	花椰菜 Cauliflower	T:T	52/26	紫甘1号 ZG1	甘蓝 Cabbage	G:G	81/86	CR06	地方种 Landrace	T:T
24/66	松花90-7 SH90-7	花椰菜 Cauliflower	T:T	53/27	紫甘2号 ZG2	甘蓝 Cabbage	G:G	82/43	SP01	野生种 Wild species	G:G
25/67	浙松100 ZS100	花椰菜 Cauliflower	T:T	54/31	顺宝芥蓝 SB Kale	芥蓝 Chinese kale	G:G	83/81	SP9	野生种 Wild species	G:G
26/69	浙塔120-1 ZT120-2	花椰菜 Cauliflower	T:T	55/36	绿芥蓝 Green Kale	芥蓝 Chinese kale	G:G	84/83	SP12	野生种 Wild species	G:G
27/70	副将140-3 FJ140-3	花椰菜 Cauliflower	T:T	56/34	秋绿芥蓝 QL Kale	芥蓝 Chinese kale	G:G	85/84	SP16	野生种 Wild species	G:G
28/71	大圣140-3 DS140-3	花椰菜 Cauliflower	T:T	57/32	J1401	芥蓝 Chinese kale	G:G	86/79	SP20	野生种 Wild species	G:G
29/7	CR01	花椰菜 Cauliflower	G:G	58/33	J1402	芥蓝 Chinese kale	G:G

编号 No.	种质名称 Name	类型 Type	SNP	编号 No.	种质名称 Name	类型 Type	SNP	编号 No.	种质名称 Name	类型 Type	SNP
1/1	松花55-4 SH55-4	花椰菜 Cauliflower	T:T	30/8	极晚180 JW180	花椰菜 Cauliflower	G:G	59/35	苤蓝1号 PL1	苤蓝 Kohlrabi	G:G
2/2	农乐55 NL55	花椰菜 Cauliflower	T:T	31/9	青花60-2 QH60-2	青花菜 Broccoli	G:G	60/49	苤蓝2号 PL2	苤蓝 Kohlrabi	G:G
3/3	松花58-1 SH58-1	花椰菜 Cauliflower	T:T	32/10	阳光60-1 YG60-1	青花菜 Broccoli	G:G	61/39	苤蓝3号 PL3	苤蓝 Kohlrabi	G:G
4/4	松花60-2 SH60-2	花椰菜 Cauliflower	T:T	33/12	报春60 BC60	青花菜 Broccoli	G:G	62/41	羽甘3号 YG3	羽衣甘蓝 Kale	G:G
5/5	优松60-8 YS60-8	花椰菜 Cauliflower	T:T	34/13	秀美65 XM65	青花菜 Broccoli	G:G	63/38	羽甘6号YG6	羽衣甘蓝 Kale	G:T
6/6	松花65-1 SH65-1	花椰菜 Cauliflower	T:T	35/72	浙青65-4 ZQ65-4	青花菜 Broccoli	T:T	64/48	粉欧1号 FOU1	羽衣甘蓝 Kale	G:G
7/11	松花65-4 SH65-4	花椰菜 Cauliflower	T:T	36/73	青美70 QM70	青花菜 Broccoli	T:T	65/42	白鸥1号 BOU1	羽衣甘蓝 Kale	G:G
8/23	庆农65-3 QN65-3	花椰菜 Cauliflower	T:T	37/14	绿贵75 LG75	青花菜 Broccoli	G:G	66/80	紫鸽1号 ZGE1	羽衣甘蓝 Kale	G:G
9/24	佳丽佳65 JLJ65	花椰菜 Cauliflower	T:T	38/74	浙青75-9 ZQ75-9	青花菜 Broccoli	T:T	67/50	白珊瑚 White coral	羽衣甘蓝 Kale	G:T
10/28	松花68 SH68	花椰菜 Cauliflower	T:T	39/15	浙青80 ZQ80	青花菜 Broccoli	G:G	68/47	红珊瑚 Red coral	羽衣甘蓝 Kale	G:G
11/29	松花70-2 SH70-2	花椰菜 Cauliflower	T:T	40/16	喜鹊80 XQ80	青花菜 Broccoli	G:G	69/44	Kale-2	羽衣甘蓝 Kale	G:G
12/30	松花70-3 SH70-3	花椰菜 Cauliflower	T:T	41/17	报春85 BC85	青花菜 Broccoli	G:G	70/46	Kale-8	羽衣甘蓝 Kale	G:G
13/37	农胜70-1 NS70-1	花椰菜 Cauliflower	T:T	42/75	野崎90-6 YQ90-6	青花菜 Broccoli	T:T	71/45	王子 Prince	抱子甘蓝 Brussels sprout	G:G
14/55	优松70-1 YS70-1	花椰菜 Cauliflower	T:T	43/22	浙青95-4 ZQ95-4	青花菜 Broccoli	G:T	72/53	绿宝石 Emerald	抱子甘蓝 Brussels sprout	G:G
15/56	松花75-1 SH75-1	花椰菜 Cauliflower	T:T	44/18	野崎100 YQ100	青花菜 Broccoli	G:G	73/51	SG1913	地方种 Landrace	G:T
16/57	宝岛75 BD75	花椰菜 Cauliflower	T:T	45/40	浙青100-6 ZQ100-6	青花菜 Broccoli	G:T	74/64	SG1915	地方种 Landrace	G:G
17/58	松花78 SH78	花椰菜 Cauliflower	T:T	46/76	台绿3号 TL3	青花菜 Broccoli	T:T	75/54	SG1917	地方种 Landrace	G:T
18/59	橙松80-3 CS80-3	花椰菜 Cauliflower	T:T	47/82	台绿5号 TL5	青花菜 Broccoli	T:T	76/77	SG1401	地方种 Landrace	G:T
19/60	越优80-9 YY80-9	花椰菜 Cauliflower	T:T	48/19	中甘15号 ZG15	甘蓝 Cabbage	G:G	77/52	SG1501	地方种 Landrace	G:G
20/61	浙松80-5 ZS80-5	花椰菜 Cauliflower	T:T	49/20	中甘21号 ZG21	甘蓝 Cabbage	G:G	78/68	SG1521	地方种 Landrace	G:G
21/62	越优85-2 YY85-2	花椰菜 Cauliflower	T:T	50/21	浙甘85 ZG85	甘蓝 Cabbage	G:G	79/85	SG1528	地方种 Landrace	T:T
22/63	浙松88 ZS88	花椰菜 Cauliflower	T:T	51/25	苏甘8号 SG8	甘蓝 Cabbage	G:G	80/78	SG1539	地方种 Landrace	G:G
23/65	庆农90-4 QN90-4	花椰菜 Cauliflower	T:T	52/26	紫甘1号 ZG1	甘蓝 Cabbage	G:G	81/86	CR06	地方种 Landrace	T:T
24/66	松花90-7 SH90-7	花椰菜 Cauliflower	T:T	53/27	紫甘2号 ZG2	甘蓝 Cabbage	G:G	82/43	SP01	野生种 Wild species	G:G
25/67	浙松100 ZS100	花椰菜 Cauliflower	T:T	54/31	顺宝芥蓝 SB Kale	芥蓝 Chinese kale	G:G	83/81	SP9	野生种 Wild species	G:G
26/69	浙塔120-1 ZT120-2	花椰菜 Cauliflower	T:T	55/36	绿芥蓝 Green Kale	芥蓝 Chinese kale	G:G	84/83	SP12	野生种 Wild species	G:G
27/70	副将140-3 FJ140-3	花椰菜 Cauliflower	T:T	56/34	秋绿芥蓝 QL Kale	芥蓝 Chinese kale	G:G	85/84	SP16	野生种 Wild species	G:G
28/71	大圣140-3 DS140-3	花椰菜 Cauliflower	T:T	57/32	J1401	芥蓝 Chinese kale	G:G	86/79	SP20	野生种 Wild species	G:G
29/7	CR01	花椰菜 Cauliflower	G:G	58/33	J1402	芥蓝 Chinese kale	G:G

Development and application of KASP marker of BoCAL gene related to curd development in cauliflower

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