Effect of sodium nitrophenolate on flavonoid content and antioxidant activity in Dendrobium officinale

doi:10.3969/j.issn.1004-1524.20240325

Acta Agriculturae Zhejiangensis ›› 2025, Vol. 37 ›› Issue (4): 934-942.DOI: 10.3969/j.issn.1004-1524.20240325

• Quality and Safety of Agricultural Products • Previous Articles Next Articles

Effect of sodium nitrophenolate on flavonoid content and antioxidant activity in Dendrobium officinale

SUN Fengting¹^,²(), WANG Xu³, HAN Xinyu⁴, XU Zhenlan¹, WU Shenggan¹, HUANG Hao¹, TANG Tao¹, SHENG Qing², WANG Qiang¹, SHEN Guoqiang⁵^,^*(), ZHAO Xueping¹^,^*()

1. Key Laboratory for Pesticide Residue Detection, Ministry of Agriculture and Rural Affairs, Institute of Agro-Product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
2. College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China
3. Faculty of Materials Science and Chemistry, China University of Geosciences, Wuhan 430078, China
4. School of Food Science and Engineering, Hainan University, Haikou 570228, China
5. Institute of Resources and Environment, Shaoxing Academy of Agricultural Sciences, Shaoxing 312003, Zhejiang, China

Received:2024-04-11 Online:2025-04-25 Published:2025-05-09

Abstract

Abstract:

Flavonoids are one of the most significant bioactive constituents in Dendrobium officinale. To investigate the effects of sodium nitrophenolate on flavonoid content in D. officinale, one-year-old seedlings were treated with different concentrations of sodium nitrophenolate through foliar spraying, using distilled water as the control. Sampling was conducted at 0, 7, 15, 22, 30, 45, 60, 90, 120, 150, and 180 days (harvest period) after the final application. Total flavonoid content and antioxidant activity were measured, while 17 flavonoid compounds were quantitatively analyzed using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) coupled with the QuEChERS method. Results demonstrated that sodium nitrophenolate application enhanced both total flavonoid content and antioxidant activity in D. officinale stems. Furthermore, sodium nitrophenolate exhibited differential effects on the accumulation of individual flavonoid compounds. Specifically, it promoted the accumulation of 13 flavonoids including naringenin and eriodictyol. Correlation analysis revealed a positive relationship between antioxidant activity and total flavonoid content. These findings indicate that sodium nitrophenolate application can effectively improve the total flavonoid content in D. officinale, thereby enhancing its antioxidant activity.

Key words: Dendrobium officinale, sodium nitrophenolate, flavonoid, ultra-performance liquid chromatography-tandem mass spectrometry, antioxidant activity

CLC Number:

S567.239

SUN Fengting, WANG Xu, HAN Xinyu, XU Zhenlan, WU Shenggan, HUANG Hao, TANG Tao, SHENG Qing, WANG Qiang, SHEN Guoqiang, ZHAO Xueping. Effect of sodium nitrophenolate on flavonoid content and antioxidant activity in Dendrobium officinale[J]. Acta Agriculturae Zhejiangensis, 2025, 37(4): 934-942.

Figures/Tables 3

Fig.1 Effect of sodium nitrophenolate on the content of total flavonoids in Dendrobium officinale ** indicates P<0.01, and *** indicates P<0.001.

Fig.2 Effect of sodium syndiclate on the content of 17 flavonoid compounds in Dendrobium officinale a, Naringenin; b, Rutin; c, Schaftoside; d, Quercetin; e, Eriodictyol; f, Apigenin; g, Isorhamnetin; h, Chrysoeriol; i, Hesperetin; j, Isoquercitrin; k, Quercetin; l, Chrysin; m, Luteoloside; n, Kaempferol; o, Apiin; p, Naringin; q, Taxifolin. * indicates P<0.05, ** indicates P<0,01, and *** indicates P<0.001.

Fig.3 Effect of sodium syndiclate on DPPH radical scavenging capacity, ABTS+ radical scavenging capacity, and FRAP total antioxidant capacity in Dendrobium officinale

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Effect of sodium nitrophenolate on flavonoid content and antioxidant activity in Dendrobium officinale

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