Effects of intestinal conditions and particle size on in vitro digestion of processed Carya cathayensis Sarg.

doi:10.3969/j.issn.1004-1524.2022.08.17

Acta Agriculturae Zhejiangensis ›› 2022, Vol. 34 ›› Issue (8): 1734-1742.DOI: 10.3969/j.issn.1004-1524.2022.08.17

• Food Science • Previous Articles Next Articles

Effects of intestinal conditions and particle size on in vitro digestion of processed Carya cathayensis Sarg.

SHAO Yuchen¹^,²(), MU Honglei², CHEN Hangjun², YIN Junyi¹, FANG Xiangjun², WU Weijie², LIU Ruiling², HAN Yanchao², GAO Haiyan¹^,²^,^*()

1. School of Food Science, Nanchang University, Nanchang 330036, China
2. Key Laboratory of Postharvest Handling of Fruits, Ministry of Agriculture and Rural Affairs, Key Laboratory of Postharvest Preservation and Processing of Fruits and Vegetables, China National Light Industry, Key Laboratory of Fruits and Vegetables Postharvest and Processing Technology Research of Zhejiang Province, Institute of Food Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

Received:2021-03-16 Online:2022-08-25 Published:2022-08-26
Contact: GAO Haiyan

Abstract

Abstract:

This article explored the effects of different intestinal conditions and particle size on in vitro digestion of processed Carya cathayensis Sarg.(CCS), matrix degradation rate (MDI), digestibility of fat and protein, total phenols and antioxidant activity of processed CCS were analyzed under different bile salt concentration, intestinal pH values and pancreatic lipase concentrations according to the intestinal conditions of healthy adults and people with exocrine pancreatic insufficiency (EPI). The results showed particle size had the most significant effect on processed CCS in vitro digestion, and the MDI, antioxidant activity and digestibility of fat and protein of small particle processed CCS were higher than those of large particle processed CCS. The pH and bile salt were associated with fat digestion and antioxidant activity. When pH value was 7 and bile salt concentration was 10 mmol·L^-1, the fat digestion effect was better. The digestibility of fat and protein would be high when the concentration of pancreatic lipase was high. Therefore, people with EPI can take in the appropriate amount of prebiotics and trypsin preparation to improve the nutrient digestion and utilization rate of CCS products.

Key words: Carya cathayensis Sarg., intestinal condition, particle size, in vitro digestion, antioxidant activity

CLC Number:

TS255.6

SHAO Yuchen, MU Honglei, CHEN Hangjun, YIN Junyi, FANG Xiangjun, WU Weijie, LIU Ruiling, HAN Yanchao, GAO Haiyan. Effects of intestinal conditions and particle size on in vitro digestion of processed Carya cathayensis Sarg.[J]. Acta Agriculturae Zhejiangensis, 2022, 34(8): 1734-1742.

Figures/Tables 7

Table 1 Influence of various factors and interactions on MDI,fat digestibility, protein digestibility, total phenols digestibility, DPPH radical and FARP antioxidant

影响因素与相互作用 Influence factors and interaction	粒径 Particle size	胆盐浓度 Bile salt concentration	pH	粒径-胆盐浓度 Particle size-Bile salt concentration	粒径-pH Particle size-pH	胆盐浓度-pH Bile salt concentration-pH	粒径-胆盐浓度-pH Particle size-Bile salt concentration-pH
MDI/%	471.44^***	6.44^*	5.97^*	1.07	0.01	6.44^*	0.32
脂肪消化率	4 602.43^***	26.83^***	454.04^***	4.04	9.05^**	69.56^***	11.55^**
Fat digestibility/%
蛋白质消化率	29.79^***	17.22^***	2.66	7.12^*	4.83^*	0.27	1.99
Protein digestibility/%
总酚	10.53^**	2.04	5.37^*	1.36	0.60	3.20	1.17
Total phenols/(mg·g^-1)
DPPH清除率	1 723.63^***	34.80^***	188.28^***	23.65^***	10.98^**	0.45	4.45
Scavenging of DPPH radical/%
FRAP/ (μmol·g^-1)	304.21^***	33.03^***	76.17^***	88.17^***	11.95^**	3.33	12.99^**

Fig.1 Effects of particle size and intestinal conditions on in vitro MDI of Carya cathayensis Sarg. The bars without the same lowercase letters show the significant difference (P<0.05). The same as below.

Fig.2 Effects of particle size and intestinal conditions on in vitro fat digestibility of Carya cathayensis Sarg.

Fig.3 Effects of particle size and intestinal conditions on in vitro protein digestibility of Carya cathayensis Sarg.

Fig.4 Effects of particle size and intestinal conditions on in vitro total phenols of Carya cathayensis Sarg.

Fig.5 Effects of particle size and intestinal conditions on in vitro scavenging of DPPH radical of Carya cathayensis Sarg.

Fig.6 Effects of particle size and intestinal conditions on in vitro FRAP antioxidant ability of Carya cathayensis Sarg.

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Effects of intestinal conditions and particle size on in vitro digestion of processed Carya cathayensis Sarg.

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